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Study on establishment of the dog frozen semen technique for the purpose of the frozen semen bank establishment of assistance dog

Research Project

Project/Area Number 21580396
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Clinical veterinary science
Research InstitutionNippon Veterinary and Life Science University

Principal Investigator

HORI Tatsuya  日本獣医生命科学大学, 獣医学部, 准教授 (80277665)

Co-Investigator(Kenkyū-buntansha) TSUTSUI Toshihiko  日本獣医生命科学大学, 獣医学部, 教授 (40060529)
KAWAKAMI Eiichi  日本獣医生命科学大学, 獣医学部, 教授 (80139352)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords臨床繁殖 / 産科 / 犬 / 凍結精液 / 高齢犬 / 精子 / 造精機能 / グリセリン濃度 / グリセリン平衡時間 / 加齢に伴う影響 / 低温保存 / 輸送 / 子宮内授精 / 精液希釈液 / 内視鏡
Research Abstract

It is the present situations in Japan that the assistance dog including the guide dog is very insufficient. Because the lineage is necessary for reproduction of these dogs, it will be necessary to introduce the semen of an excellent dog and breed systematically at earth level in future. The establishment of the canine frozen semen technique that practical use is possible is indispensable. Therefore, for the purpose of establishment of canine frozen semen bank, the development of frozen semen technique providing high conception rate by the vaginal insemination and the establishment of the nonsurgical artificial insemination technique to reduce the aggression on dog were studied.
As a result, Egg-yolk tris fructose citric acid extender was effective, and a high conception rate was provided by the surgical intrauterine insemination of canine frozen semen. In addition, it became clear that intrauterine insemination by the semen frozen after preservation at low temperature until 48 hours provided the high conception rate. It was thought that the semen frozen after transport of semen at low temperature from the distant place in Japan or the foreign countries might be useful. However, when we observed the vagina and the external os of uterus before and after the ovulation using the endoscope(fiberscope) for small animals, the insertion of the catheter was possible, but the passage of a catheter was able to insert in only one of 11 bitches. Therefore, I could not examine the conception by the transvaginal intrauterine insemination of canine frozen semen. It in future, it was thought that the expansion of the observable range of the external os of uterus, the development of an air pressure and the development of catheter that enabled it would be necessary.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (2 results)

All 2009 Other

All Presentation (2 results)

  • [Presentation] 犬射出精液における低温保存温度の検討2009

    • Author(s)
      堀達也
    • Organizer
      第148回日本獣医学会
    • Place of Presentation
      とりぎん文化会館(鳥取)
    • Year and Date
      2009-09-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] 犬射出精液における低温保存温度の検討

    • Author(s)
      堀達也・吉國竜太・佐藤立人・曽宮陽子・高木勝子・岸部友里恵・篠田翔子・河上栄一・筒井敏彦
    • Organizer
      第148回日本獣医学会
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2017-10-13  

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