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Development of Neutralizing Antibodies against Staphylococcal Exfoliative Toxins

Research Project

Project/Area Number 21580387
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Clinical veterinary science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

NISHIFUJI Koji  東京農工大学, 大学院・農学研究院, 准教授 (20365422)

Co-Investigator(Kenkyū-buntansha) IWASAKI Toshiroh  東京農工大学, 大学院・農学研究院, 教授 (50262754)
Co-Investigator(Renkei-kenkyūsha) SUGAI Motoyuki  広島大学, 医歯(薬)学総合研究科, 教授 (10201568)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords感染症 / ブドウ球菌 / 表皮剥脱毒素 / デスモグレイン / 皮膚 / 膿痂疹 / ブドウ球菌性熱傷様皮膚症候群 / ブタ滲出性表皮炎 / 細胞接着 / 滲出性表皮炎
Research Abstract

In this project, we revealed that amino acid motifs critical for substrate specificity of Staphylococcus aureus exfoliative toxin B(ETB) may be located between amino acid residues of 65-232. This project also revealed that two S. pseudintermedius ETs selectively digest canine desmoglein 1, a cell-cell adhesion molecule in canine epidermis.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (27 results)

All 2012 2011 2010 2009 Other

All Journal Article (12 results) (of which Peer Reviewed: 11 results) Presentation (11 results) Remarks (4 results)

  • [Journal Article] Staphylococcus pseudintermedius exfoliative toxin EXI selectively digests canine desmoglein 1 and causes subcorneal clefts in canine epidermis2011

    • Author(s)
      Iyori K, Futagawa-Saito K, Hisatsune J, Yamamoto M, Sekiguchi M, Ide K, Son WG, Olivry T, Sugai M, Fukuyasu T, Iwasaki T, Nishifuji K
    • Journal Title

      Vet Dermatol

      Volume: Vol.22, No.4 Issue: 4 Pages: 319-326

    • DOI

      10.1111/j.1365-3164.2011.00952.x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Alteration of stratum corneum ceramide profiles in spontaneous canine model of atopic dermatitis2011

    • Author(s)
      Yoon JS, et al
    • Journal Title

      Exp Dermatol

      Volume: 20 Issue: 9 Pages: 732-736

    • DOI

      10.1111/j.1600-0625.2011.01306.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs2010

    • Author(s)
      Iyori K, Hisatsune J, Kawakami T, Shibata S, Murayama N, Ide K, Nagata M, Fukata T, Iwasaki T, Oshima K, Hattori M, Sugai M, Nishifuji K
    • Journal Title

      FEMS Microbiol Lett

      Volume: Vol.312, No.2 Issue: 2 Pages: 169-175

    • DOI

      10.1111/j.1574-6968.2010.02113.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Removal of amino-terminal extracellular domains of desmoglein 1 by staphylococcal exfoliative toxin is sufficient to initiate epidermal blister formation2010

    • Author(s)
      Nishifuji K, Shimizu A, Ishiko A, Iwasaki T, Amagai M
    • Journal Title

      J Dermatol Sci

      Volume: Vol.59, No.3 Pages: 184-191

    • NAID

      10027473355

    • URL

      http://www.sciencedirect.com/science/article/pii/S092318111000232X

    • Related Report
      2011 Final Research Report
  • [Journal Article] Antimicrobial susceptibility and methicillin resistance in Staphylococcus pseudintermedius and Staphylococcus schleiferi subsp. coagulans isolated from dogs with pyoderma in Japan2010

    • Author(s)
      Kawakami T, Shibata S, Murayama N, Nagata M, Nishifuji K, Iwasaki T, Fukata T
    • Journal Title

      J Vet Med Sci

      Volume: Vol.72, No.12 Pages: 1615-1619

    • NAID

      130000307697

    • URL

      http://www.jstage.jst.go.jp/article/jvms/72/12/72_10-0172/_article

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression analysis of desmosomal components of the novel canine epidermal keratinocyte cell line(MSCEK)2010

    • Author(s)
      Ide K, Yamamoto W, Sato M, Yabuzoe A, Iwasaki T, Nishifuji K
    • Journal Title

      J Vet Med Sci

      Volume: Vol.72, No.11 Pages: 1479-82

    • NAID

      130000264190

    • URL

      http://www.jstage.jst.go.jp/article/jvms/72/11/72_10-0089/_article

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Removal of amino-terminal extracellular domains of desmoglein 1 by staphylococcal exfoliative toxin is sufficient to initiate epidermal blister formation2010

    • Author(s)
      Nishifuji K, et al.
    • Journal Title

      J Dermatol Sci

      Volume: 59 Pages: 184-191

    • NAID

      10027473355

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs2010

    • Author(s)
      Iyori K, et al.
    • Journal Title

      FEMS Microbiol Lett

      Volume: 312 Pages: 169-175

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expresson analysis of desmosomal components of the novel canine epidermal keratinocyte cell line (MSCEK)2010

    • Author(s)
      Ide K, et al.
    • Journal Title

      J Vet Med Sci

      Volume: 72 Pages: 1479-1482

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased transepidermal water loss and decreased ceramide content in the lesional and non-lesional skin of canines with atopic dermatitis2009

    • Author(s)
      Shimada K
    • Journal Title

      Vet Dermatol 20

      Pages: 541-546

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and isolation of highly proliferative canine hair follicle keratinocytes, enriched for bulge cells : promising candidates for epithelial stem cells2009

    • Author(s)
      Kobayashi T
    • Journal Title

      Vet Dermatol 20

      Pages: 338-346

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of an enzyme-linked immunosorbent assay for detection of circulating IgG autoantibodies against canine desmoglein 3 in dogs with pemphigus2009

    • Author(s)
      Nishifuji K
    • Journal Title

      Vet Dermatol 20

      Pages: 331-337

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 皮膚科二次診療施設で分離されたStaphylococcus pseudintermediusにおける3種の表皮剥脱毒素遺伝子の保有率および毒素活性の比較解析2012

    • Author(s)
      山本真紀子、久恒順三、伊從慶太、菅井基行、南毅生、西藤公司
    • Organizer
      第15回日本獣医皮膚科学会学術大会
    • Place of Presentation
      大宮
    • Year and Date
      2012-03-18
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 一次診療施設にて表在性膿皮症と診断された犬由来のStaphylococcus pseudintermediusにおける表皮剥脱毒素遺伝子の保有状況2012

    • Author(s)
      伊從慶太、豊田陽一、久恒順三、村山信雄、井手香織、永田雅彦、岩崎利郎、菅井基行、西藤公司
    • Organizer
      第15回日本獣医皮膚科学会学術大会
    • Year and Date
      2012-03-18
    • Related Report
      2011 Final Research Report
  • [Presentation] 犬膿皮症由来Staphylococcus pseudintermedius表皮剥脱毒素ExpB産生株の全ゲノム配列解析2012

    • Author(s)
      久恒順三、平川英樹、伊從慶太、西藤公司、大島健志郎、服部正平、菅井基行
    • Organizer
      第6回日本ゲノム微生物学会年会
    • Place of Presentation
      東京
    • Year and Date
      2012-03-10
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 犬の膿皮症における抗菌薬耐性菌の現状と考えられる対策2010

    • Author(s)
      西藤公司
    • Organizer
      第31回動物臨床医学会年次大会
    • Place of Presentation
      大阪
    • Year and Date
      2010-11-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Staphylococcus pseudintermedius exfoliative toxins selectively digest canine desmoglein 1 and cause intraepidermal blisters in canine skin2010

    • Author(s)
      Iyori K, Futagawa-Saito K, Hisatsune J, Yamamoto M, Sekiguchi M, Ide K, Iwasaki T, Sugai M, Fukuyasu T, Nishifuji K
    • Organizer
      North American Veterinary Dermatology Forum 2010
    • Place of Presentation
      Portland, USA
    • Year and Date
      2010-04-15
    • Related Report
      2011 Final Research Report
  • [Presentation] Staphylococcus pseudintermedius exfoliative toxins selectively digest canine desmoglein 1 and cause intraepidermal blisters in canine skin2010

    • Author(s)
      Iyori K, et al.
    • Organizer
      North American Veterinary Dermatology Forum 2010
    • Place of Presentation
      米国・ポートランド
    • Year and Date
      2010-04-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 膿皮症由来Staphylococcus pseudintermediusにおける多剤耐性およびメチシリン耐性状況2010

    • Author(s)
      川上哲司、柴田早苗、林玲子、深町綾、村山信雄、永田雅彦、西藤公司、岩崎利郎、深田恒夫
    • Organizer
      第13回日本獣医皮膚科学会学術大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 深在性膿皮症2010

    • Author(s)
      西藤公司
    • Organizer
      JSVIM/JSVCP2010年大会
    • Place of Presentation
      東京
    • Year and Date
      2010-02-13
    • Related Report
      2011 Final Research Report
  • [Presentation] 膿皮症の病態・診断・治療.平成21年度日本獣医師会2010

    • Author(s)
      西藤公司
    • Organizer
      学会年次大会
    • Place of Presentation
      宮崎
    • Year and Date
      2010-01-29
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of an in vitro culture system to analyze ceramide metabolism in canine skin2009

    • Author(s)
      Yoon JS
    • Organizer
      23^<rd> Annual Congress of the ESVD-ECVD on Veterinary Dermatology
    • Place of Presentation
      スロベニア国ブレッド市
    • Year and Date
      2009-09-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] 膿皮症のイヌから分離したStaphylococcus intermedius groupの抗菌薬耐性保有率2009

    • Author(s)
      川上哲司、野並崇志、林玲子、柴田早苗、村山信雄、永田雅彦、西藤公司、岩崎利郎、深田恒夫
    • Organizer
      第147回日本獣医学会学術集会
    • Place of Presentation
      宇都宮
    • Year and Date
      2009-04-02
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://kenkyu-web.tuat.ac.jp/Profiles/15/0001474/profile.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://kenkyu-web.tuat.ac.jp/Profiles/15/0001474/profile.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://kenkyu-web.tuat.ac.jp/Profiles/15/0001474/profile.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://kenkyu-web.tuat.ac.jp/Profiles/15/0001474/profile.html

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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