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Identification of endo-type chondroitin sulfate hydrolases applicable to neuronal regenerative therapy

Research Project

Project/Area Number 21590057
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionHokkaido University

Principal Investigator

YAMADA Shuhei  北海道大学, 大学院・先端生命科学研究院, 准教授 (70240017)

Co-Investigator(Renkei-kenkyūsha) SUGAHARA Kazuyuki  北海道大学, 大学院・先端生命科学研究院, 教授 (60154449)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsグリコサミノグリカン / コンドロイチン硫酸 / ヒアルロン酸 / 神経再生 / ヒアル / ヒアルロニダーゼ
Research Abstract

In this study, human hyaluronidase-4 (HYAL4) was demonstrated to be a chondroitin sulfate (CS)-specific endo-type hydrolase for the first time. Although mouse hyaluronidase-4 (mHyal4) was also demonstrated to be a CS-specific endo-type hydrolase, it has broader substrate specificity than HYAL4. A series of human/mouse hyaluronidase-4 chimeric proteins and point mutants were generated, and the amino acid residues responsible for the difference in substrate specificity were identified. To prepare an active enzyme applicable to neuronal regenerative therapy in a large scale, the expression system in yeast was constructed. Injury region was extracted from the rats with lateral hemisection of the spinal cord, and the composition of CS in the region as well as its sensitivity to mHyal4 was investigated.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (85 results)

All 2012 2011 2010 2009

All Journal Article (24 results) (of which Peer Reviewed: 23 results) Presentation (56 results) Book (1 results) Patent(Industrial Property Rights) (4 results)

  • [Journal Article] 新規のコンドロイチン硫酸に特異的な加水分解酵素2012

    • Author(s)
      山田修平
    • Journal Title

      応用糖質科学

      Volume: (印刷中)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Demonstration of the Hepatocyte Growth Factor Signaling Pathway in the in vitro Neuritogenic Activity of Chondroitin Sulfate from Ray Fish Cartilage2011

    • Author(s)
      Taishi Hashiguchi, Takanari Kobayashi, Duriya Fongmoon, Ajaya Kumar Shetty, Shuji Mizumoto, Nobuyuki Miyamoto, Toshikazu Nakamura, Shuhei Yamada#, and Kazuyuki Sugahara#
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1810(4) Pages: 406-413

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evolution of Glycosaminoglycans: Comparative Biochemical Study2011

    • Author(s)
      Shuhei Yamada#, Kazuyuki Sugahara, and Suat Ozbek
    • Journal Title

      Commun. Integr. Biol

      Volume: 4(2) Pages: 150-158

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Unique heparan sulfate from shrimp heads exhibits a strong inhibitory effect on infections by dengue virus and Japanese encephalitis virus2011

    • Author(s)
      Jiancheng Chen, Shuhei Yamada#, Yoshiki Hama, Ajaya Kumar Shetty, Takanari Kobayashi, Hiroshi Oda, Kosuke Seiki, Eunmi Kim, Takashi Kimura, Naonori Takahashi, Kazuya I. P. J. Hidari, Takashi Suzuki, Yasuo Suzuki, and Kazuyuki Sugahara#
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 412(1) Pages: 136-142

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of HNK-1 sulfotransferase in the biosynthesis of the GlcUA(3-O-sulfate)-Gal-Gal-Xyl tetrasaccharide found in ?-thrombomodulin from human urine2011

    • Author(s)
      Taishi Hashiguchi, Shuji Mizumoto, Yuko Nishimura, Jun-ichi Tamura, Shuhei Yamada#, and Kazuyuki Sugahara#
    • Journal Title

      J. Biol. Chem

      Volume: 286(38) Pages: 33003-33011

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of HNK-1 sulfotransferase in the biosynthesis of chondro itin sulfate and the GlcA(3-O-sulfate)-Gal-Gal-Xyl tetrasaccharide found in α-thrombomodulin from human urine2011

    • Author(s)
      Taishi Hashiguchi
    • Journal Title

      J.Biol.Chem

      Volume: 286 Issue: 38 Pages: 33003-33011

    • DOI

      10.1074/jbc.m111.279174

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unique heparan sulfate from shrimp heads exhibits a strong inhibitory effect on infections by dengue virus and Japanese encephalitis virus2011

    • Author(s)
      Jiancheng Chen
    • Journal Title

      Biochem.Biophys.Res.Commun

      Volume: 412 Issue: 1 Pages: 136-142

    • DOI

      10.1016/j.bbrc.2011.07.059

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Demonstration of the hepatocyte growth factor signaling pathway in the in vitro neuritogenic activity of chondroitin sulfate from ray fish cartilage2011

    • Author(s)
      Taishi Hashiguchi
    • Journal Title

      Bionchim.Biophys.Acta

      Volume: 1810 Issue: 4 Pages: 406-413

    • DOI

      10.1016/j.bbagen.2011.01.001

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biochim. Biophys. Acta2011

    • Author(s)
      Taishi Hashiguchi
    • Journal Title

      Demonstration of the hepatocyte growth factor signaling pathway in the in vitro neuritogenic activity of chondroitin sulfate from ray fish cartilage.

      Volume: 1810 Pages: 406-413

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evolution of glycosaminoglycans: comparative biochemical study.2011

    • Author(s)
      Shuhei Yamada
    • Journal Title

      Commun. Integr. Biol.

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of Human Hyaluronidase-4 as a Novel Chondroitin Sulfate Hydrolase That Preferentially Cleaves the Galactosaminidic Linkage in the Trisulfated Tetrasaccharide Sequence2010

    • Author(s)
      Tomoyuki Kaneiwa, Shuji Mizumoto, Kazuyuki Sugahara, and Shuhei Yamada#
    • Journal Title

      Glycobiology

      Volume: 20(3) Pages: 300-309

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a Mouse Monoclonal Antibody Against the Chondroitin Sulfate-protein Linkage Region Derived from Shark Cartilage2010

    • Author(s)
      Chizuru Akatsu, Duriya Fongmoon, Shuji Mizumoto, Jean-Claude Jacquinet, Prachya Kongtawelert, Shuhei Yamada#, and Kazuyuki Sugahara#
    • Journal Title

      Glycoconjugate J

      Volume: 27(4) Pages: 387-399

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a mouse monoclonal antibody against the chondroitin sulfate-protein linkage region derived from shark cartilage.2010

    • Author(s)
      Chizuru Akatsu
    • Journal Title

      Glycoconjugate J.

      Volume: 27 Pages: 387-399

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of human hyaluronidase-4 as a novel chondroitin sulfate hydrolase that preferentially cleaves the galactosaminidic linkage in the trisulfated tetrasaccharide sequence.2010

    • Author(s)
      Tomoyuki Kaneiwa
    • Journal Title

      Glycobiology 20

      Pages: 300-309

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a mouse monoclonal antibody against the chondroitin sulfate-protein linkage region derived from shark cartilage.2010

    • Author(s)
      Chizuru Akatsu
    • Journal Title

      Glycoconjugate Journal (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Important Role of Heparan Sulfate in Postnatal Islet Growth and Insulin Secretion2009

    • Author(s)
      Iwao Takahashi, Naoya Noguchi, Koji Nata, Shuhei Yamada, Tomoyuki Kaneiwa, Shuji Mizumoto, Takayuki Ikeda, Kazushi Sugihara, Masahide Asano, Takeo Yoshikawa, Akiyo Yamauchi, Nausheen Jamal Shervani, Akira Uruno, Ichiro Kato, Michiaki Unno, Kazuyuki Sugahara, Shin Takasawa, Hiroshi Okamoto, and Akira Sugawara
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 383(1) Pages: 113-118

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and Characterization of a Novel Chondroitin Sulfate from Squid LiverIntegument Rich in N-Acetylgalactosamine(4, 6-O-disulfate) and Glucuronate(3-O-sulfate) Residues2009

    • Author(s)
      Ajaya Kumar Shetty, Takanari Kobayashi, Shuji Mizumoto, Masaki Narumi, YoshiakiKudo, Shuhei Yamada#, and Kazuyuki Sugahara#
    • Journal Title

      Carbohydr. Res

      Volume: 344(12) Pages: 1526-1532

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural and Functional Changes of Sulfated Glycosaminoglycans in Xenopus laevis during Embryogenesis2009

    • Author(s)
      huhei Yamada#, Masako Onishi, Reiko Fujinawa, Yuko Tadokoro, Koji Okabayashi, Makoto Asashima, and Kazuyuki Sugahara
    • Journal Title

      Glycobiology

      Volume: 19(5) Pages: 488-498

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chondroitin Hydrolase in Caenorhabditis elegans2009

    • Author(s)
      huhei Yamada#, Shuji Mizumoto, and Kazuyuki Sugahara
    • Journal Title

      Trends Glycosci. Glycotechnol

      Volume: 21(119) Pages: 149-162

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Important role of heparan sulfate in postnatal islet growth and insulin secretion.2009

    • Author(s)
      Iwao Takahashi
    • Journal Title

      Biochem.Biophys.Res.Commun. 383

      Pages: 113-118

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4,6-O-disulfate)and glucuronate(3-O-sulfate)residues.2009

    • Author(s)
      Ajaya Kumar Shetty
    • Journal Title

      Carbohydr. Res. 344

      Pages: 1526-1532

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural and functional changes of sulfated glycosaminoglycans in Xenopus laevis during embryogenesis.2009

    • Author(s)
      Shuhei Yamada
    • Journal Title

      Glycobiology 19

      Pages: 488-498

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chondroitin Hydrolase in Caenorhabditis elegans.2009

    • Author(s)
      Shuhei Yamada
    • Journal Title

      Trends Glycosci. Glycotechnol. 21

      Pages: 149-162

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] グリコサミノグリカン-生体シグナル分子相互作用2009

    • Author(s)
      水本秀二
    • Journal Title

      遺伝子医学MOOK 別冊

      Pages: 223-230

    • Related Report
      2009 Annual Research Report
  • [Presentation] 哺乳類におけるコンドロイチン硫酸特異的加水分解酵素ヒアルロニダーゼ-4の基質認識に必要なアミノ酸残基の同定2012

    • Author(s)
      Shuhei Yamada
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] ユニークな構造と機能をもつエビ頭部に由来するヘパラン硫酸2012

    • Author(s)
      羽磨紀城
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒアルロニダーゼのコンドロイチン硫酸に対する加水分解活性の定量的解析2012

    • Author(s)
      本田智子
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] 「Shrimp head heparan sulfate with unique structure and function2012

    • Author(s)
      Yoshiki Hama, Chen Jiancheng, Ajaya Kumar Shetty, Takanari Kobayashi, Hiroshi Oda, Kosuke Seiki, Eunmi Kim, Takashi Kimura, Kazuyuki Sugahara, Shuhei Yamada
    • Organizer
      The IGP International Symposium 2012
    • Place of Presentation
      Sapporo
    • Year and Date
      2012-03-02
    • Related Report
      2011 Final Research Report
  • [Presentation] Shrimp head heparan sulfate with unique structure and function2012

    • Author(s)
      Yoshiki Hama
    • Organizer
      The IGP International Symposium 2012
    • Place of Presentation
      Hokkaido University (Sapporo)
    • Year and Date
      2012-03-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒアルロニダーゼのコンドロイチン硫酸加水分解活性の定量2011

    • Author(s)
      本田智子
    • Organizer
      日本薬学会北海道支部第136回例会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2011-12-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] 「Involvement of HNK-1 Sulfotransferase in the Biosynthesis of Chondroitin Sulfate and the GlcUA(3-O-sulfate)-Gal-Gal-Xyl Tetrasaccharide Found in ?-Thrombomodulin from Human Urine」2011

    • Author(s)
      T. Hashiguchi, S. Mizumoto, Y. Nishimura, J. Tamura, S. Yamada, K. Sugahara
    • Organizer
      7th International Conference onProteoglycans
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      2011-10-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Involvement of HNK-1 Sulfotransferase in the Biosynthesis of Chondroitin Sulfate and the GlcUA(3-O-sulfate)-Gal-Gal-Xyl Tetrasaccharide Found in a-Thrombomodulin from Human Urine2011

    • Author(s)
      Shuhei Yamada
    • Organizer
      7th International Conference on Proteoglycans
    • Place of Presentation
      QSTATION (Australia)
    • Year and Date
      2011-10-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Determination of amino acid sequences in hyaluronidase-4 responsible for the substrate recognition2011

    • Author(s)
      T. Kaneiwa, A. Miyazaki, S. Mizumoto, K. Sugahara, and S. Yamada
    • Organizer
      7th International Conference on Proteoglycans
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      2011-10-18
    • Related Report
      2011 Final Research Report
  • [Presentation] Determination of amino acid sequences in hyaluronidase-4 responsible for the substrate recognition2011

    • Author(s)
      Tomoyuki Kaneiwa
    • Organizer
      7th International Conference on Proteoglycans.
    • Place of Presentation
      QSTATION (Australia)
    • Year and Date
      2011-10-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] 新規のコンドロイチン硫酸に特異的な加水分解酵素の同定2011

    • Author(s)
      山田修平
    • Organizer
      第19回糖質関連酵素化学シンポジウム
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2011-09-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Specific interaction of the envelope glycoproteins E1 and E2 with liver heparan sulfate involved in the tissue tropismatic infection by hepatitis C virus2011

    • Author(s)
      Shuhei Yamada
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒトヒアルロニダーゼのコンドロイチン硫酸に対する作用の定量的解析2011

    • Author(s)
      本田智子
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] エビ頭部由来ヘパラン硫酸の構造と機能の研究2011

    • Author(s)
      羽磨紀城
    • Organizer
      日本薬学会北海道支部第136回例会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2011-05-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] Identification of chondroitin sulfate-specific novel hydrolases.2011

    • Author(s)
      Tomoyuki Kaneiwa
    • Organizer
      International Fusion-Bioscience Symposium for young researchers
    • Place of Presentation
      Sapporo
    • Year and Date
      2011-03-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Mouse hyaluronidase 4:identification of a chondroitin sulfate-specific hydrolase activity.2011

    • Author(s)
      Anzu Miyazaki
    • Organizer
      International Fusion-Bioscience Symposium for young researchers
    • Place of Presentation
      Sapporo
    • Year and Date
      2011-03-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Chondroitin sulfate-specific novel hydrolase in human2011

    • Author(s)
      山田修平
    • Organizer
      9th International Symposium on Biochemical Roles of Eukaryotic Cell Surface Macromolecules
    • Place of Presentation
      Trivandrum, Kerala, India
    • Year and Date
      2011-01-28
    • Related Report
      2011 Final Research Report
  • [Presentation] Chondroitin sulfate-specific novel hydrolase in human.2011

    • Author(s)
      Shuhei Yamada
    • Organizer
      9th International Symposium on Biochemical Roles of Eukaryotic Cell Surface Macromolecules
    • Place of Presentation
      Trivandrum, Kerala, India(招待講演)
    • Year and Date
      2011-01-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Mouse hyaluronidase-4 : Identification of a chondroitin sulfate-specific hydrolase with distinct specificity from human hyaluronidase-42011

    • Author(s)
      S. Yamada, A. Miyazaki, T. Kaneiwa, S. Mizumoto, and K. Sugahara
    • Organizer
      The 31st Naito Conference. Glycan Expression and Regulation [II]: Metabolites, Stress Response, Microdomains, and Beyond.
    • Place of Presentation
      Sapporo
    • Related Report
      2011 Final Research Report
  • [Presentation] Studies on novel mammalian endo-type chondroitin sulfate hydrolases2011

    • Author(s)
      T. Kaneiwa, A. Miyazaki, S. Mizumoto, K. Sugahara, S. Yamada
    • Organizer
      The 3rd FEBS-MPST Advanced Lecture Course on Matrix Pathobiology, Signaling andMolecular Targets (FEBS-MPST2011).
    • Place of Presentation
      Spetses, Greece
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒトヒアルロニダーゼ4とマウスヒアルロニダーゼ4の基質特異性の比較2011

    • Author(s)
      山田修平
    • Organizer
      第30回日本糖質学会年会
    • Place of Presentation
      リリックホール・ハイブ長岡(長岡市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] エビ頭部に由来するヘパラン硫酸の構造と生物活性2011

    • Author(s)
      羽磨紀城
    • Organizer
      第30回日本糖質学会年会
    • Place of Presentation
      リリックホール・ハイブ長岡(長岡市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Studies on novel mammalian endo-type chondroitin sulfate hydrolases2011

    • Author(s)
      Tomoyuki Kaneiwa
    • Organizer
      The 3rd FEBS-MPST Advanced Lecture Course on Matrix Pathobiology, Signaling and Molecul ar Targets (FEBS-MPST2011)
    • Place of Presentation
      Anargyrios & Korgialenios School (Greece)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Mouse hyaluronidase-4 : Identification of a chondroitin sulfate-specific hydrolase with distinct specificity from human hyaluronidase-42011

    • Author(s)
      Shuhei Yamada
    • Organizer
      The 31st Naito Conference. Glycan Expression and Regulation [II] : Metabolites, Stress Response, Microdomains, Beyond
    • Place of Presentation
      CHATERAISE Gateaux Kingdom (Sapporo)
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒトにおける新規のコンドロイチン硫酸特異的加水分解酵素の同定2010

    • Author(s)
      金岩知之
    • Organizer
      BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] コンドロイチン硫酸と芯タンパク質の結合領域に対するモノクローナル抗体の開発とその反応性の解析2010

    • Author(s)
      山田修平
    • Organizer
      BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウスヒアルロニダーゼ4:コンドロイチン硫酸特異的加水分解酵素の同定2010

    • Author(s)
      宮崎杏
    • Organizer
      BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification of a novel chondroitin sulfate hydrolase applicable to neuronal regenerative therapy2010

    • Author(s)
      Trivandrum, Kerala, IndiaT. Kaneiwa, S. Mizumoto, K. Sugahara, S. Yamada
    • Organizer
      The 8th International Symposium for Future Drug Discovery and Medical Care
    • Place of Presentation
      Sapporo
    • Year and Date
      2010-09-02
    • Related Report
      2011 Final Research Report
  • [Presentation] Identification of a novel chondroitin sulfate hydrolase applicable to neuronal regeneranve theray.2010

    • Author(s)
      Tomoyuki Kaneiwa
    • Organizer
      The 8th International Symposium for Future Drug Discovery and Medical Care
    • Place of Presentation
      Sapporo
    • Year and Date
      2010-09-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification of a chondroitin sulfate-specific novel hydrolase in human2010

    • Author(s)
      T. Kaneiwa, S. Mizumoto, K. Sugahara, S. Yamada
    • Organizer
      The 25th International Carbohydrate Symposium (ICS2010)
    • Place of Presentation
      Chiba
    • Year and Date
      2010-08-05
    • Related Report
      2011 Final Research Report
  • [Presentation] Identification of a chondroitin sulfate-specific novel hydrolase in human.2010

    • Author(s)
      Tomoyuki Kaneiwa
    • Organizer
      The 25th International Carbohydrate Symposium (ICS2010)
    • Place of Presentation
      Chiba
    • Year and Date
      2010-08-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] Involvement of the Hepatocyte Growth Factor Signaling Pathway in the Neuritogenic Activity of Chondroitin Sulfate Derived from Ray Fish Cartilage2010

    • Author(s)
      T. Hashiguchi, T. Kobayashi, D. Fongmoon, A. K. Shetty, S. Mizumoto, N. Miyamoto, T. Nakamura, S. Yamada, and K. Sugahara
    • Organizer
      The 25th International Carbohydrate Symposium (ICS2010)
    • Place of Presentation
      Chiba
    • Year and Date
      2010-08-03
    • Related Report
      2011 Final Research Report
  • [Presentation] Involvement of the hepatocyte growth factor signaling pathway in the neuritogenic activity of chondroitin sulfate derived from ray fish cartilage.2010

    • Author(s)
      Taishi Hashiguchi
    • Organizer
      The 25th International Carbohydrate Symposium (ICS2010)
    • Place of Presentation
      Chiba
    • Year and Date
      2010-08-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Structural characterization of heparan sulfate in the Extl3 knockout mice and the pancreatic β-cell specific Extl3 knockout mice2010

    • Author(s)
      T. Kaneiwa, S. Yamada, K. Sugahara, I. Takahashi, N. Noguchi, K. Nata, H. Okamoto, A. Sugawara
    • Organizer
      The 25th International Carbohydrate Symposium (ICS2010)
    • Place of Presentation
      Chiba
    • Year and Date
      2010-08-02
    • Related Report
      2011 Final Research Report
  • [Presentation] Structural characterization of heparan sulfate in the Extl3 knockout mice and the pancreatic β-cell specific Extl3 knockout mice.2010

    • Author(s)
      Shuhei Yamada
    • Organizer
      The 25th International Carbohydrate Symposium (ICS2010)
    • Place of Presentation
      Chiba
    • Year and Date
      2010-08-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウスにおけるコンドロイチン硫酸特異的加水分解酵素の同定2010

    • Author(s)
      宮崎杏
    • Organizer
      第47回日本生化学会北海道支部例会
    • Place of Presentation
      札幌
    • Year and Date
      2010-07-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] コンドロイチン硫酸のコアタンパク質への結合領域に対する単クローン抗体の開発とその反応性の解析2010

    • Author(s)
      赤津ちつる
    • Organizer
      第47回日本生化学会北海道支部例会
    • Place of Presentation
      札幌
    • Year and Date
      2010-07-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] エイ軟骨由来コンドロイチン硫酸は肝細胞増殖因子を介してマウス海馬神経細胞の突起を伸長させる2010

    • Author(s)
      橋口太志
    • Organizer
      日本薬学会北海道支部第134回例会
    • Place of Presentation
      札幌
    • Year and Date
      2010-05-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウスの各組織におけるコンドロイチン硫酸(CS)/デルマタン硫酸(DS)の硫酸化パターンの解析2010

    • Author(s)
      宮崎杏
    • Organizer
      日本薬学会北海道支部第134回例会
    • Place of Presentation
      札幌
    • Year and Date
      2010-05-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒアルロニダーゼ4:新規コンドロイチン硫酸特異的エンド型加水分解酵素の同定2010

    • Author(s)
      金岩知之
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] エイ軟骨由来コンドロイチン硫酸の構造および生理活性の解析2010

    • Author(s)
      橋口太志
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Demonstration of chondroitin sulfate-specific hydrolytic activity in human hyaluronidase-42010

    • Author(s)
      T. Kaneiwa, S. Mizumoto, K. Sugahara, S. Yamada
    • Organizer
      The 8th International Conference on Hyaluronan Sciences
    • Place of Presentation
      Kyoto
    • Related Report
      2011 Final Research Report
  • [Presentation] Demonstration of chondroitin sulfate-specific hydrolytic activity in human hyaluronidase-4.2010

    • Author(s)
      Tomoyuki Kaneiwa
    • Organizer
      The 8th International Conference on Hyaluronan Sciences
    • Place of Presentation
      Kyoto
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification of Human Hyaluronidase-4 as a Novel Chondroitin Sulfate Hydrolase That Preferentially Cleaves the Specific Linkage in a Highly Sulfated Sequence2009

    • Author(s)
      Shuhei Yamada, Tomoyuki Kaneiwa, Shuji Mizumoto, and Kazuyuki Sugahara
    • Organizer
      6th International Conference on Proteoglycan
    • Place of Presentation
      Aix-les-Bains, France
    • Year and Date
      2009-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] Identification of human hyaluronidase-4 as a novel chondroitin sulfate hydrolase that preferentially cleaves the specific linkage in a highly sulfated sequence.2009

    • Author(s)
      Shuhei Yamada
    • Organizer
      6th international Conference on Proteoglycan
    • Place of Presentation
      Aix-les-Bains, France
    • Year and Date
      2009-09-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] Extl3 KOマウス及び膵B細胞特異的なExtl3 KOマウスのヘパラン硫酸の分析2009

    • Author(s)
      山田修平
    • Organizer
      第29回日本糖質学会年会
    • Place of Presentation
      高山
    • Year and Date
      2009-09-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] コンドロイチン硫酸に特異的な新規のエンド型加水分解酵素の同定2009

    • Author(s)
      金岩知之
    • Organizer
      シオノギイノベーションフェア2009
    • Place of Presentation
      札幌
    • Year and Date
      2009-08-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] エイ軟骨由来コンドロイチン硫酸の神経突起伸長促進活性の解析2009

    • Author(s)
      長嶋眞也
    • Organizer
      第3回GFRG(Glycoform-focused reverse genomics)研究会公開シンポジウム
    • Place of Presentation
      札幌
    • Year and Date
      2009-08-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] Identification and characterization of a novel chondroitin hydrolase in Caenorhabditis elegans2009

    • Author(s)
      Tomoyuki Kaneiwa, Shuhei Yamada, Shuji Mizumoto, Adriana M. Montano, Shohei Mitani, and Kazuyuki Sugahara
    • Organizer
      2nd Symposium on Structural Analysis of Biological Macromolecules & 7th Japan-Korea Bilateral Symposium on Biological NMR
    • Place of Presentation
      Sapporo
    • Year and Date
      2009-07-27
    • Related Report
      2011 Final Research Report
  • [Presentation] Structure and biological activities of heparin-like glycosaminoglycan from shrimp heads2009

    • Author(s)
      Jiancheng Chen, Ajaya Kumar Shetty, Takanari Kobayashi, Hiroshi Oda, Kosuke Seiki, Shuhei Yamada, and Kazuyuki Sugahara
    • Organizer
      2nd Symposium on Structural Analysis of Biological Macromolecules & 7th Japan-Korea Bilateral Symposium on Biological NMR
    • Place of Presentation
      Sapporo
    • Year and Date
      2009-07-27
    • Related Report
      2011 Final Research Report
  • [Presentation] Identification and characterization of a novel chondroitin hydrolase in Caenorhabditis elegans.2009

    • Author(s)
      Tomoyuki Kaneiwa
    • Organizer
      2^<nd> Symposium on Structural Analysis of Biological Macromolecules & 7^<th> Japan-Korea Bilateral Symposium on Biological NMR
    • Place of Presentation
      Sapporo
    • Year and Date
      2009-07-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] エイ軟骨由来コンドロイチン硫酸の構造および生理活性の解析2009

    • Author(s)
      長嶋眞也
    • Organizer
      日本生化学会北海道支部第46回例会
    • Place of Presentation
      旭川
    • Year and Date
      2009-07-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] コンドロイチン硫酸のコアタンパク質への結合領域に対するマウスモノクローナル抗体の作製とそのエピトープの解析2009

    • Author(s)
      赤津ちづる
    • Organizer
      日本薬学会北海道支部第132回例会
    • Place of Presentation
      札幌
    • Year and Date
      2009-05-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Structural characterization of heparan sulfate in conventional and conditional Extl3-deficient mice.2009

    • Author(s)
      Tomoyuki Kaneiwa
    • Organizer
      Hokkaido University-Mahidol University Joint Symposium
    • Place of Presentation
      Sapporo
    • Year and Date
      2009-05-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] Identification and characterization of a novel chondroitin hydrolase in Caenorhabditis elegans2009

    • Author(s)
      Tomoyuki Kaneiwa, Shuhei Yamada, Shuji Mizumoto, Adriana M. Montano, Muneyoshi Otori, Shohei Mitani, and Kazuyuki Sugahara
    • Organizer
      6th International Conference on Proteoglycan
    • Place of Presentation
      Aix-les-Bains, France
    • Related Report
      2011 Final Research Report
  • [Presentation] Identification and characterization of a novel chondroitin hydrolase in Caenorhabditis elegans.2009

    • Author(s)
      Tomoyuki Kaneiwa
    • Organizer
      6th international Conference on Proteoglycan
    • Place of Presentation
      Aix-les-Bains, France
    • Related Report
      2009 Annual Research Report
  • [Book] 遺伝子医学MOOK別冊ますます重要になる細胞周辺環境(細胞ニッチ)の最新科学技術 .細胞の生存、増殖、機能のコントロールから創薬研究、再生医療まで.2009

    • Author(s)
      水本秀二,山田修平,菅原一幸
    • Total Pages
      230
    • Publisher
      メディカルドゥ
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] コンドロイチン硫酸オリゴ糖の製造方法2012

    • Inventor(s)
      松嶋景一郎,浦晴雄,鎌田樹志,宮本宜之,菅原一幸,山田修平
    • Industrial Property Rights Holder
      地方独立行政法人北海道立総合研究機構,丸共水産株式会社,国立大学法人北海道大学
    • Industrial Property Number
      2012-035546
    • Filing Date
      2012-02-21
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] コンドロイチン硫酸オリゴ糖の製造方法2012

    • Inventor(s)
      松嶋景一郎, 浦晴雄, 鎌田樹志, 宮本宜之, 菅原一幸, 山田修平
    • Industrial Property Rights Holder
      地方独立行政法人北海道立総合研究機構, 丸共水産株式会社, 国立大学法人北海道大学
    • Industrial Property Number
      2012-035546
    • Filing Date
      2012-02-21
    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] グリコサミノグリカン分解促進剤2009

    • Inventor(s)
      菅原一幸,山田修平,水本秀二,金岩知之
    • Industrial Property Rights Holder
      国立大学法人北海道大学
    • Industrial Property Number
      2009-238490
    • Filing Date
      2009-10-15
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] グリコサミノグリカン分解促進剤2009

    • Inventor(s)
      菅原一幸, 山田修平, 水本秀二, 金岩知之
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Number
      2009-238490
    • Filing Date
      2009-10-15
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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