Development of Neutralizing Antibodies against Staphylococcal Exfoliative Toxins

• Project/Area Number: 21580387
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: NISHIFUJI Koji 東京農工大学, 大学院・農学研究院, 准教授 (20365422)
• Co-Investigator(Kenkyū-buntansha): IWASAKI Toshiroh 東京農工大学, 大学院・農学研究院, 教授 (50262754)
• Co-Investigator(Renkei-kenkyūsha): SUGAI Motoyuki 広島大学, 医歯(薬)学総合研究科, 教授 (10201568)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 感染症 / ブドウ球菌 / 表皮剥脱毒素 / デスモグレイン / 皮膚 / 膿痂疹 / ブドウ球菌性熱傷様皮膚症候群 / ブタ滲出性表皮炎 / 細胞接着 / 滲出性表皮炎

Research Abstract

In this project, we revealed that amino acid motifs critical for substrate specificity of Staphylococcus aureus exfoliative toxin B(ETB) may be located between amino acid residues of 65-232. This project also revealed that two S. pseudintermedius ETs selectively digest canine desmoglein 1, a cell-cell adhesion molecule in canine epidermis.

Research Products

• [Journal Article] Staphylococcus pseudintermedius exfoliative toxin EXI selectively digests canine desmoglein 1 and causes subcorneal clefts in canine epidermis (2011)
  • Author(s): Iyori K, Futagawa-Saito K, Hisatsune J, Yamamoto M, Sekiguchi M, Ide K, Son WG, Olivry T, Sugai M, Fukuyasu T, Iwasaki T, Nishifuji K
  • Journal Title: Vet Dermatol Volume: Vol.22, No.4 Issue: 4 Pages: 319-326
  • DOI: 10.1111/j.1365-3164.2011.00952.x
  • Peer Reviewed
• [Journal Article] Alteration of stratum corneum ceramide profiles in spontaneous canine model of atopic dermatitis (2011)
  • Author(s): Yoon JS, et al
  • Journal Title: Exp Dermatol Volume: 20 Issue: 9 Pages: 732-736
  • DOI: 10.1111/j.1600-0625.2011.01306.x
  • Peer Reviewed
• [Journal Article] Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs (2010)
  • Author(s): Iyori K, Hisatsune J, Kawakami T, Shibata S, Murayama N, Ide K, Nagata M, Fukata T, Iwasaki T, Oshima K, Hattori M, Sugai M, Nishifuji K
  • Journal Title: FEMS Microbiol Lett Volume: Vol.312, No.2 Issue: 2 Pages: 169-175
  • DOI: 10.1111/j.1574-6968.2010.02113.x
  • Peer Reviewed
• [Journal Article] Removal of amino-terminal extracellular domains of desmoglein 1 by staphylococcal exfoliative toxin is sufficient to initiate epidermal blister formation (2010)
  • Author(s): Nishifuji K, Shimizu A, Ishiko A, Iwasaki T, Amagai M
  • Journal Title: J Dermatol Sci Volume: Vol.59, No.3 Pages: 184-191
  • NAID: 10027473355
  • URL: http://www.sciencedirect.com/science/article/pii/S092318111000232X
• [Journal Article] Antimicrobial susceptibility and methicillin resistance in Staphylococcus pseudintermedius and Staphylococcus schleiferi subsp. coagulans isolated from dogs with pyoderma in Japan (2010)
  • Author(s): Kawakami T, Shibata S, Murayama N, Nagata M, Nishifuji K, Iwasaki T, Fukata T
  • Journal Title: J Vet Med Sci Volume: Vol.72, No.12 Pages: 1615-1619
  • NAID: 130000307697
  • URL: http://www.jstage.jst.go.jp/article/jvms/72/12/72_10-0172/_article
  • Peer Reviewed
• [Journal Article] Expression analysis of desmosomal components of the novel canine epidermal keratinocyte cell line(MSCEK) (2010)
  • Author(s): Ide K, Yamamoto W, Sato M, Yabuzoe A, Iwasaki T, Nishifuji K
  • Journal Title: J Vet Med Sci Volume: Vol.72, No.11 Pages: 1479-82
  • NAID: 130000264190
  • URL: http://www.jstage.jst.go.jp/article/jvms/72/11/72_10-0089/_article
  • Peer Reviewed
• [Journal Article] Removal of amino-terminal extracellular domains of desmoglein 1 by staphylococcal exfoliative toxin is sufficient to initiate epidermal blister formation (2010)
  • Author(s): Nishifuji K, et al.
  • Journal Title: J Dermatol Sci Volume: 59 Pages: 184-191
  • NAID: 10027473355
  • Peer Reviewed
• [Journal Article] Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs (2010)
  • Author(s): Iyori K, et al.
  • Journal Title: FEMS Microbiol Lett Volume: 312 Pages: 169-175
  • Peer Reviewed
• [Journal Article] Expresson analysis of desmosomal components of the novel canine epidermal keratinocyte cell line (MSCEK) (2010)
  • Author(s): Ide K, et al.
  • Journal Title: J Vet Med Sci Volume: 72 Pages: 1479-1482
  • Peer Reviewed
• [Journal Article] Increased transepidermal water loss and decreased ceramide content in the lesional and non-lesional skin of canines with atopic dermatitis (2009)
  • Author(s): Shimada K
  • Journal Title: Vet Dermatol 20 Pages: 541-546
  • Peer Reviewed
• [Journal Article] Identification and isolation of highly proliferative canine hair follicle keratinocytes, enriched for bulge cells : promising candidates for epithelial stem cells (2009)
  • Author(s): Kobayashi T
  • Journal Title: Vet Dermatol 20 Pages: 338-346
  • Peer Reviewed
• [Journal Article] Development of an enzyme-linked immunosorbent assay for detection of circulating IgG autoantibodies against canine desmoglein 3 in dogs with pemphigus (2009)
  • Author(s): Nishifuji K
  • Journal Title: Vet Dermatol 20 Pages: 331-337
  • Peer Reviewed
• [Presentation] 皮膚科二次診療施設で分離されたStaphylococcus pseudintermediusにおける3種の表皮剥脱毒素遺伝子の保有率および毒素活性の比較解析 (2012)
  • Author(s): 山本真紀子、久恒順三、伊從慶太、菅井基行、南毅生、西藤公司
  • Organizer: 第15回日本獣医皮膚科学会学術大会
  • Place of Presentation: 大宮
  • Year and Date: 2012-03-18
• [Presentation] 一次診療施設にて表在性膿皮症と診断された犬由来のStaphylococcus pseudintermediusにおける表皮剥脱毒素遺伝子の保有状況 (2012)
  • Author(s): 伊從慶太、豊田陽一、久恒順三、村山信雄、井手香織、永田雅彦、岩崎利郎、菅井基行、西藤公司
  • Organizer: 第15回日本獣医皮膚科学会学術大会
  • Year and Date: 2012-03-18
• [Presentation] 犬膿皮症由来Staphylococcus pseudintermedius表皮剥脱毒素ExpB産生株の全ゲノム配列解析 (2012)
  • Author(s): 久恒順三、平川英樹、伊從慶太、西藤公司、大島健志郎、服部正平、菅井基行
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 東京
  • Year and Date: 2012-03-10
• [Presentation] 犬の膿皮症における抗菌薬耐性菌の現状と考えられる対策 (2010)
  • Author(s): 西藤公司
  • Organizer: 第31回動物臨床医学会年次大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-11-20
• [Presentation] Staphylococcus pseudintermedius exfoliative toxins selectively digest canine desmoglein 1 and cause intraepidermal blisters in canine skin (2010)
  • Author(s): Iyori K, Futagawa-Saito K, Hisatsune J, Yamamoto M, Sekiguchi M, Ide K, Iwasaki T, Sugai M, Fukuyasu T, Nishifuji K
  • Organizer: North American Veterinary Dermatology Forum 2010
  • Place of Presentation: Portland, USA
  • Year and Date: 2010-04-15
• [Presentation] Staphylococcus pseudintermedius exfoliative toxins selectively digest canine desmoglein 1 and cause intraepidermal blisters in canine skin (2010)
  • Author(s): Iyori K, et al.
  • Organizer: North American Veterinary Dermatology Forum 2010
  • Place of Presentation: 米国・ポートランド
  • Year and Date: 2010-04-15
• [Presentation] 膿皮症由来Staphylococcus pseudintermediusにおける多剤耐性およびメチシリン耐性状況 (2010)
  • Author(s): 川上哲司、柴田早苗、林玲子、深町綾、村山信雄、永田雅彦、西藤公司、岩崎利郎、深田恒夫
  • Organizer: 第13回日本獣医皮膚科学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-14
• [Presentation] 深在性膿皮症 (2010)
  • Author(s): 西藤公司
  • Organizer: JSVIM/JSVCP2010年大会
  • Place of Presentation: 東京
  • Year and Date: 2010-02-13
• [Presentation] 膿皮症の病態・診断・治療.平成21年度日本獣医師会 (2010)
  • Author(s): 西藤公司
  • Organizer: 学会年次大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-01-29
• [Presentation] Development of an in vitro culture system to analyze ceramide metabolism in canine skin (2009)
  • Author(s): Yoon JS
  • Organizer: 23^<rd> Annual Congress of the ESVD-ECVD on Veterinary Dermatology
  • Place of Presentation: スロベニア国ブレッド市
  • Year and Date: 2009-09-18
• [Presentation] 膿皮症のイヌから分離したStaphylococcus intermedius groupの抗菌薬耐性保有率 (2009)
  • Author(s): 川上哲司、野並崇志、林玲子、柴田早苗、村山信雄、永田雅彦、西藤公司、岩崎利郎、深田恒夫
  • Organizer: 第147回日本獣医学会学術集会
  • Place of Presentation: 宇都宮
  • Year and Date: 2009-04-02
• [Remarks]
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• [Remarks]
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• [Remarks]
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• [Remarks]
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