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A cell biological study of immunological diversity in bovine mammary tissue

Research Project

Project/Area Number 21580380
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionRakuno Gakuen University

Principal Investigator

HIGUCHI Hidetoshi  酪農学園大学, 獣医学部, 准教授 (00305905)

Co-Investigator(Kenkyū-buntansha) 岩野 英知  酪農学園大学, 獣医学群, 准教授 (60382488)
中島 恵一  (独)農業・食品産業技術総合研究機構, 北海道農業研究センター, 主任研究員 (70362150)
小酒井 貴晴  山形大学, 地域教育文化学部, 准教授 (70391480)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsウシ / 乳房炎 / 乳腺上皮細胞 / 感染防御 / 乳房炎病原細菌 / ラクトフェリン / 好中球 / マクロファージ / 乳腺細胞 / 情報伝達系 / 黄色ブドウ球菌 / 免疫 / 三次元培養 / サイトカイン
Research Abstract

We created a cell culture model as a novel model of bovine mammary infection. Using this model, it was clarified that mammary epithelial cells recognized to date as milk-producing cells possess immunological diversity and are closely related to immunocompetent cells. The topic of this application is developmental research in preventative veterinary medicine and the findings are of great social significance to steady development of primary industry in Japan.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (33 results)

All 2011 2010 2009 2000 Other

All Journal Article (16 results) (of which Peer Reviewed: 8 results) Presentation (17 results)

  • [Journal Article] Prevalence of Mycoplasma species in bulk tank milk in Japan2011

    • Author(s)
      Higuchi, H., Iwano, H., Kawai, K., Gondaira, T., Nagahata, H.
    • Journal Title

      Vet. Rec

      Volume: 169 Pages: 442-442

    • Related Report
      2011 Final Research Report
  • [Journal Article] A simplified PCR assay for fast and easy screening of Mycoplasma mastitis of dairy cattle2011

    • Author(s)
      Higuchi, H., Iwano, H., Kawai, K., Ohta, T., Obayashi, T., Hirose, K, Ito, N., Yokota, H., Tamura, Y., Nagahata, H.
    • Journal Title

      J. Vet. Sci

      Volume: 12 Pages: 191-193

    • Related Report
      2011 Final Research Report
  • [Journal Article] Analysis of Trace and Major Elements in Bronchoalveolar Lavage Fluid of Mycoplasma Bronchopneumonia in Calves2011

    • Author(s)
      Suzuki, K., Higuchi, H., Iwano, H., Lakritz, J., Sera, K., Koiwa, M., Taguchi, K.
    • Journal Title

      Biol. Trace. Elem. Res

      Volume: 145 Pages: 166-171

    • Related Report
      2011 Final Research Report
  • [Journal Article] A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle2011

    • Author(s)
      Higuchi, H., Iwano, H., Kawai, K., Ohta, T., Obayashi, et al
    • Journal Title

      Journal of Veterinary Science

      Volume: 12 Pages: 191-193

    • NAID

      120005761687

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of Mycoplasma species in bulk tank milk in Japan2011

    • Author(s)
      Higuchi, H., Iwano, H., Gondaira, S., Kawai, K., Nagahata, H
    • Journal Title

      Veterinary Record

      Volume: 169 Pages: 442-442

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Altered leukocyte responsiveness in dairy cows with naturally occurring chronic Staphylococcus aureus mastitis2011

    • Author(s)
      Nagahata, H., Kawai, H., Higuchi, H., Kawai, K., et al
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 73

    • NAID

      130000676901

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Mycotoxin on Chemiluminescent Response and Cytokine mRNA Expression on Bovine Neutrophils2010

    • Author(s)
      Wada, K., Nabae, A., Higuchi, H., Nagahata, M.
    • Journal Title

      J. Vet. Med. Sci

      Volume: 72 Pages: 1507-1511

    • NAID

      130000307669

    • Related Report
      2011 Final Research Report
  • [Journal Article] A retrospective survey of the prevalence of complex vertebral malformation carriers in 9 Holstein dairy herds in Hokkaido, Japan2010

    • Author(s)
      Nagahata, H., Nishiyama, T., Kanae, Y., Higuchi, H., Kawai, K., Endoh, D., Hayashi, M., Kurosawa, T.
    • Journal Title

      J. Vet. Med. Sci

      Volume: 71 Pages: 793-795

    • NAID

      130000134405

    • Related Report
      2011 Final Research Report
  • [Journal Article] Effects of ammonia and hydrogen sulfide on physical and biochemical properties of the claw horn of Holstein cows2009

    • Author(s)
      Higuchi, H., Kurumado, H., Mori, M., Degawa, A., Fujisawa, H., Kuwano, A. and Nagahata, H.
    • Journal Title

      Can. J. Vet. Res

      Volume: 73 Pages: 15-20

    • Related Report
      2011 Final Research Report
  • [Journal Article] Comparison of serum lipid compositions, lipid peroxide,α-tocopherol and lipoproteins in captive marine mammals(bottlenose dolphins, spotted seals and West Indian manatees) and terrestrial mammals2009

    • Author(s)
      Kasamatsu, M., Kawauchi, R., Tsunokawa, M., Ueda, K., Uchida, E., Oikawa, S., Higuchi, H., Kawajiri, T., Uchida, S. and Nagahata, H.
    • Journal Title

      Res. Vet. Sci

      Volume: 86 Pages: 216-222

    • Related Report
      2011 Final Research Report
  • [Journal Article] Characterization of bacterial population of raw milk from bovine mastitis by culture-independent PCR-DGGE method2009

    • Author(s)
      Kuang, Y., Tani, K., Synnott, A. J., Ohshima, K., Higuchi, H., Nagahata, H. and Tanji, Y.
    • Journal Title

      Biochemical Engineering Journal

      Volume: 45 Pages: 76-81

    • NAID

      120006582021

    • Related Report
      2011 Final Research Report
  • [Journal Article] Effects of ammonia and hydrogen sulfide on physical and biochemical properties of the claw horn of Holstein cows.2009

    • Author(s)
      Higuchi H, Kurumado H, Mori M, Degawa A, Fujisawa H, Kuwano A, Nagahata H.
    • Journal Title

      Can.J.Vet.Res. 73

      Pages: 15-20

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of bacterial population of raw milk from bovine mastitis by culture-dependent PCR DGGE method.2009

    • Author(s)
      Kaung Y, Tani K, Synnott A, Ohshina K, Higuchi H, Ngahata H, Tanji Y.
    • Journal Title

      Biochem Engineer.J. 45

      Pages: 76-81

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation from sewage influent and characterization of novel Staphylococcus aureus bacteriophages with wide host ranges and potent lytic capabilities.2009

    • Author(s)
      Synnott A, Kaung Y, Kurimoto M, Yamamichi K, Iwano H, Tanji Y.
    • Journal Title

      Appl.Environ. Microbiol. 75

      Pages: 4483-4490

    • NAID

      120006581987

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Mycotoxin on Chemiluminescent Response and Cytokine mRNA Expression on Bovine Neutrophils2000

    • Author(s)
      Wada, K., Nabae, A., Higuchi, H., Nagahata, M.
    • Journal Title

      Journal of Veterinary Medical Association

      Volume: 72 Pages: 1507-1511

    • NAID

      130000307669

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] simplified PCR assay for fast and easy screening of Mycoplasma mastitis of dairy cattle

    • Author(s)
      Higuchi, H., Iwano, H., Kawai, K., Ohta, T., Obayashi, T., Hirose, K, Ito, N., Yokota, H., Tamura, Y., Nagahata, H
    • Journal Title

      Journal of Veterinary Science

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] マイコプラズマ性乳房炎の摘発を目的としたバルクスクリーニングの実施とその評価2011

    • Author(s)
      樋口豪紀・岩野英知・河合一洋・馬場幸宏・古岡みゆき、斉藤哲・佐藤豊孝・大林哲・小岩政照・横田博・田村豊・永幡肇
    • Organizer
      北海道獣医師会
    • Related Report
      2011 Final Research Report
  • [Presentation] 内視鏡による子牛中耳炎の病態評価および病態と臨床症状の比較2011

    • Author(s)
      小岩政照、安藤貴朗、甲斐貴憲、樋口豪紀
    • Organizer
      北海道獣医師会
    • Related Report
      2011 Final Research Report
  • [Presentation] 子牛のマイコプラズマ性中耳炎に対する治療法の検討2011

    • Author(s)
      甲斐貴憲、安藤貴朗、樋口豪紀、小岩政照
    • Organizer
      北海道獣医師会
    • Related Report
      2011 Final Research Report
  • [Presentation] ウシラクトフェリンが骨の血管新生因子の発現誘導に及ぼす影響2011

    • Author(s)
      中島恵一, 中村正斗, 伊藤文彰, 河村あさみ, 石崎明
    • Organizer
      日本農芸化学会北海道支部夏期シンポジウム
    • Related Report
      2011 Final Research Report
  • [Presentation] 泌乳持続性向上に関するウシ乳腺の細胞生物学的解析-ラクトフェリンが乳腺を構成する細胞の増殖に及ぼす影響2011

    • Author(s)
      中島恵一, 中村正斗, 伊藤文彰
    • Organizer
      平成23年度栄養生理研究会
    • Related Report
      2011 Final Research Report
  • [Presentation] ラクトフェリンによる血管新生促進因子の発現誘導2011

    • Author(s)
      中島恵一, 中村正斗, 河村あさみ, 石崎明
    • Organizer
      平成22年度日本酪農科学シンポジウム
    • Related Report
      2011 Final Research Report
  • [Presentation] ウシ乳腺上皮細胞の血管内皮増殖因子(VEGF)発現誘導におけるMAPキナーゼの関与2011

    • Author(s)
      中島恵一, 中村正斗, 伊藤文彰, 小酒井貴晴
    • Organizer
      日本畜産学会第112回大会
    • Related Report
      2011 Final Research Report
  • [Presentation] 子牛に対するビタミンE剤の投与が末梢血好中球のα-トコフェロール濃度に及ぼす影響2011

    • Author(s)
      樋口豪紀、北川詠子、間狩明美、吉田一万、郡和博、及川伸、永幡肇
    • Organizer
      第152回日本獣医学会学術集会集会
    • Related Report
      2011 Final Research Report
  • [Presentation] 細菌の遺伝子同定から乳房炎を防除する(特にマイコプラズマ性乳房炎について)2010

    • Author(s)
      樋口豪紀
    • Organizer
      日本乳房炎研究会
    • Related Report
      2011 Final Research Report
  • [Presentation] 黄色ブドウ球菌に対する特異的ファージを用いたウシ乳房炎治療応用への可能性2010

    • Author(s)
      岩野英知、樋口豪紀、井上博紀、萩原克郎、横田博、永幡肇、田村豊
    • Organizer
      日本乳房炎研究会
    • Related Report
      2011 Final Research Report
  • [Presentation] 牛乳房炎ファージセラピーに向けた黄色ブドウ球菌の凝集性評価2010

    • Author(s)
      丹治保典、谷夏織、栗本実樹、宮永一彦、樋口豪紀、岩野英知、井上博紀、永幡肇
    • Organizer
      日本乳房炎研究会
    • Related Report
      2011 Final Research Report
  • [Presentation] 黄色ブドウ球菌の乳腺感染モデルマウスに対するファージセラピーの検討2010

    • Author(s)
      岩野英知、樋口豪紀、井上博紀、井上裕介、高砂拓志、萩原克郎、丹治保典、永幡肇、横田博
    • Organizer
      第149回日本獣医学会学術集会
    • Related Report
      2011 Final Research Report
  • [Presentation] 黄色ブドウ球菌感染症へのファージ療法の応用2010

    • Author(s)
      岩野英知、樋口豪紀、井上博紀、横田博、永幡肇、小倉俊樹、近藤明宏、丹治保典、田村豊
    • Organizer
      動物用ワクチン-バイオ医薬品研究会
    • Related Report
      2011 Final Research Report
  • [Presentation] 牛マイコプラズマ性乳房炎の迅速簡易診断技術の開発とその応用に関する研究2010

    • Author(s)
      樋口豪紀・岩野英知・伊藤暢彦・河合一洋・草場信之・大林哲・廣瀬和彦・横田博・永幡肇
    • Organizer
      日本獣医師会
    • Related Report
      2011 Final Research Report
  • [Presentation] 子牛のマイコプラズマ性中耳炎に対する抗菌剤による予防対策の検討

    • Author(s)
      谷美穂、安藤貴朗、樋口豪紀、小岩政照
    • Organizer
      北海道獣医師会
    • Related Report
      2011 Final Research Report
  • [Presentation] ラクトフェリンが乳腺上皮細胞と乳腺線維芽細胞の増殖に及ぼす影響

    • Author(s)
      中島恵一, 中村正斗, 河村あさみ, 伊藤文彰
    • Organizer
      日本畜産学会第114回大会
    • Related Report
      2011 Final Research Report
  • [Presentation] ウシ乳房炎由来黄色ブドウ球菌に対するバクテリオファージの有効性

    • Author(s)
      井上裕介、岩野英知、樋口豪紀、井上博紀、高砂拓志、萩原克郎、丹治保典、永幡肇、田村豊、横田博
    • Organizer
      第150回日本獣医学会学術集会
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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