Development of clinical therapeutic methods against failure of fertilizing functions causing summer infertility in boars

• Project/Area Number: 21580389
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Gifu University
• Principal Investigator: MURASE Tetsuma 岐阜大学, 応用生物科学部, 教授 (30303514)
• Co-Investigator(Kenkyū-buntansha): HARAYAMA Hiroshi 神戸大学, 農学研究科, 准教授 (30281140) ; MIYAKE Masashi 神戸大学, 自然科学系先端融合研究環, 教授 (60093316)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: 臨床繁殖 / 産科精子 / ブタ / 夏季不妊 / 精子 / 受精 / 先体反応 / ハイパーアクチベーション / 希釈液 / シクロオキシゲナーゼ / 夏季不妊症 / 臨床 / イオノホア

Research Abstract

In an attempt to improving boar sperm quality which decreases in summer and stable provision with semen leading to stable production of pork during the year, this study investigated a new diluent for boar semen storage, a new method to evaluate frozen-thawed bull sperm quality and development of a method to improve fertilizing ability of frozen-thawed bull spermatozoa. Bull spermatozoa were used as a reference to boar spermatozoa. Modified diluents for boar semen improved conception rates. In bulls, new method for sperm evaluation was found out and modification of diluent for freezing bull semen enabled improvement of sperm fertilizing ability.

Research Products

• [Journal Article] Dilution ofboar ejaculates with BTScontaining HEPES in place ofbicarbonate immediately afterejaculation can reduce the increasedinducibility of the acrosome reactionby treatment with calcium and calciumionophore A23187, which ispotentially associated with (2010)
  • Author(s): Murase T, Imaeda N, Yamada H, TakasuM, Taguchi K, Katoh T
  • Journal Title: J. Reprod. Dev Volume: 56 Pages: 309-14
  • Peer Reviewed
• [Journal Article] Relationship of protein tyrosine phosphorylation state with toleranceto frozen storage and the potential to undergo cyclic AMP-dependent hyperactivation in the spermatozoa of Japanese Black bulls (2010)
  • Author(s): Harayama, H., Nishijima, K., Murase, T., Sakase, M., Fukushima, M
  • Journal Title: Mol. Reprod. Dev Volume: 77 Pages: 910-21
  • Peer Reviewed
• [Journal Article] Dilution of boar ejaculates with BTS containing HEPES in place of bicarbonate immediately after ejaculation can reduce the increased inducibility of the acrosome reaction bytreatment with calcium and calcium ionophore A23187, which is potentially associatedwith boar subfertility. (2010)
  • Author(s): Tetsuma MURASE
  • Journal Title: Journal of Reproduction and Development Volume: 56 Pages: 309-314
  • Peer Reviewed
• [Journal Article] Relationship of protein tyrosine phosphorylation state with tolerance to frozen storage and the potential to undergo cyclic AMP-dependent hyperactivation in the spermatozoa of Japanese Black bulls (2010)
  • Author(s): Hiroshi HARAYAMA
  • Journal Title: Molecular Reproduction and Development Volume: 77 Pages: 910-921
  • Peer Reviewed
• [Journal Article] Dilution of boar ejaculates with BTS containing HEPES in place of bicarbonate immediately after ejaculation can reduce the increased inducibility of the acrosome reaction by treatment with calcium and calcium ionophore A23187, which is potentially associated with boar subfertility (2010)
  • Author(s): Tetsuma MURASE
  • Journal Title: Journal of Reproduction and Development (In press)
  • NAID: 10026434144
  • Peer Reviewed
• [Presentation] Desalting of boar seminal plasma by gel filtration (2010)
  • Author(s): Murase, T., Imaeda, N., Yamada, H., Harayama, H., Takasu, M., Taguchi, K., and Katoh, T.
  • Organizer: 11th International Symposium on Spermatology
  • Place of Presentation: Okinawa
  • Year and Date: 2010-06-25
• [Presentation] Desalting of boar seminal plasma by gel filtration (2010)
  • Author(s): Tetsuma MURASE
  • Organizer: 11th International Symposium on Spermatology
  • Place of Presentation: 那覇
  • Year and Date: 2010-06-25