Investigation of pathophysiologic mechanisms of acute cardiogenic shock by necrotizing toxins from gas gangrene bacilli.

• Project/Area Number: 21580379
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Osaka Prefecture University
• Principal Investigator: MUKAMOTO Masafumi 大阪府立大学, 生命環境科学研究科, 准教授 (80231629)
• Co-Investigator(Kenkyū-buntansha): KOZAKI Shunji 大阪府立大学, 生命環境科学研究科, 教授 (10109895)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 悪性水腫 / クロストリジウム / 細胞壊死毒素 / 心臓 / 心不全 / セプチカム

Research Abstract

Contraction of cultured cardiomyocytes from rat fetus heart ventricles have been stopped by pore formation of alpha-toxin on cell membranes. It was suggested that decrease of ATP productions and desiccation of ATP in cardiomyocytes have been caused by mitochondrial myopathy from Ca^<2+> excess in cytoplasm. It has been clear that tryptophan of 338th and 342th, hydrophobic amino acids of 340th and negative charged amino acids of 339th play an important roles in cell binding and cytotoxic activities of alpha-toxin.

Research Products

• [Journal Article] Characterization of the D/C mosaic neurotoxin produced by Clostridium botulinum associated with bovine botulism in Japan (2010)
  • Author(s): Nakamura K, Kohda T, Umeda K, Yamamoto H, Mukamoto M, Kozaki S
  • Journal Title: Vet. Microbiol. Volume: 140 Pages: 147-154
  • Peer Reviewed
• [Presentation] Clostridium septicumα毒素による心原性ショックの病態生理機構の解明 (2010)
  • Author(s): 向本雅郁、松村朋恵、幸田知子、小崎俊司
  • Organizer: 第57回トキシンシンポジウム
  • Place of Presentation: 長浜市
  • Year and Date: 2010-07-15
• [Presentation] Clostridium septicumα毒素による心原性ショックのボウタイ生理機構の解明 (2010)
  • Author(s): 向本雅郁、小崎俊司
  • Organizer: 第57回トキシンシンポジウム
  • Place of Presentation: 滋賀県長浜ロイヤルホテル
  • Year and Date: 2010-07-15
• [Book] 微生物胞子 (2011)
  • Author(s): 向本雅郁
  • Total Pages: 370
  • Publisher: 株式会社サイエンスフォーラム
• [Book] 微生物胞子(渡部一仁、土戸哲明、坂上吉一編) (2011)
  • Author(s): 向本雅郁
  • Total Pages: 370
  • Publisher: サイエンスフォーラム