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Study on acidic environment formation by V-ATPase using single molecule observation

Research Project

Project/Area Number 21590068
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionIwate Medical University

Principal Investigator

NAKANISHI Mayumi  岩手医科大学, 薬学部, 准教授 (20270506)

Co-Investigator(Kenkyū-buntansha) 二井 將光  岩手医科大学, 薬学部, 教授 (50012646)
後藤 奈緒美  岩手医科大学, 薬学部, 助教 (80403971)
Co-Investigator(Renkei-kenkyūsha) FUTAI Masamitsu  岩手医科大学, 薬学部, 教授 (50012646)
GOTO Naomi  岩手医科大学, 薬学部, 助教 (80403971)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsプロトンポンプ / 一分子観察 / 破骨細胞 / 酸性環境 / 骨代謝 / 分泌
Research Abstract

We have found that osteoclast-specific V-ATPase(vacuole-type proton-pumping ATPase) is composed of a3 and d2 isoforms. V-ATPase with a3 isoform is localized on lysosomal membrane, and is essential for traffic of lysosomes to plasmamembrane. Secretion of lysosomal enzymes and acidification outside osteoclasts are important for bone resorption. On the other hand, we have established experimental systems to observe subunit rotation of single molecule V-ATPase and to examine assembly of the catalytic domain and the proton pathway. These systems are essential to elucidate molecular mechanism that creates various acidic environments.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (25 results)

All 2012 2010 2009 Other

All Journal Article (12 results) (of which Peer Reviewed: 5 results) Presentation (11 results) Book (2 results)

  • [Journal Article] Proton pumping ATPase Rotational Catalysis ; from F-ATPase toward Mammalian V-ATPase.2012

    • Author(s)
      M. Futai, M. Nakanishi-Matsui, H. Okamoto, M. Sekiya, and R. K. Nakamoto
    • Journal Title

      Biochim, Biophys. Acta, 17^<th> European Bioenergetics Conference

      Volume: (In press)

    • Related Report
      2011 Final Research Report
  • [Journal Article] Proton pumping ATPase Rotational Catalysis ; from F-ATPase toward Mammalian V-ATPase. In press2012

    • Author(s)
      Masamitsu Futai, Mayumi Nakanishi-Matsui, et al
    • Journal Title

      Biochim, Biophys.Acta

      Volume: (in press) Issue: 10 Pages: 1711-1721

    • DOI

      10.1016/j.bbabio.2012.03.015

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of theβsubunit hinge domain in ATP synthase F_1 sector : hydrophobic network formed by introducedβPhe174 inhibits subunit rotation2010

    • Author(s)
      M. Nakanishi-Matsui, S. Kashiwagi, M. Kojima, T. Nonaka, and M. Futai
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 395 Pages: 173-177

    • Related Report
      2011 Final Research Report
  • [Journal Article] Single molecule behavior of inhibited and active states of Escherichia coli ATP synthase F_1 rotation2010

    • Author(s)
      M. Sekiya, H. Hosokawa, M. Nakanishi-Matsui, M. K. Al-Shawi, R. K. Nakamoto, and M. Futai
    • Journal Title

      J. Biol. Chem.

      Volume: 285 Pages: 42058-42067

    • Related Report
      2011 Final Research Report
  • [Journal Article] The mechanism of rotating proton-pumping ATPases2010

    • Author(s)
      M. Nakanishi-Matsui, M. Sekiya, R. K. Nakamoto, and M. Futai
    • Journal Title

      Biochim, Biophys. Acta

      Volume: 1797 Pages: 1343-1352

    • Related Report
      2011 Final Research Report
  • [Journal Article] プロトンポンプATPaseの回転触媒機構2010

    • Author(s)
      中西(松井)真弓、關谷瑞樹
    • Journal Title

      日本応用酵素協会誌

      Volume: No.45 Pages: 11-18

    • Related Report
      2011 Final Research Report
  • [Journal Article] Roles of the β Subunit Hinge Domain in ATP Synthase F_1 Sector : Hydrophobic Network Formed by Introduced βPhe174 Inhibits Subunit Rotation2010

    • Author(s)
      Mayumi Nakanishi-Matsui, Masamitsu Futai, et al.
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 395 Pages: 173-177

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The mechanism of rotating proton pumping ATPases.2010

    • Author(s)
      Mayumi Nakanishi-Matsui, Masamitsu Futai, et al.
    • Journal Title

      Biochim.Biophys.Acta

      Volume: 1797 Pages: 1343-1352

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single molecule behavior of inhibited and active states of Escherichia coli ATP synthase F_1 rotation.2010

    • Author(s)
      Mizuki Sekiya, Mayumi Nakanishi-Matsui, et al.
    • Journal Title

      J.Biol.Chem.

      Volume: 285 Pages: 42058-42067

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] プロトンポンプATPaseの回転触媒機構2010

    • Author(s)
      中西真弓、関谷瑞樹
    • Journal Title

      日本応用酵素協会誌

      Volume: 45 Pages: 11-18

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Temperature dependence of single molecule rotation of the Escherichia coli ATP synthase F_1 sector reveals the importance ofγ□βsubunit interactions in the catalytic dwell2009

    • Author(s)
      M. Sekiya, R. K. Nakamoto, M. K. Al-Shawi, M. Nakanishi-Matsui, and M. Futai
    • Journal Title

      J. Biol. Chem.

      Volume: 284 Pages: 22401-22410

    • Related Report
      2011 Final Research Report
  • [Journal Article] The mechanism of rotating proton pumping ATPases.

    • Author(s)
      Mayumi Nakanishi-Matsui, Masamitsu Futai, et al.
    • Journal Title

      Biochim.Biophys.Acta (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 破骨細胞が有する新規なイソフォーム構成のV-ATPase2012

    • Author(s)
      松元奈緒美, 中西(松井)真弓, 二井將光
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Different Localization of Specific V-ATPase between Osteoclast and LPS-induced Multinuclear Cells2010

    • Author(s)
      Mayumi Nakanishi-Matsui, Masamitsu Futai, et al.
    • Organizer
      FASEB, Summer Research Conferences Transport ATPases : From Molecules to Maladies
    • Place of Presentation
      米国コロラド州スノーマス
    • Year and Date
      2010-06-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Thermodynamics of single molecule rotation of Escherschia coli F-ATPase2010

    • Author(s)
      Mizuki Sekiya, Mayumi Nakanishi-Matsui, et al.
    • Organizer
      FASEB, Summer Research Conferences Transport ATPases : From Molecules to Maladies
    • Place of Presentation
      米国コロラド州スノーマス
    • Year and Date
      2010-06-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 破骨細胞に発現するV-ATPaseの機能2010

    • Author(s)
      松元奈緒美, 中西(松井)真弓, 二井將光
    • Organizer
      未来医療開発プロジェクトキックオフミーティング
    • Place of Presentation
      盛岡
    • Related Report
      2011 Final Research Report
  • [Presentation] プロトンポンプATPaseの酸性環境形成機構の解明2010

    • Author(s)
      中西(松井)真弓
    • Organizer
      第36回応用酵素協会研究発表会
    • Place of Presentation
      大阪
    • Related Report
      2011 Final Research Report
  • [Presentation] Different localization of specific V-ATPase between osteoclast and LPS-induced multinuclear cells2010

    • Author(s)
      中西(松井)真弓、矢野志緒、松元奈緒美、二井將光
    • Organizer
      FASEB Summer research conferences
    • Place of Presentation
      Colorado, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] 破骨細胞におけるV-ATPase a3 サブユニット局在オルガネラの解析2009

    • Author(s)
      松元奈緒美、平郁子、中西(松井)真弓、二井將光, 他
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] Mechanism of proton transport ATPases2009

    • Author(s)
      Mayumi Nakanishi-Matsui, Masamitsu Futai, et al.
    • Organizer
      Iwate International Symposium on Biochemical Approaches to Drug Discovery
    • Place of Presentation
      岩手医科大学矢巾キャンパス
    • Year and Date
      2009-05-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] 破骨細胞におけるV-ATPase a3サブユニット局在オルガネラの解析2009

    • Author(s)
      松元奈緒美、平郁子、中西(松井)真弓、二井將光
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of organelles with V-ATPase a3 subunit in osteoclasts2009

    • Author(s)
      松元奈緒美、中西(松井)真弓、平郁子、二井將光
    • Organizer
      JBS Biofrontier Symposium on Biochemistry of pH Homeostasis and Proton Circuit
    • Place of Presentation
      岩手医科大学矢巾キャンパス
    • Related Report
      2011 Final Research Report
  • [Presentation] Subunit rotation of E. coli F_1-ATPase : Effects of mutations in theβsubunit hinge domain2009

    • Author(s)
      中西(松井)真弓, 柏木幸子, 小島正樹, 野中孝昌, 二井將光
    • Organizer
      JBS Biofrontier Symposium on Biochemistry of pH Homeostasis and Proton Circuit
    • Place of Presentation
      岩手医科大学矢巾キャンパス
    • Related Report
      2011 Final Research Report
  • [Book] Roles and Regulatory Mechanism of Proton Pumping V-ATPase in Spermatozoa and Epididymis Physiology. Testis : Anatomy, Physiology and Pathology.2012

    • Author(s)
      M. Nakanishi-Matsui, Sylvie Breton, Haruko Okamoto, and Masamitsu Futai
    • Publisher
      Nova Science Publishers(In press)
    • Related Report
      2011 Final Research Report
  • [Book] Testis : Anatomy, Physiology and Pathology. Roles and Regulatory Mechanism of Proton Pumping V-ATPase in Spermatozoa and Epididymis Physiology. In press2012

    • Author(s)
      Mayumi Nakanishi-Matsui, Masamitsu Futai, et al
    • Publisher
      Nova Science Publishers(in press)
    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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