Study of spatiotemporal expression of D-amino acid oxidases and D-amino acid contents in Caenorhabditis elegans
Project/Area Number |
21590071
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biological pharmacy
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Research Institution | Kitasato University |
Principal Investigator |
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 生化学 / D-アスパラギン酸 / D-アミノ酸 / D-アスパラギン酸酸化酵素 / D-アミノ酸酸化酵素 / 欠損変異株 / 線虫 / D-アスパーギン酸 |
Research Abstract |
Caenorhabditis elegans, a model organism has four genes which encode the enzymes degrading D-amino acids oxidatively. One of them encodes D-amino acid oxidase which degrades neutral and basic amino acids, while the other three are D-aspartate oxidases 1-3 degrading acidic D-amino acids. In this work, I isolated and prepared the mutants defective in each gene, and compared them with the wild type strain. First I determined by HPLC the contents of D-amino acids in the body of wild type and the mutants. Then I examined the phenotypes and behaviors of the mutants and compared with those of the wild type. Based on these results I discussed the physiological roles of D-amino acids.
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Report
(4 results)
Research Products
(72 results)
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[Presentation] Molecular characterization and tissue localization of nematode D-amino acid degradative enzymes2009
Author(s)
Saitoh, Y., Katane, M., Seida, Y., Furuchi, T., Kobuna, K., Sakamoto, T., Inoue, T., Arai, H., Nakagawa, Y., Homma, H.
Organizer
The first international conference of D-amino acidresearch
Place of Presentation
Awaji, Hyogo
Year and Date
2009-07-03
Related Report
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