Development of the prominent labeling reagents for LC/MS/MS and sensitive analysis of the biomolecules

• Project/Area Number: 21590039
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: SANTA Tomofumi 東京大学, 大学院・薬学系研究科, 准教授 (30187306)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 分析化学 / 標識試薬 / クロマトグラフィー / 質量分析 / LC/MS/MS / 生体分子 / 新生児マススクリーニング

Research Abstract

Liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) is widely used method. In this research, several labeling reagents were synthesized to enhance the sensitivity and selectivity in LC/MS/MS. These reagents enabled sensitive analysis of the biomolecules such as diseases markers. These methods were useful for the screening of the inherited metabolic disorders.

Research Products

• [Journal Article] Derivatization reagents in liquid chromatography/electrospray ionization tandem mass spectrometry. (2011)
  • Author(s): Santa T.
  • Journal Title: Biomedical Chromatography Volume: 25 Issue: 1-2 Pages: 1-10
  • DOI: 10.1002/bmc.1548
  • Peer Reviewed
• [Journal Article] Compounds having thiourea moiety as derivatization reagents in liquid chromatography/ electrospray ionization-tandem mass spectrometry(LC/ESI-MS/MS) (2011)
  • Author(s): Inoda H, Nishiyama T, Yoshikado T, Suwanai Y, Santa T.
  • Journal Title: Synthesis of derivatization reagents for carboxylic acids. Biomedical Chromatography Volume: 25 Issue: 6 Pages: 635-640
  • DOI: 10.1002/bmc.1501
  • Peer Reviewed
• [Journal Article] Diagnosis of glutaric aciduria type 1 by measuring3-hydroxyglutaric acid in dried urine spots by liquid chromatography tandem mass spectrometry. (2011)
  • Author(s): Al-Dirbashi OY, Kolker S, Ng D, Fisher L, Rupar T, Lepage N, Rashed MS, Santa T, Goodman SI, Geraghty MT, Zschocke J, Christensen E, Hoffmann GF, Chakraborty P.
  • Journal Title: Journal of Inherited Metabolic Disorders Volume: 34 Issue: 1 Pages: 173-180
  • DOI: 10.1007/s10545-010-9223-2
  • Peer Reviewed
• [Journal Article] Isothiocyanates as derivatization reagents for amines in liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS). (2010)
  • Author(s): Santa T.
  • Journal Title: Biomedical Chromatography Volume: 24 Issue: 9 Pages: 915-918
  • DOI: 10.1002/bmc.1352
  • Peer Reviewed
• [Journal Article] Isothiocyanates as derivatization reagents for amines in liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) (2010)
  • Author(s): Santa T
  • Journal Title: Biomedical Chromatography Volume: 24 Pages: 915-918
  • Peer Reviewed
• [Journal Article] Synthesis of benzofurazan derivatization reagents for short chain carboxylic acids inliquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS). (2009)
  • Author(s): Santa T, Al-Dirbashi OY, Yoshikado T, Fukushima T, Imai K.
  • Journal Title: Biomedical Chromatography Volume: 23 Issue: 4 Pages: 443-446
  • DOI: 10.1002/bmc.1124
  • Peer Reviewed
• [Journal Article] Zellweger Syndrome Caused by PEX13 Deficiency: Report of Two Novel Mutations. (2009)
  • Author(s): Al-Dirbashi OY, Shaheen R, Al-Sayed M, Al-Dosari M, Makhseed N, Abu Safieh L, Santa T, Meyer BF, Shimozawa N, Alkuraya FS.
  • Journal Title: American Journal of Medical Genetics Part A Volume: 149A Issue: 6 Pages: 1219-1223
  • DOI: 10.1002/ajmg.a.32874
  • Peer Reviewed
• [Journal Article] Synthesis of benzofurazan derivatization reagents for short chain carboxylic acids in liquid chromatography/electrospray ionization-tandem mass spectrometry. (2009)
  • Author(s): Santa, Tomofumi
  • Journal Title: Biomedical Chromatography Volume: 23 Pages: 443-446
  • Peer Reviewed
• [Journal Article] Zellweger syndrome caused by PEX13 deficiency : report of two novel mutations. (2009)
  • Author(s): Al-Dirbashi, Osama Y
  • Journal Title: American Journal of Medical Genetics part A Volume: 149A Pages: 1219-1223
  • Peer Reviewed
• [Presentation] LC/ESI-MS/MS用標識試薬の開発:-NHCSNH-構造を有する標識試薬. (2011)
  • Author(s): 三田智文
  • Organizer: 第24回バイオメディカル分析科学シンポジウム
  • Place of Presentation: 大山ロイヤルホテル(鳥取県)
  • Year and Date: 2011-09-01
• [Presentation] LC/ESI-MS/MS 用標識 試薬の開 発: thiourea 構造を有する標識試薬. (2011)
  • Author(s): 西山泰平、猪田浩隆、吉門崇、三田智文.
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
• [Presentation] Analysis of3-hydroxyglutalic acid in dried urine spots by LC-MS/MS; application for the diagnosis of glutaric aciduria type 1. (2011)
  • Author(s): Al-Dirbashi OY, Kolker S, Ng D, Fisher L, Rupar T, Lepage N, Rashed MS, Santa T, Goodman SI, Geraghty MT, Zschocke J, Christensen E, Hoffmann GE, Chakraborty P.
  • Organizer: SSIEM (Society for the Study of Inborn Error of Metabolism) annual symposium, Geneva,
  • Place of Presentation: Switzerland
• [Presentation] Novel approaching for the determination of methycitrate in dried blood spots (DBS) by liquid chromatography tandem mass spectrometry. (2011)
  • Author(s): Al-Dirbashi OY, McIntosh N, McRoberts C, Rashed MS, Geraghty MT, Fisher L, Santa T, Chakraborty P.
  • Organizer: SSIEM (Society for the Study of Inborn Error of Metabolism) annual symposium, Geneva
  • Place of Presentation: Switzerland
• [Presentation] LC/ESI-MS/MS 用標識試薬の開発:-NHCSNH- 構造を有する標識試薬 (2011)
  • Author(s): 三田智文
  • Organizer: 第24回バイオメディカル分析科学シンポジウム
  • Place of Presentation: 大山
• [Presentation] Diagnosis of low excretor GA1 by measuring 3-hydroxyglutaric acid in dried urine spots (2010)
  • Author(s): Al-Dirbashi, Osama Y
  • Organizer: The 2010 Newborn Screening and Genetic Testing Symposium
  • Place of Presentation: Orlando, USA
  • Year and Date: 2010-05-03
• [Presentation] Novel approach for quantification of fatty acid markers of peroxisomal disorders by LC-MS/MS. (2009)
  • Author(s): Al-Dirbashi, Osama Y
  • Organizer: The annual meeting of the Garrod Association
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2009-05-01
• [Remarks]
  • URL: http://www.f.u-tokyo.ac.jp/~kaiseki/
• [Remarks]
  • URL: http://www.f.u-tokyo.ac.jp/~kaiseki/