Studies on a novel protein promoting cell self-renewal and translation for the application of muscle anti-aging

• Project/Area Number: 21580412
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Nagoya University
• Principal Investigator: NADANO Daita 名古屋大学, 生命農学研究科, 准教授 (00228074)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 細胞増殖 / 分化 / 発現制御 / 翻訳 / 細胞・組織 / シグナル伝達 / 発生・分化 / 老化

Research Abstract

The human protein, named bystin, has been indicated to benecessary for self-renewal of undifferentiated cells such as stem cells and to be important for translational activation in cells, and thus has a unique property playing two distinct roles. Because the molecular mechanisms for these roles were unclear, proteinscooperating with bystin were surveyed, and novel factors were identified and analyzed. These findings are expected to lead to useful mechanisms applicable to differentiation of muscular cells, which requires cell number increase and growth.

Research Products

• [Journal Article] The murine Gcap14gene encodes a novel microtubule binding and bundling protein. (2012)
  • Author(s): Hosono, H., Yamaguchi, N., Oshima, K., Matsuda, T., and Nadano, D.
  • Journal Title: FEBS Lett Volume: 586(10) Issue: 10 Pages: 1426-1430
  • DOI: 10.1016/j.febslet.2012.04.008
  • Peer Reviewed
• [Journal Article] Secretion of three enzymes for fatty acid synthesis into mouse milk in association with fat globules, and rapid decrease of the secreted enzymes by treatment with rapamycin. (2011)
  • Author(s): Moriya, H., Uchida, K., Okajima, T., Matsuda, T., and Nadano, D.
  • Journal Title: Arch. Biochem. Biophys Volume: 508(1) Issue: 1 Pages: 87-92
  • DOI: 10.1016/j.abb.2011.01.015
  • NAID: 120004384226
  • Peer Reviewed
• [Journal Article] Secretion of three enzymes for fatty acid synthesis into mouse milk in association with fat globules, and rapid decrease of the secreted enzymes by treatment with rapamycin (2011)
  • Author(s): Moriya, H.(第一著者)
  • Journal Title: Arch.Biochem.Biophys. Volume: 508 Pages: 87-92
  • Peer Reviewed
• [Journal Article] Identification of heterogeneous nuclear ribonucleoprotein A/B as a cytoplasmic mRNA-binding protein in early involution of the mouse mammary gland (2010)
  • Author(s): Taga, Y., Miyoshi, M., Okajima, T., Matsuda, T., and Nadano, D.
  • Journal Title: Cell Biochem. Function Volume: 28(4) Issue: 4 Pages: 321-328
  • DOI: 10.1002/cbf.1662
  • Peer Reviewed
• [Journal Article] Proteomic analysis of rodent ribosomes revealed heterogeneity including ribosomal proteins L10-like, L22-like 1, andL39-like (2010)
  • Author(s): Sugihara, Y., Honda, H., Iida, T., Morinaga, T., Hino, S., Okajima, T., Matsuda, T., and Nadano, D.
  • Journal Title: J. Proteome Res Volume: 9(3) Issue: 3 Pages: 1351-1366
  • DOI: 10.1021/pr9008964
  • Peer Reviewed
• [Journal Article] Identification of heterogeneous nuclear ribonucleoprotein A/B as a cytoplasmic mRNA-binding protein in early involution of the mouse mammary gland. (2010)
  • Author(s): Taga, Y(第一著者)
  • Journal Title: Cell Biochem. Function Volume: 280 Pages: 321-328
  • Peer Reviewed
• [Journal Article] Proteomic analysis of rodent ribosomes revealed heterogeneity including ribosomal proteins L10-like, L22-like 1, and L39-like. (2010)
  • Author(s): Sugihara, Y.(第一著者)
  • Journal Title: J. Proteome Res. VOL. 9 Pages: 1351-1366
  • Peer Reviewed
• [Presentation] 発達段階に依存した精巣特異的リボソームタンパク質L10-likeの発現 (2012)
  • Author(s): 佐土原英司・杉原圭彦・大島健司・松田幹・灘野大太
  • Organizer: 2012年度日本農芸化学会全国大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-24
• [Presentation] 発達段階に依存した精巣特異的リボソームタンパク質L10-likeの発現 (2012)
  • Author(s): 佐土原英司(代表者)
  • Organizer: 2012年度日本農芸化学会全国大会
  • Place of Presentation: 京都女子大学(京都府)
  • Year and Date: 2012-03-24
• [Presentation] 精巣特異的リボソームタンパク質の発達段階に依存した発現 (2012)
  • Author(s): 佐土原英司・杉原圭彦・大島健司・松田幹・灘野大太
  • Organizer: 第1回リボソームミーティング
  • Place of Presentation: 広島大学
  • Year and Date: 2012-03-15
• [Presentation] 精巣特異的リボソームタンパク質の発達段階に依存した発現 (2012)
  • Author(s): 灘野大太(代表者)
  • Organizer: 第1回リボソームミーティング
  • Place of Presentation: 広島大学(広島県)
  • Year and Date: 2012-03-15
• [Presentation] プロテオミクスによる哺乳類リボソームにおける不均一性の解析 (2010)
  • Author(s): 灘野大太・杉原圭彦・本多弘樹・日野真吾・岡島徹也・松田幹
  • Organizer: 2010年度日本農芸化学会全国大会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-03-30
• [Presentation] プロテオミクスによる哺乳類リボソームにおける不均一性の解析 (2010)
  • Author(s): 灘野大太(発表代表名)
  • Organizer: 2010年度日本農芸化学会全国大会シンポジウム「リボソーム生合成研究の新展開」
  • Place of Presentation: 東京大学駒場キャンパス(東京都)
  • Year and Date: 2010-03-30
• [Presentation] Proteomic analysis of rodent ribosomes revealedheterogeneity including ribosomalproteins (2009)
  • Author(s): Sugihara, Y., Honda, H., Okajima, T., Matsuda, T., and Nadano, D.
  • Organizer: The 8th international conference onribosome biogenesis
  • Place of Presentation: Regensburg, Germany
  • Year and Date: 2009-08-28