Study on development of sugar feeding method that prevents animal botulism
Project/Area Number |
21580383
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied veterinary science
|
Research Institution | Tokyo University of Agriculture |
Principal Investigator |
NIWA Koichi 東京農業大学, 生物産業学部, 教授 (20301012)
|
Co-Investigator(Renkei-kenkyūsha) |
OHYAMA Tohru 東京農業大学, 生物産業学部, 教授 (60318178)
WATANABE Toshihiro 東京農業大学, 生物産業学部, 教授 (80175695)
OGURI Suguru 東京農業大学, 生物産業学部, 教授 (70277250)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | ボツリヌス症 / ボツリヌス毒素 / 小腸上皮細胞 / 糖鎖 / 吸収 / 疾病予防 / ボツリヌス毒素複合体 / ボツリヌス中毒 / トランスサイトーシス / 感染症 / 病態生理学 |
Research Abstract |
To establish the preventive against bovine botulism, the effects of sugar on the transport of botulinum toxin complex(L-TC) via cultured intestinal epithelial cells were examined. L-TC permeated the cell monolayers, and the permeation were potently inhibited by sialic acid in the cell culture medium, indicating that the binding of botulinum toxins to cell surface sialic acid leads to their transcytosis through intestinal epithelial cells. Present results imply that bovine botulism can be prevented by an addition of sialic acid to feed
|
Report
(4 results)
Research Products
(25 results)
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[Journal Article] Characterization of sugar recognition by the toxin complex produced by Clostridium botulinum serotype C variant strain Yoichi2011
Author(s)
Matsuo, T., Miyata, K., Inui, K., Ito, H., Horiuchi, R., Suzuki, T., Yoneyama, T., Oguma, K., Niwa, K., Watanabe, T., Ohyama, T.
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Journal Title
FEMS Immunology Medicul Microbiology
Volume: 63
Issue: 1
Pages: 35-43
DOI
Related Report
Peer Reviewed
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[Journal Article] HA-33 facilitates transport of the serotype D botulinum toxin across a rat intestinal epithelial cell monolayer2011
Author(s)
Ito, H., Sagane, Y., Miyata, K., Inui, K., Matsuo, T., Horiuchi, R., Ikeda, T., Suzuki, T., Hasegawa, K., Kouguchi, K., Oguma, K., Niwa, K., Ohyama, T., Watanabe T.
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Journal Title
FEMS Immunology Medicul Microbiology
Volume: 61
Issue: 3
Pages: 323-331
DOI
Related Report
Peer Reviewed
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[Journal Article] Involvement of sialic acid in transport of serotype C1 botulinum toxins through rat intestinal cells2010
Author(s)
Inui, K., Ito, H., Miyata, K., Matsuo, T., Horiuchi, R., Ikeda, T., Watanabe, T., Ohyanma, T. and Niwa, K.
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Journal Title
J. Vet. Med. Sci
Volume: 72
Pages: 1251-1255
URL
Related Report
Peer Reviewed
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[Journal Article] Sialic acid-dependent binding and transcytosis of serotype D botulinum neurotoxin and toxin complex in rat intestinal epithelial cells2010
Author(s)
Niwa, K., Yoneyama, T., Ito, H., Taira, M., Chikai, T., Kouguchi, H., Suzuki, T., Hasegawa, K., Miyata, K., Inui, K., Ikeda, T., Watanabe, T. and Ohyama, T.
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Journal Title
Vet. Microbiol
Volume: 141
Pages: 312-320
Related Report
Peer Reviewed
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