Development and application of a chemiluminescent measurement method folic acid related compounds as an arteriosclerosis marker
| Project/Area Number |
21590043
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
WADA Mitsuhiro 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (40295093)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 分析化学 / 臨床分析化学 / ルテニウム錯体 / 化学発光検出 / 葉酸関連化合物 / ホモシステイン / 化学発光 / 葉酸 |
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| Research Abstract |
A ruthenium(II)-bipyridine complex chemiluminescent measurement of folic acid related compounds has been examined and developed a useful HPLC-CL method. The developed method was successfully applied to quantify folic acid in tablets and supplements. This means the method might be useful for the quality control of these materials. On the other hand, application of the method to folic acid related compounds except for 5-methyltetrahydrofolic acid in biomaterials was not always successful due to the less stability of these compounds and the lower sensitivity of the method. Improvement of the method concerning the conditions such as stabilizing agent and so on, are required.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] The association between atherosclerosis and plasma homocycteine concentration in the general population residing on remote islands in Japan2011
Author(s)
M. Nakazato, N. Takamura, K. Kadota, H. Yamasaki, H. Mukae, Y. Kusano, K. Nakashima, Y. Ozono, K. Aoyagi, S. Kohno, T. Maeda
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Journal Title
Acta Med. Nagasaki
Volume: 55
Pages: 47-54
Related Report
Peer Reviewed
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[Journal Article] The association between atherosclerosis and plasma homocycteine concentration in the general population residing on remote islands in Japan2011
Author(s)
M.Nakazato, N.Takamura, K.Kadota, H.Yamasaki, H.Mukae, Y.Kusano, K.Nakashima, Y.Ozono, K.Aoyagi, S.Kohno, T.Maeda
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Journal Title
Acta Med.Nagasaki
Volume: 55
Pages: 47-54
Related Report
Peer Reviewed
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[Journal Article] A validated HPLC-fluorescence method with a semi-micro column for routine determination of homocysteine cysteine and cysteamine, and the relation between the thiol derivatives in normal human plasma2009
Author(s)
S. Ichinose, M. Nakamura, M. Maeda, R. Ikeda, M. Wada, M. Nakazato, Y. Ohba, N. Takamura, T. Maeda, R. Ikeda, K. Aoyagi, K. Nakashima
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Journal Title
Biomed. Chromatogr.
Volume: 23
Pages: 935-939
Related Report
Peer Reviewed
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[Journal Article] A validated HPLC-fluorescence method with a semi-micro column for routine determination of homocysteine, cysteine and cysteamine, and the relation between the thiol derivatives in normal human plasma2009
Author(s)
S.Ichinose, M.Nakamura, M.Maeda, R.Ikeda, M.Wada, M.Nakazato, Y.Ohba, N.Takamura, T.Maeda, K.Aoyagi, K.Nakashima
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Journal Title
Biomedical Chromatography 23巻
Pages: 935-939
Related Report
Peer Reviewed
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[Presentation] 健常人血漿チオール化合物の定量とその解析2009
Author(s)
前田麻由, 中島真裕美, 池田理恵, 和田光弘, 中里未央, 前田隆浩, 高村昇, 青柳潔, 中島憲一郎
Organizer
第49回日本臨床化学会年次学術集会
Place of Presentation
長崎大学医学部
Year and Date
2009-09-19
Related Report
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