| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
We established Live Single-cell Mass Spectrometry which enables to clarify exhaustive molecular content of single cell recovered by a metal-coated glass tip following direct mass spectrometric analysis. In this study, we aimed to increase sensitivity and kinds of molecules by modifying the inner surface of the glass tips. We made the tips with hydrophilic or hydrophobic treatment which allow selective trapping of polar compounds or lipophilic compounds respectively. We proved treated tips to trap standard compound as well as molecules contained in single cell. Thus, detection of molecules with specific physical property can be detected by Live Single-cell Mass Spectrometry using this tip.
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