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Analysis of Modification with polyamines and investigation of the physiological significance

Research Project

Project/Area Number 21590044
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionJosai University

Principal Investigator

SHIRAHATA Akira  城西大学, 薬学部, 教授 (50150107)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsポリアミン / 分子修飾 / 腸管吸収 / アシル基転移反応 / アシルスペルミン / N-acyltransferase (NAT) / スペルミン / アシルアミン / N-acyltransferase(NAT) / ポイアミンアナログ / ポリアミンアナログ
Research Abstract

In this study, polyamines that remain to be resolved for detailed roles, was focused to investigate novel cellular functions. The results illustrated that the transfer of polyamines to acyl group occurred during the acyl transfer reaction between cellular phospholipids. Additionally, polyamine-conjugated compounds were found to affect intestinal absorption of water-soluble big molecules.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (53 results)

All 2012 2011 2010 2009 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (29 results) Remarks (7 results)

  • [Journal Article] Polyamine metabolism is involved in adipogenesis of 3T3-L1 cells2012

    • Author(s)
      Ikumi Ishii, Yoshihiko Ikeguchi, Hiroshi Mano, Masahiro Wada, Anthoby E. Pegg, Akira Shirahata
    • Journal Title

      Amino Acids

      Volume: 42 Issue: 2-3 Pages: 619-626

    • DOI

      10.1007/s00726-011-1037-5

    • NAID

      120005520696

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A potential estrogen mimetic effect of a bis(ethyl)polyamine analogue on estrogen receptor positive MCF-7 breast cancer cells2012

    • Author(s)
      Nayvelt I, John S, Hsu HC, Yang P, Liu W, Das G, Hyvonen MT, Alhonen L, Keinanen TA, Shirahata A, Patel R, Thomas T, Thomas TJ
    • Journal Title

      Amino Acids

      Volume: (in press) Issue: 2-3 Pages: 899-911

    • DOI

      10.1007/s00726-011-1005-0

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of Gastrointestinal Absorption of Ovalbumin Caused by Spermine Induces an Increase in Plasma Histamine Levels in Mice Sensitized to Ovalbumin2011

    • Author(s)
      Yoshiaki Sugita, Koichi Takao, Yoshihiko Sugino, Ryoko Kuwabara, Akira Shirahara
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 34 Issue: 3 Pages: 415-419

    • DOI

      10.1248/bpb.34.415

    • NAID

      130000657822

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescent substrates for polyamine catabolic enzymes and transport2011

    • Author(s)
      Koichi Takao, Akira Shirahara
    • Journal Title

      Methods Mol. Biol.

      Volume: 720 Pages: 463-474

    • DOI

      10.1007/978-1-61779-034-8_30

    • ISBN
      9781617790331, 9781617790348
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of gastrointestinal absorption of ovalbumin caused by spermine induces an increase in plasma histamine levels in mice sensitized to ovalbumin.2011

    • Author(s)
      Sugita, Y.
    • Journal Title

      Biol.Pharm.Bull.

      Volume: 34 Pages: 425-429

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the primary structure and post-translational modification of rat S-adenosylmethionine decarboxylase.2011

    • Author(s)
      Takao, K.
    • Journal Title

      Methods Mol.Biol.

      Volume: 720 Pages: 463-474

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conformational Stabilization of Rat S-Adenosylmethionine Decarboxylase by Putrescine2010

    • Author(s)
      Makiko Wada, Akira Shirahata
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 33 Issue: 11 Pages: 1800-1805

    • DOI

      10.1248/bpb.33.1800

    • NAID

      130000402299

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Assay of N1-Acetylpolyamine Oxidase Activity with N1,N11-Didansylnorspermine as the Substrate by Ion-Pair Reversed Phase High Performance Liquid Chromatography2010

    • Author(s)
      Koichi Takao, Yoshiaki Sugita, Akira Shirahara
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 33 Issue: 7 Pages: 1089-1094

    • DOI

      10.1248/bpb.33.1089

    • NAID

      130000300268

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the Primary Structure and Post-translational Modification of Rat S-Adenosylmethionine Decarboxylase2010

    • Author(s)
      Makiko Wada, Akira Shirahata
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 33 Issue: 5 Pages: 891-894

    • DOI

      10.1248/bpb.33.891

    • NAID

      130000248054

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation method for polyamine uptake by N(1)-dansylspermine2010

    • Author(s)
      Koichi Takao, Yoshiaki Sugita, Akira Shirahata
    • Journal Title

      Amino Acid

      Volume: 38 Issue: 2 Pages: 533-539

    • DOI

      10.1007/s00726-009-0414-9

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Conformational stabilization of rat s-adenosylmethionine decarboxylase by putrescine.2010

    • Author(s)
      Wada, M.
    • Journal Title

      Biol.Pharm.Bull.

      Volume: 33 Pages: 1800-1805

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assay of N1-acetylpolyamine oxidase activity with N^1, N^<11>-didansylnorspermine as the substrate by ion-pair reversed phase high performance liquid chromatography.2010

    • Author(s)
      Takao, K.
    • Journal Title

      Biol.Pharm.Bull.

      Volume: 33 Pages: 1089-1094

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the primary structure and post-translational modification of rat S-adenosylmethionine decarboxylase.2010

    • Author(s)
      Wada, M.
    • Journal Title

      Biol.Pharm.Bull.

      Volume: 33 Pages: 891-894

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation method for polyamine uptake by N(1)-dansylsperm-ine, Amino Acid2010

    • Author(s)
      Takao, K.
    • Journal Title

      Amino Acid 38

      Pages: 533-539

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the primary structure and post-translational modification of rat S-adenosylmethionine decarboxylase2010

    • Author(s)
      Wada, M.
    • Journal Title

      Biol.Pharm.Bull. (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A conceptual model of the polyamine binding site of N1-acetylpolyamine oxidase developed from a study of polyamine derivatives2009

    • Author(s)
      Koichi Takao, Satoko Shibata, Tomohiro Ozawa, Makiko Wada, Yoshiaki Sugita, Keijiro Samejima, and Akira Shirahata
    • Journal Title

      Amino Acids

      Volume: 37 Issue: 2 Pages: 401-405

    • DOI

      10.1007/s00726-008-0168-9

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A conceptual model of the polyamine binding site of N^1-acetyl polyamine oxidase developed from a study of polyamine derivatives2009

    • Author(s)
      Takao, K.
    • Journal Title

      Amino Acid 37

      Pages: 401-405

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] エテノ蛍光誘導体化法を用いるS-アデノシルメチオニン脱炭酸酵素活性測定法の開発2011

    • Author(s)
      山嵜健一
    • Organizer
      日本ポリアミン学会 第2回年会
    • Place of Presentation
      栃木
    • Year and Date
      2011-01-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Histochemistry study on abbrant metabolism in bone and chondrocyte of a spermine-deficient Gy mouse2011

    • Author(s)
      Ayasa Tajima, Yoshihiko Ikeguchi, Mami Saita, Sachie Nakatani, Eri Arikawa-Hirasawa, Muneaki Ishijima, Anthony E. Pegg, Masahiro Wada, Hiroshi Mano and Akira Shirahata
    • Organizer
      2011 Gordon Research Conference on Polyamines
    • Place of Presentation
      米国
    • Related Report
      2011 Final Research Report
  • [Presentation] Electrophoresis analysis for the enhancement in DNA strand-exchange activity of Rad51 by polyamines2011

    • Author(s)
      Yoshihiko Ikeguchi, Masaki Sugawara, Motoki Takaku, Kenichi Yamazaki, Anthony E. Pegg, Hitoshi Kurumizaka, Akira Shirahata
    • Organizer
      2011 Gordon Research Conference on Polyamines
    • Place of Presentation
      米国
    • Related Report
      2011 Final Research Report
  • [Presentation] エテノ蛍光誘導体化法を用いるS-アデノシルメチオニン脱炭酸化酵素活性測定法の生体試料への応用2011

    • Author(s)
      山嵜健一、池口文彦、丹波卓也、白幡晶
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Related Report
      2011 Final Research Report
  • [Presentation] 電気泳動によるスペルミンの遺伝子相同組換えに及ぼす影響の解析2011

    • Author(s)
      菅原将希、池口文彦、神沼路子、山嵜健一、高久誉大、胡桃坂仁1、白幡晶
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Related Report
      2011 Final Research Report
  • [Presentation] スペルミン合成酵素遺伝子改変マウスにおける骨代謝・軟骨代謝異常の解析2011

    • Author(s)
      田島彩沙、池口文彦、齋田麻実、中谷祥恵、平澤恵理、石島旨章、和田政裕、真野博、白幡晶
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス及びHepG2細胞を用いる肝臓における脂質蓄積に及ぼすポリアミンの影響2011

    • Author(s)
      片倉美佳、下川宗也、岩城孝宏、石井育美、山嵜健一、池口文彦、白幡晶
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Related Report
      2011 Final Research Report
  • [Presentation] エテノ蛍光誘導体化法を用いるS-アデノシルメチオニン脱炭酸酵素活性測定法の開発2011

    • Author(s)
      山嵜健一、池口文彦、丹波卓也、A. E. Pegg、白幡晶
    • Organizer
      日本ポリアミン学会第2回年会
    • Place of Presentation
      宇都宮
    • Related Report
      2011 Final Research Report
  • [Presentation] Spermine synthase interacts with Glyceraldehyde-3-phosphate dehydrogenase and regulates its activity2010

    • Author(s)
      Ikeguchi, Y.
    • Organizer
      2010 INTERNATIONAL POLYAMINE CONFERENCE
    • Place of Presentation
      静岡
    • Year and Date
      2010-06-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] ポリアミンの腸管上皮細胞における炎症性サイトカイン産生への影響2010

    • Author(s)
      渡辺啓介
    • Organizer
      日本薬学会 第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] N-アシル転移酵素反応に伴うアミン修飾化合物の生成2010

    • Author(s)
      平松富都志
    • Organizer
      日本薬学会 第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] 食事中ポリアミンの腸管機能修飾を伴う経口免疫寛容への関与2010

    • Author(s)
      桑原涼子
    • Organizer
      日本薬学会 第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] ポリアミンアナログによる水溶性高分子モデル化合物の腸管吸収への影響2010

    • Author(s)
      平樂華菜
    • Organizer
      日本薬学会 第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] ラット腸管におけるスペルミンの吸収促進作用機構の解明2010

    • Author(s)
      山田真史
    • Organizer
      日本薬学会 第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Spermine synthase interacts with Glyceraldehyde-3-phosphate dehydrogenase and regulates its activity2010

    • Author(s)
      Yoshihiko Ikeguchi, Michiko Kanuma, Ikumi Ishii, Sachiko Takahashi, Kenichi Yamazaki, Anthony E. Pegg and Akira Shirahata
    • Organizer
      2010 INTERNATIONAL POLYAMINE CONFERENCE
    • Place of Presentation
      御殿
    • Related Report
      2011 Final Research Report
  • [Presentation] 筋肉細胞におけるポリアミンの脂質蓄積に及ぼす影響2010

    • Author(s)
      上杉粋世, 石井育美, 坂口花織, 山嵜健一, 池口文彦, 白幡晶
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Related Report
      2011 Final Research Report
  • [Presentation] エテノ蛍光誘導体化法を用いるS-アデノシルメチオニン脱炭酸化酵素活性測定法の開発2010

    • Author(s)
      山嵜健一, 丹波卓也, 池口文彦, 白幡晶
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Related Report
      2011 Final Research Report
  • [Presentation] 疎水性化合物のスペルミン酸化酵素活性に及ぼす影響2010

    • Author(s)
      渡邉巧, 池口文彦, 山嵜健一, 五十嵐一衛, 白幡晶
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Related Report
      2011 Final Research Report
  • [Presentation] HepG2細胞における脂質蓄積に及ぼすポリアミンの影響2010

    • Author(s)
      岩城孝宏, 石井育美, 山嵜健一, 池口彦, 白幡晶
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Related Report
      2011 Final Research Report
  • [Presentation] Spermine SynthaseとGlyceraldehyde-3-phosphate dehydrogenaseとの相互作用解析2010

    • Author(s)
      神沼路子, 池口文彦, 高橋幸子, 山嵜健一, 白幡晶
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト皮膚線維芽細胞におけるポリアミンの薬物取り込みに及ぼす影響2010

    • Author(s)
      橋本崇, 池口文彦, 山嵜健一, 藤堂浩明, 杉林堅次, 白幡晶
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Related Report
      2011 Final Research Report
  • [Presentation] ポリアミンの神経細胞モデルPC12の分化および細胞応答に及ぼす影響2010

    • Author(s)
      猿谷幸展, 田代大介, 池口文彦, 田中亨, 山嵜健一, 岡崎真理, 谷覺, 日比野康英, 白幡晶
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Related Report
      2011 Final Research Report
  • [Presentation] SH標識量に基づくタンパク質のコンホメーション変化のMALDI-TOF MS検出法2010

    • Author(s)
      山口直紀, 住吉亜由里, 上田登茂子, 山嵜健一, 池口文彦, 白幡晶
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Related Report
      2011 Final Research Report
  • [Presentation] 蛍光基質を用いるHPLC法によるデオキシハイプシン合成酵素活性測定法の開発と応用2010

    • Author(s)
      日下部真彦, 池口文彦, 金子宜令, 渡邊亮介, 山嵜健一, 五十嵐一衛, 白幡晶
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Related Report
      2011 Final Research Report
  • [Presentation] HepG2細胞の脂質蓄積におけるポリアミンの役割の解明2009

    • Author(s)
      岩城孝宏, 石井育美, 山嵜健一, 池口文彦, 白幡晶
    • Organizer
      第53回日本薬学会関東支部会
    • Place of Presentation
      埼玉
    • Related Report
      2011 Final Research Report
  • [Presentation] Spermine Synthaseと相互作用を示すタンパク質の探索2009

    • Author(s)
      神沼路子, 池口文彦, 高橋幸子, 山嵜健一, 白幡晶
    • Organizer
      第3回トランスグルタミナーゼ研究会 & ポリアミン研究会合同学術集会(同時開催)第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] ポリアミン合成酵素阻害剤による脂質代謝への影響2009

    • Author(s)
      石井育美、池口文彦、山嵜健一、和田政裕、真野博、白幡晶
    • Organizer
      第3回トランスグルタミナーゼ研究会 & ポリアミン研究会合同学術集会(同時開催)第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] Polyamine analogs affect neurite outgrowth and cellular response of PC12 cells2009

    • Author(s)
      Ikeguchi, Y., Tashiro, D., Okazaki, M., Tani, S., Tanaka, T., Hibino, Y. and Shirahata, A.
    • Organizer
      Gordon Research Conference on Polyamines 2009
    • Place of Presentation
      米国
    • Related Report
      2011 Final Research Report
  • [Presentation] Effect of Polyamine Synthase Inhibitor on Adipogenesis of 3T3-L1 cells2009

    • Author(s)
      Ishii, I., Ikeguchi, Y., Yamazaki, K., Wada, M., Mano, H., Pegg, A. E. and Shirahata, A.
    • Organizer
      2009 Gordon Research Seminar on Polyamines及びGordon Research Conference on Polyamines
    • Place of Presentation
      米国
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.josai.ac.jp/~facpharm/nourish/yv/laboratory_of_cellular_physiology.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.josai.ac.jp/~facpharm/pharma6/yy/bio-analytical_chemistry.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.josai.ac.jp/~facpharm/nourish/yv/laboratory_of_cellular_physiology.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.josai.ac.jp/~facpharm/pharma6/yy/biochemistry.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.josai.ac.jp/~facpharm/nourish/yv/laboratory_of_cellular_physiology.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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