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Development of clinical therapeutic methods against failure of fertilizing functions causing summer infertility in boars

Research Project

Project/Area Number 21580389
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Clinical veterinary science
Research InstitutionGifu University

Principal Investigator

MURASE Tetsuma  岐阜大学, 応用生物科学部, 教授 (30303514)

Co-Investigator(Kenkyū-buntansha) HARAYAMA Hiroshi  神戸大学, 農学研究科, 准教授 (30281140)
MIYAKE Masashi  神戸大学, 自然科学系先端融合研究環, 教授 (60093316)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywords臨床繁殖 / 産科精子 / ブタ / 夏季不妊 / 精子 / 受精 / 先体反応 / ハイパーアクチベーション / 希釈液 / シクロオキシゲナーゼ / 夏季不妊症 / 臨床 / イオノホア
Research Abstract

In an attempt to improving boar sperm quality which decreases in summer and stable provision with semen leading to stable production of pork during the year, this study investigated a new diluent for boar semen storage, a new method to evaluate frozen-thawed bull sperm quality and development of a method to improve fertilizing ability of frozen-thawed bull spermatozoa. Bull spermatozoa were used as a reference to boar spermatozoa. Modified diluents for boar semen improved conception rates. In bulls, new method for sperm evaluation was found out and modification of diluent for freezing bull semen enabled improvement of sperm fertilizing ability.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (7 results)

All 2010

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (2 results)

  • [Journal Article] Dilution ofboar ejaculates with BTScontaining HEPES in place ofbicarbonate immediately afterejaculation can reduce the increasedinducibility of the acrosome reactionby treatment with calcium and calciumionophore A23187, which ispotentially associated with2010

    • Author(s)
      Murase T, Imaeda N, Yamada H, TakasuM, Taguchi K, Katoh T
    • Journal Title

      J. Reprod. Dev

      Volume: 56 Pages: 309-14

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Relationship of protein tyrosine phosphorylation state with toleranceto frozen storage and the potential to undergo cyclic AMP-dependent hyperactivation in the spermatozoa of Japanese Black bulls2010

    • Author(s)
      Harayama, H., Nishijima, K., Murase, T., Sakase, M., Fukushima, M
    • Journal Title

      Mol. Reprod. Dev

      Volume: 77 Pages: 910-21

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dilution of boar ejaculates with BTS containing HEPES in place of bicarbonate immediately after ejaculation can reduce the increased inducibility of the acrosome reaction bytreatment with calcium and calcium ionophore A23187, which is potentially associatedwith boar subfertility.2010

    • Author(s)
      Tetsuma MURASE
    • Journal Title

      Journal of Reproduction and Development

      Volume: 56 Pages: 309-314

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationship of protein tyrosine phosphorylation state with tolerance to frozen storage and the potential to undergo cyclic AMP-dependent hyperactivation in the spermatozoa of Japanese Black bulls2010

    • Author(s)
      Hiroshi HARAYAMA
    • Journal Title

      Molecular Reproduction and Development

      Volume: 77 Pages: 910-921

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dilution of boar ejaculates with BTS containing HEPES in place of bicarbonate immediately after ejaculation can reduce the increased inducibility of the acrosome reaction by treatment with calcium and calcium ionophore A23187, which is potentially associated with boar subfertility2010

    • Author(s)
      Tetsuma MURASE
    • Journal Title

      Journal of Reproduction and Development (In press)

    • NAID

      10026434144

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Desalting of boar seminal plasma by gel filtration2010

    • Author(s)
      Murase, T., Imaeda, N., Yamada, H., Harayama, H., Takasu, M., Taguchi, K., and Katoh, T.
    • Organizer
      11th International Symposium on Spermatology
    • Place of Presentation
      Okinawa
    • Year and Date
      2010-06-25
    • Related Report
      2011 Final Research Report
  • [Presentation] Desalting of boar seminal plasma by gel filtration2010

    • Author(s)
      Tetsuma MURASE
    • Organizer
      11th International Symposium on Spermatology
    • Place of Presentation
      那覇
    • Year and Date
      2010-06-25
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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