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Development of an effective treatment for the reduction of Campylobacter jejuni on chicken carcasses by resonant ultrasonication and microbubbles

Research Project

Project/Area Number 21580376
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionUniversity of Miyazaki

Principal Investigator

MISAWA Naoaki  宮崎大学, 農学部, 教授 (20229678)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords食品 / 細菌 / 獣医学 / マイクロバブル / 超音波
Research Abstract

Healthy chickens are considered as reservoirs for campylobacters and the organisms colonize in the intestinal tract. Contamination of chicken carcasses with C. jejuni and C. coli occurs over the entire skin surface during processing. In this study, an effective treatment for decontaminating campylobacters using naturally contaminated chicken carcasses was developed. The present study showed that combinations of decontamination techniques of chicken carcasses immerse in cetylpiridinium chloride, vacuum, and ultrasonic generator for water resonance, were highly effective to reduce campylobacters on the skins of carcasses with naturally contaminated chickens.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (55 results)

All 2012 2011 2010 2009 Other

All Journal Article (17 results) (of which Peer Reviewed: 16 results) Presentation (26 results) Book (7 results) Remarks (1 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 2 results)

  • [Journal Article] Correlations between 23S rRNA genes and erythromycin resistance in Campylobacter jejuni.2012

    • Author(s)
      Tazumi, A., Xu, J., Han, L., Misawa, N., Moore, J., Millar, C., and Matsuda, M
    • Journal Title

      Ann. Microbiol.

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Correlations between 23S rRNA genes and erythromycin resistance in Campylobacter jejuni2012

    • Author(s)
      Tazumi, A., Xu, J., Han, L., Misawa, N., Moore, J., Millar, C., Matsuda, M.
    • Journal Title

      Annals of Microbiology.Ann.Microbiol.

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Uneven distribution of the luxS gene within the genus Campylobacter2011

    • Author(s)
      Tazumi, A., Negoro, M., Tomiyama, Y., Misawa, N., Itoh, K., Moore, J. E., Millar, B. C., Matsuda, M
    • Journal Title

      Br. J. Biomed. Sci

      Volume: 68 Pages: 19-22

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Qfever in Japan : An update review2011

    • Author(s)
      Porter, S. R., Czaplicki, G., Mainil, J., Horii, Y., Misawa, N, and Saegerman, C
    • Journal Title

      Vet. Microbiol

      Volume: 149 Pages: 198-306

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Uneven distribution of the luxS gene within the genus Campylobacter2011

    • Author(s)
      Tazumi, A., Negoro, M., Tomiyama, Y., Misawa, N., Itoh, K., Moore, J.E., Millar, B.C., Matsuda, M.
    • Journal Title

      Br.J.Biomed.Sci.

      Volume: 68 Pages: 19-22

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 超音波を使用したカンピロバクター撃退技術2010

    • Author(s)
      三澤尚明、副島潤一郎
    • Journal Title

      超音波TECHNO

      Volume: 22 Pages: 6-9

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Journal Article] The mode of biofilm formation on smooth surfaces by Campylobacter jejuni2010

    • Author(s)
      Moe, K. K., Mimura, J., Ohnishi, T., Wake, T., Yamazaki, W., Masaaki Nakai, M., and Misawa, N
    • Journal Title

      J. Vet. Med. Sci

      Volume: 72 Pages: 411-416

    • NAID

      130000149937

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Uneven distribution of luxS gene within the genus Campylobacter.2010

    • Author(s)
      Tazymi, A., Negoro, M., Tomiyama, N., Misawa, N., Itoh, K., Moore, J.E., Millar, B.C., Matsuda, M.
    • Journal Title

      Br.J.Biomed.Sci.

      Volume: 68 Pages: 19-22

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of loop-mediated isothermal amplification and PCR assays for rapid and simple detection of Campylobacter fetus subsp.venerealis.2010

    • Author(s)
      Yamazaki, W., Taguchi, M., Misawa, N.
    • Journal Title

      Microbiol.Immunol.

      Volume: 54 Pages: 398-404

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of antibodies against Fusobacteriumnecrophorum and Porphyromonaslevii-like species in dairy cattle with papillomatous digital dermatitis2010

    • Author(s)
      Moe, K.K., T.Yano, K.Misumi, C.Kubota, K.Nibe, W.Yamazaki, M.Muguruma, Misawa, N.
    • Journal Title

      Microbiol.Immunol.

      Volume: 54 Pages: 338-346

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of candidate pathogens of papillomatous digital dermatitis in dairy cattle from quantitative 16S rRNA clonal analysis2010

    • Author(s)
      Yano, T., Moe, K.K., Yamazaki, K., Ooka, T., Hayashi, T., Misawa, N.
    • Journal Title

      Vet.Microbiol.

      Volume: 143 Pages: 352-362

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The mode of biofilm formation on smooth surfaces by Campylobacter jejuni2010

    • Author(s)
      Moe, K.K., Mimura, J., Ohnishi, T., Wake, T., Yamazaki, W., Nakai, M., Misawa, N.
    • Journal Title

      J.Vet.Med.Sci.

      Volume: 72 Pages: 411-416

    • NAID

      130000149937

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Q fever in Japan : An update review2010

    • Author(s)
      Porter, S.R., Czaplicki, G., Mainil, J., Horii, Y., Misawa, N, Saegerman, C
    • Journal Title

      Vet.Microbiol.

      Volume: DOI:10.1016/j.vetmic.2010.11.017

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of the tdh and trh genes in Vibrio parahaemolyticus and related Vibrio species2009

    • Author(s)
      Yamazaki, W., Kumeda, Y., Misawa, N., Nakaguchi, Y., and Nishibuchi, M
    • Journal Title

      Appl. Environ. Microbiol

      Volume: 76 Pages: 820-828

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 Campylobacter isolates from seven species including atypical campylobacters2009

    • Author(s)
      Tazumi, A., Kakinuma, Y., Misawa, N., Moore, J., Millar, J. B. C., and Matsuda, M
    • Journal Title

      BMC Microbiology

      Volume: 9 Issue: 1 Pages: 256-256

    • DOI

      10.1186/1471-2180-9-256

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Absence of intervening sequences(IVSs) in helix 11 region within 16S rRNA genes among more than 240 isolates of the seven Campylobacter species2009

    • Author(s)
      Sekizuka, A., Tazumi, A., Nakanishi, S., Meguro, S., Kakinuma, Y., Misawa, N., Moore, J. E., Millar, B. C., Matsuda, M
    • Journal Title

      Br. J. Biomed. Sci

      Volume: 66 Pages: 103-106

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of Campylobacter jejuni and Campylobacter coli detection in naturally contaminated chicken meat samples between loop-mediated isothermal amplification assay and conventional culture methods2009

    • Author(s)
      Yamazaki, W., Taguchi, M., Kawai, T., Kawatsu, K., Sakata, J., Inoue, K., Misawa, N
    • Journal Title

      Appl. Environ. Microbiol

      Volume: 75 Pages: 1597-1603

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] 食鳥と体皮膚の各処理工程におけるカンピロバクターの定量検査と冷却前後の羽毛包の観察2011

    • Author(s)
      浦田彩香、新木泰輔、佐々木賢美、谷口喬子、山崎渉、三澤尚明
    • Organizer
      日本獣医公衆衛生学会(九州)
    • Place of Presentation
      長崎市
    • Year and Date
      2011-10-30
    • Related Report
      2011 Final Research Report
  • [Presentation] 食鳥と体皮膚の各処理工程におけるカンピロバクターの定量検査と冷却前後の羽毛包の観察2011

    • Author(s)
      浦田彩香、新木泰輔、佐々木賢美、谷口喬子、山崎渉、三澤尚明
    • Organizer
      日本獣医公衆衛生学会(九州)
    • Place of Presentation
      長崎市(長崎県)
    • Year and Date
      2011-10-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] げっ歯類の糞便から分離されたHelicobacter cinaediとヒト由来株との比較2011

    • Author(s)
      比嘉真理子、谷口喬子、山崎渉、三澤尚明
    • Organizer
      日本獣医公衆衛生学会(九州)
    • Place of Presentation
      長崎市(長崎県)
    • Year and Date
      2011-10-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] 食鳥処理工程におけるブロイラーと体皮膚へのCampylobacter jejuniの付着能について2011

    • Author(s)
      大城昇太郎、新木泰輔、谷口喬子、山崎渉、三澤尚明
    • Organizer
      第152回日本獣医学科学術集会
    • Place of Presentation
      大阪府
    • Year and Date
      2011-09-20
    • Related Report
      2011 Final Research Report
  • [Presentation] 家兎における牛の趾乳頭腫症病変モデル作出の試み2011

    • Author(s)
      福田圭祐、後藤恭宏、林哲也、谷口喬子、山崎渉、吉谷一紀、三澤尚明
    • Organizer
      第152回日本獣医学科学術集会
    • Place of Presentation
      堺市(大阪府)
    • Year and Date
      2011-09-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] 食鳥処理工程におけるブロイラーと体皮膚へのCampylobacter jejuniの付着能について2011

    • Author(s)
      大城昇太郎、新木泰輔、谷口喬子、山崎渉、三澤尚明
    • Organizer
      第152回日本獣医学科学術集会
    • Place of Presentation
      堺市(大阪府)
    • Year and Date
      2011-09-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Identification and typing of Helicobacter cinaedi strains isolated from humans and animals by MALDI-TOF mass spectrometry2011

    • Author(s)
      Misawa, N., Taniguchi, T., Yamazaki, W., Saeki, Y., Umeki, K., Okayama, A., Nakayama, K., Hayashi, T.
    • Organizer
      International Union of Microbiological Society
    • Place of Presentation
      札幌市(北海道)(招待講演)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Helicobacter new genospecies fund in animal (dog) isolates identified as Helicobacter cinaedi2011

    • Author(s)
      Kawamura, Y., Fujitaka, Y., Nakayama, K., Misawa, N., Kamiya, Y., Tomida, J., Morita, Y., Hayashi, T., Akaike, T.
    • Organizer
      International Union of Microbiological Society
    • Place of Presentation
      札幌市(北海道)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Genome sequence analysis of Treponema phagedenis isolated from bovine papillomatous digital dermatitis (PDD)2011

    • Author(s)
      Gotho, Y., Yamazaki, K., Ogura, Y., Ooka, T., Yano, T., Oshima, K., Hattori, M., Misawa, N., Hayashi, T.
    • Organizer
      International Union of Microbiological Society
    • Place of Presentation
      札幌市(北海道)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Development of an effective decontamination of Campylobacter species on chicken carcasses2011

    • Author(s)
      Misawa, N., Shinki, T., Sasaki, S., Taniguchi, T., Yamazaki, W
    • Organizer
      16th International Campylobacter, Helicobacter, and related organisms, Vancouver
    • Place of Presentation
      Canada
    • Year and Date
      2011-08-30
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of an effective decontamination of Campylobacter species on chicken carcasses2011

    • Author(s)
      Misawa, N., Shinki, T., Sasaki, S., Taniguchi, T., Yamazaki, W.
    • Organizer
      16^<th> International Campylobacter, Helicobacter, and related organisms
    • Place of Presentation
      バンクーバー(カナダ)
    • Year and Date
      2011-08-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Identification and typing of Helicobacter cinaedi strains isolated from humans and animals by MALDI-TOF mass spectrometry2011

    • Author(s)
      Taniguchi, T., Higa, m., Saeki, Y., Umeki, K., Okayama, A., Nakayama, K., Yamazaki, W., Misawa, N.
    • Organizer
      16^<th> International Campylobacter, Helicobacter, and related organisms
    • Place of Presentation
      バンクーバー(カナダ)
    • Year and Date
      2011-08-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] 新しい概念に基づく食鳥と体のカンピロバクター制御法の開発2010

    • Author(s)
      三澤尚明
    • Organizer
      第3回日本カンピロバクター研究会
    • Place of Presentation
      宮崎市
    • Year and Date
      2010-12-03
    • Related Report
      2011 Final Research Report
  • [Presentation] 新しい概念に基づく食鳥と体のカンピロバクター制御法の開発2010

    • Author(s)
      三澤尚明
    • Organizer
      第3回日本カンピロバクター研究会(宮崎市)
    • Place of Presentation
      宮崎県宮崎市
    • Year and Date
      2010-12-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] オゾン含有マイクロバブル水によるマンゴーの鮮度保持並びに殺菌効果2010

    • Author(s)
      三澤尚明、佐々木賢美、吉山佳世、福井敬一
    • Organizer
      日本食品微生物学会
    • Place of Presentation
      大津市
    • Year and Date
      2010-11-11
    • Related Report
      2011 Final Research Report
  • [Presentation] 食鳥肉における新しいカンピロバクター制御技術の開発2010

    • Author(s)
      新木泰輔、佐々木賢美、山崎渉、三澤尚明
    • Organizer
      日本食品微生物学会
    • Place of Presentation
      大津市
    • Year and Date
      2010-11-11
    • Related Report
      2011 Final Research Report
  • [Presentation] 食鳥肉における新しいカンピロバクター制御技術の開発2010

    • Author(s)
      新木泰輔、佐々木賢美、山崎渉、三澤尚明
    • Organizer
      日本食品微生物学会
    • Place of Presentation
      滋賀県大津市
    • Year and Date
      2010-11-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] オゾン含有マイクロバブル水によるマンゴーの鮮度保持並びに殺菌効果2010

    • Author(s)
      三澤尚明、佐々木賢美、吉山佳世、福井敬一
    • Organizer
      日本食品微生物学会
    • Place of Presentation
      滋賀県大津市
    • Year and Date
      2010-11-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] LAMP法による腸炎ビブリオ病原因子tdh,trh1,trh2ならびに菌種特異的遺伝子の簡易迅速検出法の開発2010

    • Author(s)
      山崎渉、三澤尚明、中口義次、西渕光昭
    • Organizer
      平成22年度日本獣医公衆衛生学会(九州)
    • Place of Presentation
      佐賀県佐賀市
    • Year and Date
      2010-10-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 血清中に含まれるCampylobacter jejuniの自発凝集活性促進物質の検出2010

    • Author(s)
      吉本有貴、山崎渉、三澤尚明
    • Organizer
      日本獣医学会学術集会
    • Place of Presentation
      帯広市
    • Year and Date
      2010-09-16
    • Related Report
      2011 Final Research Report
  • [Presentation] 鶏と体のカンピロバクター制御技術の開発2010

    • Author(s)
      三澤尚明、佐々木賢美、山崎渉
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広市
    • Year and Date
      2010-09-16
    • Related Report
      2011 Final Research Report
  • [Presentation] 鶏と体のカンピロバクター制御技術の開発2010

    • Author(s)
      三澤尚明、佐々木賢美、山崎渉
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      北海道帯広市
    • Year and Date
      2010-09-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 血清中に含まれるCampylobacter jejuniの自発凝集活性促進物質の検出2010

    • Author(s)
      吉本有貴、山崎渉、三澤尚明
    • Organizer
      日本獣医学会学術集会
    • Place of Presentation
      北海道帯広市
    • Year and Date
      2010-09-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 牛趾乳頭腫症由来Treponemaphagedenisの上皮細胞侵入機構の解析2010

    • Author(s)
      三澤尚明、大町真理、竹貞友恵、岩田喬子、山崎渉、林哲也
    • Organizer
      日本細菌学会九州支部総会
    • Place of Presentation
      宮崎県宮崎市
    • Year and Date
      2010-09-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] 食肉の衛生管理について-食鳥肉におけるカンピロバクターの制御を中心に-2010

    • Author(s)
      三澤尚明
    • Organizer
      大分県公衆衛生獣医師協議会研修会
    • Place of Presentation
      大分市
    • Year and Date
      2010-06-01
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of an effective treatment for the reduction of Campylobacter jejuni on chicken carcasses2010

    • Author(s)
      Misawa, N., T.Shinki, T., Sasaki, S., Yamamoto, S., Taniguchi, T., Yamazaki, W.
    • Organizer
      45th Conference Cholera and other bacterial enteric infections US-Japan cooperative medical science program
    • Place of Presentation
      京都府京都市
    • Related Report
      2010 Annual Research Report
  • [Book] 動物の感染症第三版2011

    • Author(s)
      三澤尚明
    • Total Pages
      131
    • Publisher
      近代出版(東京)
    • Related Report
      2011 Final Research Report
  • [Book] 獣医微生物学第3版2011

    • Author(s)
      三澤尚明
    • Publisher
      文永堂出版(東京)
    • Related Report
      2011 Final Research Report
  • [Book] 新・食肉衛生検査マニュアル全国食肉衛生検査所協議会編2011

    • Author(s)
      三澤尚明
    • Publisher
      中央法規出版(東京)
    • Related Report
      2011 Final Research Report
  • [Book] 新・食肉衛生検査マニュアル2011

    • Author(s)
      三澤尚明(分担執筆)
    • Total Pages
      481
    • Publisher
      中央法規出版(東京)
    • Related Report
      2011 Annual Research Report
  • [Book] 獣医微生物学第3版2011

    • Author(s)
      三澤尚明(分担執筆)
    • Total Pages
      452
    • Publisher
      文永堂出版(東京)
    • Related Report
      2011 Annual Research Report
  • [Book] 獣医公衆衛生学実習2010

    • Author(s)
      三澤尚明
    • Publisher
      学窓社
    • Related Report
      2010 Annual Research Report
  • [Book] 動物の感染症第3版2009

    • Author(s)
      三澤尚明
    • Total Pages
      131
    • Publisher
      近代出版(東京)
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] 真空及び共振型超音波処理による食品材料における微生物の制御方法及び制御装置2011

    • Inventor(s)
      三澤尚明、副島潤一郎、小山克宏
    • Industrial Property Rights Holder
      宮崎大学、株・カイジョー
    • Industrial Property Number
      2012-507123
    • Filing Date
      2011-03-18
    • Related Report
      2011 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 口蹄疫ウイルスの包括的検出法2011

    • Inventor(s)
      山崎渉、三澤尚明
    • Industrial Property Rights Holder
      宮崎大学
    • Industrial Property Number
      2012-003740
    • Filing Date
      2011-01-12
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] 真空及び共振型超音波処理による食品材料における微生物の制御法及び制御装置2011

    • Inventor(s)
      三澤尚明, 副島潤一郎
    • Industrial Property Rights Holder
      宮崎大学、(株)カイジョー
    • Filing Date
      2011-03-18
    • Related Report
      2010 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 真空及び共振型超音波処理による食品材料における微生物の制御方法及び制御装置2010

    • Inventor(s)
      三澤尚明, 副島潤一郎, 小山克宏
    • Industrial Property Rights Holder
      宮崎大学(株)カイジョー
    • Industrial Property Number
      2010-065744
    • Filing Date
      2010-03-23
    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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