Motional linkage between voltage sensor and ion gate in voltage-dependent ion channels
Project/Area Number |
21590040
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Physical pharmacy
|
Research Institution | The University of Tokyo |
Principal Investigator |
OSAWA Masanori 東京大学, 大学院・薬学系研究科, 助教 (60361606)
|
Co-Investigator(Kenkyū-buntansha) |
SHIMADA Ichio 東京大学, 大学院・薬学系研究科, 教授 (70196476)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 構造生物学 / 電位依存性イオンチャネル / 電位センサードメイン / gating modifier toxin / 分子認識様式 / イオンチャネル / NMR / イオンゲート |
Research Abstract |
Voltage-dependent ion channels consist of a voltage-sensor domain(VSD) and a pore domain possessing the ion permeation pathway. This study demonstrated how VSD changes its conformation in a voltage-dependent manner, by a disulfide-locking method using a series of double cysteine mutants. In addition, the binding mode between VSD and its inhibiting toxin, analyzed by NMR, revealed the inhibition mechanism of the toxin.
|
Report
(4 results)
Research Products
(43 results)