Study on development of sugar feeding method that prevents animal botulism

• Project/Area Number: 21580383
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: NIWA Koichi 東京農業大学, 生物産業学部, 教授 (20301012)
• Co-Investigator(Renkei-kenkyūsha): OHYAMA Tohru 東京農業大学, 生物産業学部, 教授 (60318178) ; WATANABE Toshihiro 東京農業大学, 生物産業学部, 教授 (80175695) ; OGURI Suguru 東京農業大学, 生物産業学部, 教授 (70277250)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ボツリヌス症 / ボツリヌス毒素 / 小腸上皮細胞 / 糖鎖 / 吸収 / 疾病予防 / ボツリヌス毒素複合体 / ボツリヌス中毒 / トランスサイトーシス / 感染症 / 病態生理学

Research Abstract

To establish the preventive against bovine botulism, the effects of sugar on the transport of botulinum toxin complex(L-TC) via cultured intestinal epithelial cells were examined. L-TC permeated the cell monolayers, and the permeation were potently inhibited by sialic acid in the cell culture medium, indicating that the binding of botulinum toxins to cell surface sialic acid leads to their transcytosis through intestinal epithelial cells. Present results imply that bovine botulism can be prevented by an addition of sialic acid to feed

Research Products

• [Journal Article] Characterization of sugar recognition by the toxin complex produced by Clostridium botulinum serotype C variant strain Yoichi (2011)
  • Author(s): Matsuo, T., Miyata, K., Inui, K., Ito, H., Horiuchi, R., Suzuki, T., Yoneyama, T., Oguma, K., Niwa, K., Watanabe, T., Ohyama, T.
  • Journal Title: FEMS Immunology Medicul Microbiology Volume: 63 Issue: 1 Pages: 35-43
  • DOI: 10.1111/j.1574-695x.2011.00825.x
  • Peer Reviewed
• [Journal Article] HA-33 facilitates transport of the serotype D botulinum toxin across a rat intestinal epithelial cell monolayer (2011)
  • Author(s): Ito, H., Sagane, Y., Miyata, K., Inui, K., Matsuo, T., Horiuchi, R., Ikeda, T., Suzuki, T., Hasegawa, K., Kouguchi, K., Oguma, K., Niwa, K., Ohyama, T., Watanabe T.
  • Journal Title: FEMS Immunology Medicul Microbiology Volume: 61 Issue: 3 Pages: 323-331
  • DOI: 10.1111/j.1574-695x.2011.00779.x
  • Peer Reviewed
• [Journal Article] Involvement of sialic acid in transport of serotype C1 botulinum toxins through rat intestinal cells (2010)
  • Author(s): Inui, K., Ito, H., Miyata, K., Matsuo, T., Horiuchi, R., Ikeda, T., Watanabe, T., Ohyanma, T. and Niwa, K.
  • Journal Title: J. Vet. Med. Sci Volume: 72 Pages: 1251-1255
  • URL: http://dx.doi.org/10.1292/jvms.10-0090
  • Peer Reviewed
• [Journal Article] Sialic acid-dependent binding and transcytosis of serotype D botulinum neurotoxin and toxin complex in rat intestinal epithelial cells (2010)
  • Author(s): Niwa, K., Yoneyama, T., Ito, H., Taira, M., Chikai, T., Kouguchi, H., Suzuki, T., Hasegawa, K., Miyata, K., Inui, K., Ikeda, T., Watanabe, T. and Ohyama, T.
  • Journal Title: Vet. Microbiol Volume: 141 Pages: 312-320
  • Peer Reviewed
• [Journal Article] Involvement of sialic acid in transport of serotype C1 botulinum toxins through rat intestinal epithelial cells (2010)
  • Author(s): Ken Inui
  • Journal Title: Journal of Veterinary Medical Science Volume: 72 Pages: 1251-1255
  • Peer Reviewed
• [Journal Article] Sialic acid-dependent binding and transcytosis of serotype D botulinum neurotoxin and toxin comnlex in rat intestinal epithelial cells. (2010)
  • Author(s): 丹羽光一
  • Journal Title: Veterinary Microbiology 141 Pages: 312-320
  • Peer Reviewed
• [Presentation] D型ボツリヌス毒素複合体の小腸上皮細胞層透過経路に関する研究 (2011)
  • Author(s): 吉田悠真
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] D型ボツリヌス毒素複合体の小腸上皮細胞層透過経路に関する研究 (2011)
  • Author(s): 吉田悠真
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 2011-09-24
• [Presentation] C型ボツリヌス毒素の小腸上皮細胞からの侵入機構の解析 (2010)
  • Author(s): 犬井健
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] C型ボツリヌス毒素の小腸上皮細胞からの侵入機構の解析 (2010)
  • Author(s): 犬井健
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 2010-12-08
• [Presentation] C型およびD型ボツリヌス毒素の小腸上皮細胞の透過におけるシアル酸の役割 (2010)
  • Author(s): 丹羽光一
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広
  • Year and Date: 2010-09-17
• [Presentation] C型およびD型ボツリヌス毒素の小腸上皮細胞の透過におけるシアル酸の役割 (2010)
  • Author(s): 丹羽光一
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広畜産大学(帯広)
  • Year and Date: 2010-09-17
• [Presentation] Clostridium botulinumの産生するC型ボツリヌス毒素の小腸上皮細胞透過機構の解析 (2010)
  • Author(s): 犬井健
  • Organizer: 第78回日本細菌学会北海道支部会総会
  • Place of Presentation: 江別
  • Year and Date: 2010-09-04
• [Presentation] Clostridium botulinumの産生するC型ボツリヌス毒素の小腸上皮細胞透過機構の解析 (2010)
  • Author(s): 犬井健
  • Organizer: 第78回日本細菌学会北海道支部総会
  • Place of Presentation: 酪農学園大学(江別)
  • Year and Date: 2010-09-04
• [Presentation] 世界最強・ボツリヌス毒素の構造と体内侵入戦略 (2010)
  • Author(s): 丹羽光一
  • Organizer: 第78回日本細菌学会北海道支部総会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2010-09-03
• [Presentation] Role of HA components of botulinum toxin complex in absorption from intestinal epithelial cells (2010)
  • Author(s): 伊藤博章
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-27
• [Presentation] Role of HA components of botulinum toxin complex in absorption from intestinal epithelial cells. (2010)
  • Author(s): 伊藤博章
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2010-03-27
• [Presentation] ボツリヌス毒素複合体の腸管上皮細胞への結合と透過における血球凝集素成分の役割 (2009)
  • Author(s): 伊藤博章
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] ボツリヌス毒素複合体の腸管上皮細胞への結合と透過における血球凝集素成分の役割 (2009)
  • Author(s): 伊藤博章
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 2009-10-24
• [Presentation] D型ボツリヌス毒素複合体の腸管上皮細胞への結合および透過におけるHA-33の役割 (2009)
  • Author(s): 伊藤博章
  • Organizer: 第77回日本細菌学会北海道支部学術総会
  • Place of Presentation: 札幌
  • Year and Date: 2009-09-18
• [Presentation] D型ボツリヌス毒素複合体の腸管上皮細胞への結合および透過におけるHA-33の役割 (2009)
  • Author(s): 伊藤博章
  • Organizer: 第77回日本細菌学会北海道支部学術総会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2009-09-18
• [Remarks]
• [Remarks]
  • URL: http://www.bioindustry.nodai.ac.jp/~seika/
• [Remarks]
  • URL: http://www.bioindustry.nodai.ac.jp/~seika/
• [Remarks]
  • URL: http://www.bioindustry.nodai.ac.jp/~seika/