Analyses of Translocation Intermediates Formed during Protein Import into Chloroplasts in Higher Plants

• Project/Area Number: 21580415
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Ehime University
• Principal Investigator: AKITA Mitsuru 愛媛大学, 農学部, 准教授 (50335890)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 蛋白質膜透過 / 葉緑体 / 前駆体蛋白質 / 蛋白質間相互作用 / 蛋白質膜秀過

Research Abstract

Nuclear-encoded chloroplastic proteins are imported into chloroplasts by utilizing the unique translocon embedded in the double-envelope membranes from the cytosol after translated as precursors. Under limited energy conditions, precursors are trapped at the translocon to form the early-translocation intermediates. During the research period, I have established the experimental systems to analyze the protein-protein interactions between precursors and the translocon component(s) and intra-interactions within the translocon.

Research Products

• [Journal Article] ppGpp inhibits peptide elongation cycle of chloroplast translation system in vitro (2012)
  • Author(s): Nomura, Y., Takabayashi, T., Kuroda, H., Yukawa, Y., Sattasuk, K., Akita, M., Nozawa, A., and Tozawa, Y.
  • Journal Title: Plant Mol. Biol. Volume: 78(1-2) Pages: 185-196
  • Peer Reviewed
• [Journal Article] In vitro protein import of a putative amino acid transporter from Arabidopsis thaliana into chloroplasts and its suborganellar localization (2011)
  • Author(s): Sattasuk, K., Nozawa, A., Tozawa, Y., Kakinuma, Y., and Akita, M.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 75(11) Pages: 2200-2206
  • NAID: 10030396915
  • Peer Reviewed
• [Journal Article] In vitro fluorescent analysis of preprotein import into chloroplasts (2011)
  • Author(s): Sattasuk, K., Inoue, H., and Akita, M.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 75(10) Pages: 2001-2007
  • NAID: 10029873114
  • Peer Reviewed
• [Journal Article] In Vitro Fluorescent Analysis of Preprotein Import into Chloroplasts (2011)
  • Author(s): Sattasuk, K., Inoue, H., Akita, M.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 10 Pages: 2001-2007
  • DOI: 10.1271/bbb.110438
  • NAID: 10029873114
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] In Vitro Protein Import of a Putative Amino Acid Transporter from Arabidopsis thaliana into Chloroplasts and Its Suborganellar Localization (2011)
  • Author(s): Sattasuk, K., Nozawa, A., Tozawa, Y., Kakinuma Y., Akita, M.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 11 Pages: 2200-2206
  • DOI: 10.1271/bbb.110489
  • NAID: 10030396915
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Evaluating the Energy-Dependent "Binding" in the Early Stage of Protein Import into Chloroplasts (2009)
  • Author(s): Mitsuru Akita, Hitoshi Inoue
  • Journal Title: Methods in Enzymology 466 Pages: 44-65
• [Presentation] シロイヌナズナ由来大腸菌TyrP相同蛋白質の機能解析 (2012)
  • Author(s): Sattasuk, K., 清水綾子, 秋田充
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-25
• [Presentation] In vitro protein import of a putative amino acid transporter from Arabidopsis thaliana into chloroplasts and its suborganellar localization (2011)
  • Author(s): Sattasuk, K., Nozawa, A., Tozawa, Y., Kakinuma, Y., and Akita, M.
  • Organizer: Protein Island Matsuyama International Symposium
  • Place of Presentation: Matsuyama
  • Year and Date: 2011-09-22
• [Presentation] In Vitro Protein Import of a Putative Amino Acid Transporter from Arabidopsis thaliana into Chloroplasts and Its Suborganellar Localization (2011)
  • Author(s): Sattasuk, K., Nozawa, A., Tozawa, Y., Kakinuma Y., Akita, M.
  • Organizer: Protein Island Matsuyama (PIM) International Symposium 2011
  • Place of Presentation: 松山市
  • Year and Date: 2011-09-22
• [Presentation] The targeting properties of the putative chloroplastic protein in the inner envelope membrane (2010)
  • Author(s): Sattasuk, K. and Akita, M.
  • Organizer: The 3rd International Symposium on" Protein Community"
  • Place of Presentation: Nara
  • Year and Date: 2010-09-14
• [Presentation] The targeting properties of the putative chloroplastic protein in the inner envelope membrane (2010)
  • Author(s): Sattasuk Kwanchanok, Mitsuru Akita
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: 奈良
• [Presentation] The analysis of the movement of the targeting signal on the precursor protein during the transition of early translocation intermediates formed during protein import into chloroplasts (2009)
  • Author(s): Akita, M. and Inoue, H.
  • Organizer: Plant Biology 2009, Joint Annual Meetings of the American Society of Plant Biologists and the Phycological Society of America
  • Place of Presentation: Honolulu, HI, USA
• [Presentation] Targeting of a chloroplastic inner envelope membrane protein carrying a putative targeting signal (2009)
  • Author(s): Sattasuk, K. and Akita, M.
  • Organizer: Plant Biology 2009, Joint Annual Meetings of the American Society of Plant Biologists and the Phycological Society of America
  • Place of Presentation: Honolulu, HI, USA
• [Presentation] The analysis of the movement of the targeting signal on the precursor protein during the transition of early translocation intermediates formed during protein import into chloroplasts (2009)
  • Author(s): Mitsuru Akita, Hitoshi Inoue
  • Organizer: Plant Biology 2009(Joint Annual Meetings of the American Society of Plant Biologists and the Phycological Society of America)
  • Place of Presentation: 米国ハワイ州ホノルル市
• [Presentation] Targeting of a chloroplastic inner envelope membrane protein carrying a putative targeting signal (2009)
  • Author(s): Sattasuk Kwanchanok, Mitsuru Akita
  • Organizer: Plant Biology 2009(Joint Annual Meetings of the American Society of Plant Biologists and the Phycological Society of America)
  • Place of Presentation: 米国ハワイ州ホノルル市
• [Book] Evaluating the energy-dependent" binding" in the early stage of protein import into chloroplasts (2009)
  • Author(s): Akita, M. and Inoue, H.
  • Publisher: Burlington : Academic Press(In Michael L. Johnson, M. L., Holt, J. M., and Ackers, G. K.(eds))