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GeneticanalysisofDNAstrandbreakrepairinhumancelIs

Research Project

Project/Area Number 21590066
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionYokohama City University

Principal Investigator

ADACHI Noritaka  横浜市立大学, 生命ナノシステム科学研究科, 教授 (30264675)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords遺伝子ノックアウト / DNA鎖切断 / エンドジョイニング / 組換え / NDA鎖切断
Research Abstract

In this study, we have generated a series of human cell mutants that lack one or two genes implicated in DNA strand break repair. These gene-knockout mutants allowed us to perform comprehensive geneticanalysis of human DNA repair genes, especially those involved in DNA double-strand break repair and genetic recombination.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (26 results)

All 2012 2010 2009 Other

All Journal Article (25 results) (of which Peer Reviewed: 8 results) Remarks (1 results)

  • [Journal Article] EDGF/pisindependent HIV-l repli cation demonstrates a role for HRP-2 and remains sensitive to inhibition by LEDGINs2012

    • Author(s)
      Schrijvers R, De Rijck J, Demeu lemeester J, Adachi N, Vets S, Ronen K, Christ F, Bushman FD, Debyser Z, Gt jsbers R.
    • Journal Title

      PLoS Pathog

      Volume: 8 Pages: 1002558-1002558

    • Related Report
      2011 Final Research Report
  • [Journal Article] Nucleofection-based gene targeting in human pie-B cells2012

    • Author(s)
      Kurosawa A, Saito S, Mort M, Adachi N.
    • Journal Title

      Gene

      Volume: 492 Pages: 305-308

    • Related Report
      2011 Final Research Report
  • [Journal Article] Impaired cell adhesion, apoptosis, and signaling in WASP genedisrupted Nalm-6 pie-B cells and recovery of cell adhesion using a transducible form of WASp2012

    • Author(s)
      Sate R, Iiizumi S, Kim ES, Honda F, Lee SK, Adachi N, Koyama H, Mizutani S, Morie T.
    • Journal Title

      Int J Hematol

      Volume: 95 Pages: 299-310

    • Related Report
      2011 Final Research Report
  • [Journal Article] The helicase domain and C-terminus of human RecQL4 facilitate replication elongation on DNA templates damaged by ionizing radiation2012

    • Author(s)
      Adachi N., et al
    • Journal Title

      Carcinogenesis

      Volume: (印刷中)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nucleofection-based gene targeting in human pre-B cells2012

    • Author(s)
      Kurosawa A, Adachi N
    • Journal Title

      Gene

      Volume: 492 Pages: 305-308

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] LEDGF/p75-independent HIV-1 replication demonstrates a role for HRP-2 and remains sensitive to inhibition by LEDGINs2012

    • Author(s)
      Adachi N., et al
    • Journal Title

      PLoS Pathogens

      Volume: 8

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Highefficiency gene tar geting in a human pie-B cell line : towards the human gene knockout project2010

    • Author(s)
      Adachi N.
    • Journal Title

      Genome Biol

      Volume: 11 Pages: 1-1

    • Related Report
      2011 Final Research Report
  • [Journal Article] Functions and regulation of Artemis : A goddess in the maintenance of genome integrity2010

    • Author(s)
      Kurosawa A, Adachi N.
    • Journal Title

      J Radiat Res

      Volume: 51 Pages: 503-509

    • NAID

      10026644156

    • Related Report
      2011 Final Research Report
  • [Journal Article] OGFODl, a member of the 2oxoglutarate and iron dependent dioxygenase family, functions in ischemic signaling2010

    • Author(s)
      Saito K, Adachi N, Koyama H, Matsushita M.
    • Journal Title

      FEBS Left

      Volume: 584 Pages: 3340-3347

    • Related Report
      2011 Final Research Report
  • [Journal Article] Chromosomal manipulation by sitespecific recombinases and fluore scent protein-based vectors2010

    • Author(s)
      Uemura M, Niwa Y, Kakazu N, Adachi N, Kinoshita K.
    • Journal Title

      PLoS ONE

      Volume: 5 Pages: 9846-9846

    • Related Report
      2011 Final Research Report
  • [Journal Article] FENl functions in long patch base excision repair under conditions of oxidative stress in vertebrate cells2010

    • Author(s)
      Asagoshi K, Tana K, Chastain PD II, Adachi N, Sonoda E, Kikuchi K, Koyama H, NagataK, Kaufman DG, Takeda S, Wilson SH, Watanabe M, Swenberg JA, Nakamura J.
    • Journal Title

      Mol Cancer Res

      Volume: 8 Pages: 204-215

    • Related Report
      2011 Final Research Report
  • [Journal Article] Functions and regulation of Artemis : A goddess in the maintenance of genome integrity.2010

    • Author(s)
      Kurosawa A, Adachi N.
    • Journal Title

      J.Radiat.Res.

      Volume: 51 Pages: 503-509

    • NAID

      10026644156

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] OGFOD1, a member of the 2-oxoglutarate and iron dependent dioxygenase family, functions in ischemic signaling.2010

    • Author(s)
      Saito K., et al.
    • Journal Title

      FEBS Lett.

      Volume: 584 Pages: 3340-3347

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chromosomal manipulation by site-specific recombinases and fluorescent protein-based vectors2010

    • Author(s)
      Adachi, N., et al.
    • Journal Title

      PLoS ONE 5

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] FEN1 functions in long patch base excision repair under conditions of oxidative stress in vertebrate cells2010

    • Author(s)
      Adachi, N., et al.
    • Journal Title

      Mol.Cancer Res. 8

      Pages: 204-215

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functions and regulation of Artemis : A goddess in the maintenance of genome integrity2010

    • Author(s)
      Kurosawa, A., Adachi, N.
    • Journal Title

      J.Radiat.Res. 51(印刷中)

    • NAID

      10026644156

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The iron chelator Dp44mT causes DNA damage and selective inhibition of topoisomer ase-Ilalpha in breast cancer cells2009

    • Author(s)
      Rae VA, Klein SR, Agama KK, Toyoda E, Adachi N, Pommier YG, Shacter EB.
    • Journal Title

      Cancer Res

      Volume: 69 Pages: 948-957

    • Related Report
      2011 Final Research Report
  • [Journal Article] Loss of Bloom syndrome protein destabilizes human gene cluster architecture2009

    • Author(s)
      Killen MW, Stults DM, Adachi N, Hanakahi L, Pierce AJ.
    • Journal Title

      Hum Mol Genet

      Volume: 18 Pages: 3417-3428

    • Related Report
      2011 Final Research Report
  • [Journal Article] Genetic evidence that the nonhomologous endjoining repair pathway is involved in LINE retro transposition2009

    • Author(s)
      Suzuki J, Yamaguchi K, Kajikawa M, Ichiyanagi K, Adachi N, Koyama H, Takeda S, Okada N.
    • Journal Title

      PLoS Genetics

      Volume: 5 Pages: 1000461-1000461

    • Related Report
      2011 Final Research Report
  • [Journal Article] Topoisomerase Ilalpha inhib ition following DNA transfection greatly enhances random integration in a human pie-B lymphocyte cell line2009

    • Author(s)
      Toyoda E, Kurosawa A, Kamekawa H, Adachi N.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 382 Pages: 492-496

    • Related Report
      2011 Final Research Report
  • [Journal Article] Heterozygous disruption of the DNA topoisomerase I gene confers cellular resistance to camptothecin in human cells2009

    • Author(s)
      Toyoda E, Kurosawa A, Fu j i i M, Adachi N.
    • Journal Title

      Biol Pharm Bull

      Volume: 32 Pages: 724-727

    • NAID

      110007160669

    • Related Report
      2011 Final Research Report
  • [Journal Article] Molecular mechanism of protein assembly on DNA double strand breaks in the nonhomologous endjoining pathway2009

    • Author(s)
      Yano K, Morotomi-Yano K, Adachi N, Akiyama H.
    • Journal Title

      J Radiat Res

      Volume: 50 Pages: 97-108

    • NAID

      10025911271

    • Related Report
      2011 Final Research Report
  • [Journal Article] Model for MLL translocations in therapyrelated leukemia involving topoisomerase Ilbeta mediated DNA strand breaks and gene proximity

    • Author(s)
      DCowell IG, Sondka Z, Smith K, Lee KC, Manville CM, SidorczukLesthuruge M, Rance HA, Padget K, Jackson GH, Adachi N, Austin CA.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: (in press)

    • Related Report
      2011 Final Research Report
  • [Journal Article] The helicase domain and Cterminus of human RecQL4 facilitate replication elongation on DNA templates damaged by ionizing radiation

    • Author(s)
      Kohzaki M, Chiourea M, Versini G, Adachi N, Takeda S, Gages S, Halazonetis TD.
    • Journal Title

      Carcinogenesis

      Volume: (in press)

    • Related Report
      2011 Final Research Report
  • [Journal Article] Artemis Cterminal region facilitates efficient V(D) J recombination through its interaction with both DNA Ligase IV and DNA-PKcs

    • Author(s)
      Malu S, De loannes P, Kozlov M, Greene M, Francis, D, Hanna M, Pena, J, Escalante CR, Kurosawa A, ErdjumentBromage H, Tempst P, Adachi N, Vezzoni P, Villa A, Aggarwal AK, Certes P.
    • Journal Title

      J Exp Med

      Volume: (in press)

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://dnar.sci.yokohama-cu.ac.jp/

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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