| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
In this grant, I explored functional aspects of N-glycolylneuraminic acid(Neu5Gc) in mouse lymphocytes to understand immunological function of sialic acid species modulation. Targeted disruption of CD22 and Neu5Gc-biosynthesis enzyme CMP-Neu5Ac hydroxylase(CMAH) resulted in the non-overlapping phenotypes in lymphocytes. Obtained data indicated that Neu5Gc, although that serves as high affinity glycan ligand for CD22, functions partly apart from CD22 ligand. This research results indicated that Neu5Gc has CD22-independent cellular autonomous function(s), that may not be limited in lymphocytes. Therefore, when mice were utilized as a model for human in biomedical research, it is strongly recommended to use cmah-null background to avoid confusion in the phenotypic analysis.
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