Formation of microdomains in cell membranes investigated by microscopic analyses of structures of membranes and proteins

• Project/Area Number: 21590037
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: MIURA Takashi 東北大学, 大学院・薬学研究科, 准教授 (30222318)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 構造生物学 / 脂質膜 / 脂質ラフト / 細胞膜 / 神経変性疾患 / ラマン分光法 / 膜ドメイン

Research Abstract

The monomeric amyloid・peptide (A ・・ binds to the phosphatidylcholine membrane in the lamellar gel phase but not in the liquid crystalline phase. Tightly packed phosphatidylcholine membranes appear to serve as a platform for non-electrostatic binding and self-association of A・. On the other hand, aggregated A・peptides bind to the phosphatidylcholine membrane both in the lamellar gel and the liquid crystalline phases. The binding of aggregated A・significantly reduces fluidity of the membrane in the liquid crystalline phase.

Research Products

• [Journal Article] Conformational Regulation of Amyloid Beta-Peptide by Lipid Membranes and Metal Ions. (2010)
  • Author(s): Takashi Miura, Mayumi Yoda, Chihiro Tsutsumi, Kiyoko Murayama and Hideo Takeuchi
  • Journal Title: Yakugaku Zasshi Volume: 130 Pages: 495-501
  • Peer Reviewed
• [Journal Article] Regulation of secondary structure of peptides by lipid membranes. (2010)
  • Author(s): Takashi Miura
  • Journal Title: Seibutsu Butsuri Volume: 50 Pages: 184-185
  • NAID: 10026569452
  • Peer Reviewed
• [Journal Article] Role of His16 in the structural flexibility of the C-terminal region of human endothelin-1 (2010)
  • Author(s): Hirotsugu Hiramatsu, Hiroki Aduma, Yuriko Tanaka, Takashi Miura, and Hideo Takeuchi
  • Journal Title: J. Mol. Struct Volume: 976 Pages: 328-332
  • Peer Reviewed
• [Journal Article] Role of His16 in the structural flexibility of the C-terminal region of human endothelin-1 (2010)
  • Author(s): Hirotsugu Hiramatsu, Hiroki Aduma, Yuriko Tanaka, Takashi Miura, Hideo Takeuchi
  • Journal Title: J.Mol.Struct. Volume: 976 Pages: 328-332
  • Peer Reviewed
• [Journal Article] 脂質膜と金属イオンによるアミロイドβペプチドのコンホメーション制御 (2010)
  • Author(s): 三浦隆史, 依田真由美, 堤千裕, 村山紀代子, 竹内英夫
  • Journal Title: 薬学雑誌 Volume: 130 Pages: 495-501
  • NAID: 130000259537
  • Peer Reviewed
• [Journal Article] 脂質膜によるペプチドの2次構造制御 (2010)
  • Author(s): 三浦隆史
  • Journal Title: 生物物理 Volume: 50 Pages: 184-185
  • NAID: 10026569452
  • Peer Reviewed
• [Journal Article] Interactions between histidine and tryptophan residues in the BM2 proton channel from influenza B virus. (2009)
  • Author(s): K. Otomo, A. Toyama, T. Miura, H. Takeuchi
  • Journal Title: J. Biochem Volume: 145 Pages: 543-54
  • NAID: 10026302342
  • Peer Reviewed
• [Journal Article] Interactions Between Histidine and Tryptophan Residues in the BM2 Prot on Channel from Influenza B Virus (2009)
  • Author(s): Kohei Otomo, Akira Toyama, Takashi Miura, Hideo Takeuchi
  • Journal Title: J.Biochem. 145 Pages: 543-554
  • Peer Reviewed
• [Presentation] スフィンゴ脂質を含む脂質膜とアミロイドβペプチドとの相互作用 (2011)
  • Author(s): 東海林秀樹、三浦隆史、竹内英夫
  • Organizer: 第50回日本薬学会東北支部大会
  • Place of Presentation: 仙台
  • Year and Date: 2011-10-30
• [Presentation] HIV-1ウイルスのVprタンパク質におけるカチオン-π相互作用 (2011)
  • Author(s): 上山貴之、三浦隆史、竹内英夫
  • Organizer: 第50回日本薬学会東北支部大会
  • Place of Presentation: 仙台
  • Year and Date: 2011-10-30
• [Presentation] Ctr1による銅イオン細胞内取り込みの分子メカニズムMolecular mechanism of copper uptake by Ctr1 (2011)
  • Author(s): 三浦隆史
  • Organizer: 化学系学協会東北大会
  • Place of Presentation: 仙台
  • Year and Date: 2011-09-17
• [Presentation] Ctr1による銅イオン細胞内取り込みの分子メカニズム (2011)
  • Author(s): 三浦隆史
  • Organizer: 化学系学協会東北大会
  • Place of Presentation: 仙台(招待講演)
  • Year and Date: 2011-09-17
• [Presentation] Structural Polymorphism and Copper Transport Function of the Extracellular N-Terminal Domain of Ctrl (2010)
  • Author(s): Takashi Miura, Shiho Saito, Ako Okawara, Kenji Itoh, Shintaro Yamazaki, Hideo Takeuchi
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: 米国、ホノルル
  • Year and Date: 2010-12-18
• [Presentation] Structural Polymorphism and Copper Transport Function of the Extracellular N-Terminal Domain of Ctr1 (2010)
  • Author(s): Takashi Miura, Shiho Saito, Ako Okawara, Kenji Itoh, Shintaro Yamazaki and Hideo Takeuchi
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2010)
  • Place of Presentation: Honolulu, U. S. A
• [Presentation] 脂質分子のパッキングがアミロイドβペプチドと脂質膜の相互作用に与える影響 (2010)
  • Author(s): 東海林秀樹、依田真由美、三浦隆史、竹内英夫
  • Organizer: 第48回生物物理学会年会
  • Place of Presentation: 仙台
• [Presentation] タンパク質中におけるカチオン-π相互作用の強さの評価 (2010)
  • Author(s): 上山貴之、三浦隆史、竹内英夫
  • Organizer: 第48回生物物理学会年会
  • Place of Presentation: 仙台
• [Presentation] エンドセリン1の構造安定性に対するHis16の役割 (2010)
  • Author(s): 平松弘嗣、我妻弘基、田中友里子、三浦隆史、竹内英夫
  • Organizer: 第37回生体分子科学討論会
  • Place of Presentation: 山口
• [Presentation] 脂質膜により誘起されるタウ微小管結合領域の構造転移と線維形成 (2009)
  • Author(s): 益山純英, 飛田英里, 三浦隆史, 竹内英夫
  • Organizer: 第48回日本薬学会東北支部大会
  • Place of Presentation: 仙台
  • Year and Date: 2009-10-18
• [Presentation] 脂質膜によるタウ微小管結合領域の構造転移と線維形成 (2009)
  • Author(s): 益山純英, 飛田英里, 三浦隆史, 竹内英夫
  • Organizer: 第36回生体分子科学討論会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2009-06-20
• [Presentation] 脂質膜によるタウ微小管結合領域の構造転移と線維形成 (2009)
  • Author(s): 益山純英, 飛田英里, 三浦隆史, 竹内英夫
  • Organizer: 第36回生体分子科学討論会
  • Place of Presentation: 札幌
• [Remarks] 東北大学大学院薬学研究科、生物構造化学分野ホームページ
  • URL: http://www.pharm.tohoku.ac.jp/~kouzou/kouzou.html
• [Remarks]
  • URL: http://www.pharm.tohoku.ac.jp/~kouzou/kouzou.html