• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a novel calorimetric method for analyzing microbial properties of soil including VBNC microbes.

Research Project

Project/Area Number 21580400
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Boundary agriculture
Research InstitutionMie University

Principal Investigator

TANAKA Akiyoshi  三重大学, 大学院・生物資源学研究科, 教授 (10155111)

Co-Investigator(Renkei-kenkyūsha) SENOO Keishi  東京大学, 農学生命科学研究科, 教授 (40206652)
Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords環境分析 / 熱測定 / 土壌微生物 / バイオログ法 / 機能多様性 / 微生物活性 / 微生物多様性 / 資化熱 / 微生物群落構造 / DGGE法 / 農薬
Research Abstract

A novel calorimetric method for analyzing functional properties of soil microbes including VBNC microbes was developed. Exothermic processes of carbon-source assimilation by soil microbes were directly observed using soil samples as they were, and calorimetric parameters which characterize the exothermic procedures were evaluated for each combination of soil sample and carbon source. It was shown that functional properties of soil microbes, such as resistibility of microbes against chemicals and functional diversity of soil microbes, can be quantitatively evaluated on the basis of the analysis of the parameters.

Report

(5 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (34 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (27 results) Book (1 results)

  • [Journal Article] 微生物熱測定データ解析モデルの妥当性の検討2010

    • Author(s)
      坂宮章世,田中晶善
    • Journal Title

      熱測定

      Volume: 37(2) Pages: 58-63

    • NAID

      10029867034

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Calorimetric Analysis of Effects of a Soil-Stress Compound on Soil-Microbial Activity2010

    • Author(s)
      Akiyo K. Sakamiya, Natsuki Origuchi, and Akiyoshi Tanaka
    • Journal Title

      Netsusokutei (Calorimetry and Thermal Analysis)

      Volume: 37(5) Pages: 186-190

    • NAID

      10029867317

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Calorimetric Analysis of Effects of a Soil-Stress Compound on Soil-Microbial Activity2010

    • Author(s)
      Akiyo K.Sakamiya, Natsuki Origuchi, Akiyoshi Tanaka
    • Journal Title

      Netsusokutei (Calorimetry and Thermal Analysis)

      Volume: 37 Pages: 186-190

    • NAID

      10029867317

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 微生物熱測定データ解析モデルの妥当性の検討2010

    • Author(s)
      坂宮章世, 田中晶善
    • Journal Title

      熱測定 37

      Pages: 58-63

    • NAID

      10029867034

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kinetically trapped metastable intermediate of a disulfidedeficient mutant of the starch-binding domain of glucoamylase2009

    • Author(s)
      Hayuki Sugimoto, Akiyoshi Tanaka, et al.
    • Journal Title

      Protein Science 18

      Pages: 1715-1723

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Family 17 and Family 28 Carbohydrate-Binding Modules from Clostridium josui Cel5A2009

    • Author(s)
      Yuko Araki, Akiyoshi Tanaka, et al.
    • Journal Title

      Biosci.Biotechnol.Biochem. 73

      Pages: 1028-1032

    • NAID

      10027540619

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 熱測定法とPCR-DGGE法を用いた土壌微生物群集の多様性評価2013

    • Author(s)
      石川卓、小野泰弘、妹尾啓史、田中晶善
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2013-03-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 熱測定法を用いた土壌微生物の資化活性に基づく土壌試料の保存条件検討2013

    • Author(s)
      石川卓、林寛人、妹尾啓史、田中晶善
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2013-03-27
    • Related Report
      2012 Final Research Report
  • [Presentation] Development of evaluation methods for soil microbial diversity by carbon source utilization activity using multiplex calorimetry2012

    • Author(s)
      T. Ishikawa, Y. Ono, K. Senoo, and A. Tanaka
    • Organizer
      15th International congress on Thermal Analysis and Calorimetry
    • Place of Presentation
      近畿大学(東大阪)
    • Year and Date
      2012-08-24
    • Related Report
      2012 Final Research Report
  • [Presentation] 熱測定法による土壌微生物の多様性評価2012

    • Author(s)
      小野泰弘、石川卓、妹尾啓史、田中晶善
    • Organizer
      日本土壌微生物学会
    • Place of Presentation
      神戸大学(神戸)
    • Year and Date
      2012-06-23
    • Related Report
      2012 Final Research Report
  • [Presentation] A Novel Non-Destructive System to Determine MicrobialI Growth Activity in Foods and Its Application to the Quantitative Study of Preservative Effect2011

    • Author(s)
      Aki K.Hada, Hisao Horiguchi, Yumiko Mishima, Akiyo K.Sakamiya, Katsutada Takahashi, Akiyoshi Tanaka
    • Organizer
      XIII International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      札幌
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 微生物活性測定を目的とした多試料等温型熱量計の試作とその応用2010

    • Author(s)
      畑田優、坂宮章世、高橋克忠、田中晶善
    • Organizer
      日本熱測定学会
    • Place of Presentation
      三重大学(津)
    • Year and Date
      2010-09-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 熱測定法による、土壌微生物活性への塩化ナトリウムの影響の解析2010

    • Author(s)
      坂宮章世、蒔田和弥、桑原麻衣、近藤沙紀、田中晶善
    • Organizer
      日本熱測定学会
    • Place of Presentation
      三重大学(津)
    • Year and Date
      2010-09-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 微生物活性測定を目的とした多試料等温型熱量計の試作とその応用2010

    • Author(s)
      畑田優、坂宮章世、高橋克忠、田中晶善
    • Organizer
      日本熱測定学会
    • Place of Presentation
      津(三重大学)
    • Year and Date
      2010-09-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] 疎水性低分子結合に伴うリポカリン型プロスタグランジンD合成酵素(L-PGDS)の熱安定性2010

    • Author(s)
      飯田津喜美、深田はるみ、宮本優也、田中晶善、裏出良博、乾隆
    • Organizer
      日本熱測定学会
    • Place of Presentation
      津(三重大学)
    • Year and Date
      2010-09-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] 熱測定法による、土壌微生物活性への塩化ナトリウムの影響の解析2010

    • Author(s)
      坂宮章世、蒔田和弥、桑原麻衣、近藤沙紀、田中晶善
    • Organizer
      日本熱測定学会
    • Place of Presentation
      津(三重大学)
    • Year and Date
      2010-09-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] タイプB,Cの糖質結合モジュールの結合特性2010

    • Author(s)
      荒木裕子、近藤誠、後藤正和、田中晶善、苅田修一
    • Organizer
      日本熱測定学会
    • Place of Presentation
      津(三重大学)
    • Year and Date
      2010-09-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] 多サンプル同時熱測定法による、各種土壌の微生物活性への除草剤の影響の解析2010

    • Author(s)
      坂宮章世、折口菜都希、田中晶善
    • Organizer
      日本土壌微生物学会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2010-05-21
    • Related Report
      2012 Final Research Report
  • [Presentation] 多サンプル同時熱測定法による土壌の生物性評価の試み-各種炭素化合物代謝過程の解析に基づいて-2010

    • Author(s)
      坂宮章世、妹尾啓史、田中晶善
    • Organizer
      日本土壌微生物学会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2010-05-21
    • Related Report
      2012 Final Research Report
  • [Presentation] 多サンプル同時熱測定法による,各種土壌の微生物活性への除草剤の影響の解析2010

    • Author(s)
      坂宮章世、折口菜都希、田中晶善
    • Organizer
      日本土壌微生物学会
    • Place of Presentation
      東京(東京大学)
    • Year and Date
      2010-05-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 多サンプル同時熱測定法による土壌の生物性評価の試み-各種炭素化合物代謝過程の解析に基づいて-2010

    • Author(s)
      坂宮章世、妹尾啓史、田中晶善
    • Organizer
      日本土壌微生物学会
    • Place of Presentation
      東京(東京大学)
    • Year and Date
      2010-05-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 疎水性低分子結合に伴うマウスリポカリン型プロスタグランジンD合成酵素の安定化2009

    • Author(s)
      飯田津喜美, 田中晶善, ら
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸ポートアイランド (神戸市)
    • Year and Date
      2009-10-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] 微生物熱測定データ解析モデルの検討2009

    • Author(s)
      坂宮章世, 田中晶善
    • Organizer
      熱測定討論会 (日本熱測定学会)
    • Place of Presentation
      首都大学東京 (八王子市)
    • Year and Date
      2009-09-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] 熱測定法による, 土壌微生物活性への土壌ストレス物質の影響の解析2009

    • Author(s)
      坂宮章世, 折口菜都希, 田中晶善
    • Organizer
      熱測定討論会 (日本熱測定学会)
    • Place of Presentation
      首都大学東京 (八王子市)
    • Year and Date
      2009-09-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] 微生物熱測定データ解析モデルの検討2009

    • Author(s)
      坂宮章世、田中晶善
    • Organizer
      日本熱測定学会
    • Place of Presentation
      首都大学東京(八王子)
    • Year and Date
      2009-09-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 熱測定法による、土壌微生物活性への土壌ストレス物質の影響の解析2009

    • Author(s)
      坂宮章世、折口菜都希、田中晶善
    • Organizer
      日本熱測定学会
    • Place of Presentation
      首都大学東京(八王子)
    • Year and Date
      2009-09-28
    • Related Report
      2012 Final Research Report
  • [Presentation] グルコアミラーゼのデンプン結合ドメインにおける疎水性アミノ酸残基の役割2009

    • Author(s)
      増田佳史, 田中晶善, ら
    • Organizer
      熱測定討論会 (日本熱測定学会)
    • Place of Presentation
      首都大学東京 (八王子市)
    • Year and Date
      2009-09-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] グルコアミラーゼのデンプン結合ドメインにおける疎水性アミノ酸残基の役割2009

    • Author(s)
      増田佳史, 田中晶善, ら
    • Organizer
      日本農芸化学会 (関西支部例会)
    • Place of Presentation
      大阪府立大学 (堺市)
    • Year and Date
      2009-07-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒトリポカリン型プロスタグランジンD 合成酵素の熱変性過程の解析2009

    • Author(s)
      飯田津喜美, 田中晶善, ら
    • Organizer
      日本タンパク質学会
    • Place of Presentation
      熊本全日空ホテルニュースカイ (熊本市)
    • Year and Date
      2009-05-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] 熱測定法とPCR-DGGE法を用いた土壌微生物群集の多様性評価

    • Author(s)
      石川卓、小野泰弘、妹尾啓史、田中晶善
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東北大学(仙台)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 熱測定法を用いた土壌微生物の資化活性に基づく土壌試料の保存条件検討

    • Author(s)
      石川卓、林寛人、妹尾啓史、田中晶善
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東北大学(仙台)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of evaluation methods for soil microbial diversity by carbon source utilization activity using multiplex calorimetry

    • Author(s)
      T. Ishikawa, Y. Ono, K. Senoo, and A. Tanaka
    • Organizer
      International congress on Thermal Analysis and Calorimetry
    • Place of Presentation
      近畿大学(東大阪)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 熱測定法による土壌微生物の多様性評価

    • Author(s)
      小野泰弘、石川卓、妹尾啓史、田中晶善
    • Organizer
      土壌微生物学会
    • Place of Presentation
      神戸大学(神戸)
    • Related Report
      2012 Annual Research Report
  • [Book] 熱量測定・熱分析ハンドブック第2版「ジスルフィド結合と蛋白質の安定性」2010

    • Author(s)
      田中晶善
    • Total Pages
      1
    • Publisher
      丸善 (株) 出版事業部
    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi