Analysis on the function and coalition of RecQL4, a product of a gene mutated in a progeria syndrome, in the DNA transactions.

• Project/Area Number: 21590059
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Teikyo Heisei University (2010-2011); Tohoku University (2009)
• Principal Investigator: TADA Shusuke 帝京平成大学, 薬学部, 教授 (00216970)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: Rothmund-Thomson症候群 / RecQL4 / DNA修復 / DNA複製 / 遺伝子構造安定性維持 / 遺伝情報安定性維持機構 / 質量分析 / ユビキチン化 / ニワトリDT40細胞

Research Abstract

In this study, we aimed to understand the function and behavior of RecQL4, a product of a gene mutated in a progeria syndrome, in higher eukaryotic cells. Cell-biological or biochemical analyses of RecQL4 revealed that 1) RecQL4 is promptly translocated onto a site of DNA lesions, 2) RecQL4 interacts with numbers of post-translational modification enzymes, as well as proteins relevant to recognition and repair of DNA lesions, and 3) RecQL4 possesses domains for viability of higher eukaryotic cellsand for coping with DNA damagesin separate regions.

Research Products

• [Journal Article] The roles of histone chaperone CIA/Asf1 in nascent DNA elongation during nucleosome replication. (2011)
  • Author(s): K.Ishikawa, T.Ohsumi, S.Tada, R.Natsume, L.R.Kundu, N.Nozaki, T.Senda, T.Enomoto, M.Horikoshi & M.Seki
  • Journal Title: Genes Cells Volume: 16 Issue: 10 Pages: 1050-1062
  • DOI: 10.1111/j.1365-2443.2011.01549.x
  • Peer Reviewed
• [Journal Article] Functional relationship between Claspin and Rad17 (2011)
  • Author(s): Yoshimura, A., Akita, M., Hosono, Y., Abe, T., Kobayashi, M., Yamamoto, K.I., Tada, S., Seki, M., Enomoto, T.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 414 Issue: 2 Pages: 298-303
  • DOI: 10.1016/j.bbrc.2011.09.037
  • Peer Reviewed
• [Journal Article] The histone chaperone FACT maintains replication fork rates (2011)
  • Author(s): T.Abe, K.Sugimura, Y.Hosono, Y.Takami, M.Akita, A.Yoshimura, S.Tada, T.Nakayama, H.Murofushi, K.Okumura, S.Takeda, M.Horikoshi, M.Seki & T.Enomoto
  • Journal Title: J.Biol.Chem. Volume: 286 Issue: 35 Pages: 30504-30512
  • DOI: 10.1074/jbc.m111.264721
  • Peer Reviewed
• [Journal Article] Biphasic chromatin binding of histone chaperone FACT during eukaryotic chromatin DNA replication (2011)
  • Author(s): Kundu L. R., Seki M., Watanabe N., Murofushi H., Furukohri A., Waga S., Score A. J., Blow J. J., Horikoshi M., Enomoto T., Tada S.
  • Journal Title: Biochim. Biophys. Acta Volume: 1813 Pages: 1129-1136
  • Peer Reviewed
• [Journal Article] The N-terminal region of RECQL4 lacking the helicase domain is both essential and sufficient for the viability of vertebrate cells (2011)
  • Author(s): Abe, T., Yoshimura, A., Hosono, Y., Tada, S., Seki, M., Enomoto. T
  • Journal Title: Biochim.Biophys.Acta Volume: 1813 Issue: 3 Pages: 473-479
  • DOI: 10.1016/j.bbamcr.2011.01.001
  • Peer Reviewed
• [Journal Article] Werner Interacting Protein 1 Promotes Binding of Werner Protein to Template-Primer DNA (2011)
  • Author(s): Kanamori M, Seki, M, Yoshimura A, Tsurimoto T, Tada S, Enomoto T
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 34 Issue: 8 Pages: 1314-1318
  • DOI: 10.1248/bpb.34.1314
  • NAID: 130000936605
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] Functional relationship between Claspin and Rad17 (2011)
  • Author(s): Akari Yoshimura, et al
  • Journal Title: Biochemical and Biophysical Research Communication Volume: 414 Pages: 298-303
  • Peer Reviewed
• [Journal Article] The N-terminal region of RECQL4 lacking the helicase domain is both essential and sufficient for the viability of vertebrate cells. Role of the N-terminal region of RECQL4 in cells. (2011)
  • Author(s): Takuya Abe, et al.
  • Journal Title: Biochimica et Biophysica Acta Molecular Cell Research Volume: 1813 Pages: 473-479
  • Peer Reviewed
• [Journal Article] Biphasic chromatin binding of histone chaperone FACT during eukaryotic chromatin DNA replication. (2011)
  • Author(s): Lena R.Kundu, et al.
  • Journal Title: Biochimica et Biophysica Acta Molecular Cell Research Volume: 1813 Pages: 1129-1136
  • Peer Reviewed
• [Journal Article] SOD1 Is Essential for the Viability of DT40 Cells and Nuclear SOD1 Functions as a Guardian of Genomic DNA (2010)
  • Author(s): Inoue E., Tano K., Yoshii H., Nakamura J., Tada S., Watanabe M., Seki M., Enomoto T.
  • Journal Title: J Nucleic Acids Volume: 2010 Issue: 1 Pages: 795946-795946
  • DOI: 10.4061/2010/795946
  • Peer Reviewed
• [Journal Article] Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex (2010)
  • Author(s): Kundu L. R., Kumata Y., Kakusho N., Watanabe S., Furukohri A., Waga S., Seki M., Masai H., Enomoto T., Tada S.
  • Journal Title: Nucleic Acids Res Volume: 38 Issue: 16 Pages: 5409-5418
  • DOI: 10.1093/nar/gkq262
  • Peer Reviewed
• [Journal Article] Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex. (2010)
  • Author(s): Lena R.Kundu, et al.
  • Journal Title: Nucleic Acids Research Volume: 38 Pages: 5409-5418
  • Peer Reviewed
• [Journal Article] Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex (2010)
  • Author(s): Lena Rani Kundu, ほか
  • Journal Title: Nucleic Acids Research (印刷中)
  • Peer Reviewed
• [Journal Article] Structure of the Cdt1 C-terminal domain : Conservation of the winged helix fold in replication licensing factors (2009)
  • Author(s): Khayrutdinov, B. I., Bae, W. J., Yun, Y. M., Lee, J. H., Tsuyama, T., Kim, J. J., Hwang, E., Ryu, K. S., Cheong, H. K., Cheong, C., Ko, J. S., Enomoto, T., Karplus, P. A., Guntert, P., Tada, S., Jeon, Y. H., and Cho, Y.
  • Journal Title: Protein Sci Volume: 18 Issue: 11 Pages: 2252-2264
  • DOI: 10.1002/pro.236
  • Peer Reviewed
• [Journal Article] Repression of nascent strandelongation by deregulated Cdt1 during DNA replication in Xenopus egg extracts (2009)
  • Author(s): Tsuyama, T., Watanabe, S., Aoki, A., Cho, Y., Seki, M., Enomoto, T. and Tada, S.
  • Journal Title: Mol. Biol Volume: 20 Issue: 3 Pages: 937-947
  • DOI: 10.1091/mbc.e08-06-0613
  • Peer Reviewed
• [Journal Article] Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication (2009)
  • Author(s): Ohuchi, T., Seki, M., Kugou, K., Tada, S., Ohta, K. and Enomoto, T.
  • Journal Title: DNA Repair Volume: 8 Issue: 6 Pages: 690-696
  • DOI: 10.1016/j.dnarep.2009.01.018
• [Journal Article] Physical and functional interaction between WRNIP1 and RAD18 (2009)
  • Author(s): Yoshimura, A., Seki, M., Kanamori, M., Tateishi, S., Tsurimoto, T., Tada, S. and Enomoto, T.
  • Journal Title: Genes & Genetic Systems Volume: 84 Issue: 2 Pages: 171-178
  • DOI: 10.1266/ggs.84.171
  • NAID: 10024791852
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed
• [Journal Article] Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication. (2009)
  • Author(s): Takashi Ohuchi, ほか
  • Journal Title: DNA Repair (Amst) 8 Pages: 690-696
  • Peer Reviewed
• [Journal Article] Physical and functional interaction between WRNIP1 and RAD18 (2009)
  • Author(s): Akari Yoshimura, ほか
  • Journal Title: Genes & Genetic Systems 84 Pages: 171-178
  • Peer Reviewed
• [Presentation] The N-terminal region of RECQL4 lacking the helicase domain is both essential and sufficient for the viability of vertebrate cells (2010)
  • Author(s): 吉村明, ほか
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2010-12-08
• [Presentation] Biphasic chromatin binding of histone chaperone FACT during eukaryotic chromatin DNA replication (2010)
  • Author(s): Kundu, L. R., ほか
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2010-12-07
• [Presentation] DNAヘリカーゼRECQL5のDNA組換え・修復における機能の解析 (2010)
  • Author(s): 細野嘉史, ほか
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2010-12-07
• [Presentation] Biphasic chromatin binding of histone chaperone FACT during eukaryotic chromatin DNA replication (2010)
  • Author(s): Lena R Kundu, et al.
  • Organizer: 生化学会・分子生物学会・合同年会(BMB2010)
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2010-12-07
• [Presentation] A mutant protein of histone chaperone CIA/Asf1 defective in disrupting histone(H3-H4)_2 tetramer accelerates rate of DNA replication (2009)
  • Author(s): 大隅達也, ほか
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-12-11
• [Presentation] Analysis of DNA replication repression by excess Cdt1 (2009)
  • Author(s): 牛田磨理, ほか
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-12-10
• [Presentation] Nuclear superoxide may trigger spontaneous sister chromatid exchanges (2009)
  • Author(s): 井上絵里, ほか
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-12-09
• [Presentation] DNA複製に必須なCdc45の新規機能の解析 (2009)
  • Author(s): 大隅達也, ほか
  • Organizer: 第48回日本薬学会東北支部大会
  • Place of Presentation: 東北薬科大学(仙台市)
  • Year and Date: 2009-10-18
• [Presentation] The initiation of DNA replication is suppressed by Cdc6, a protein essential for the pre-replicative complex formation (2009)
  • Author(s): 多田周右, ほか
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-10-03
• [Presentation] The initiation of DNA replication is suppressed by Cdc6, a proteinessential for the pre-replicative complex formation. (2009)
  • Author(s): 多田周右, ほか
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2009-10-03