Studies on the crosstalk between biosynthesis and degradation of prostaglandin E2

• Project/Area Number: 21590074
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Showa University
• Principal Investigator: NAKATANI Yoshihito 昭和大学, 薬学部, 講師 (80266163)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: プロスタグランジンE2 / cPGES/p23 / 15-PGDH / PGE2 / cPGES / PGT / 23 / トランスジェニックマウス

Research Abstract

We revealed that cytosolic prostaglandin E synthase(cPGES/p23) which isomerizes PGH2 to PGE2, promotes the expression of 15-hydroxyPG dehydrogenase(15-PGDH), which catalyzes the inactivating conversion of the PGE2 15-OH to a 15-keto group. This result suggests that the PGE2-inactivating pathway is controlled by the PGE2 biosynthetic enzyme, cPGES/p23.

Research Products

• [Journal Article] Involvement of the constitutive prostaglandin E synthase cPGES/p23 in expression of an initial prostaglandin E2 inactivating enzyme, 15-PGDH (2011)
  • Author(s): Nakatani Y, Hokonohara Y, Tajima Y, Kudo I, Hara S
  • Journal Title: Prostaglandins Other Lipid Mediat Volume: 94 Pages: 112-117
• [Journal Article] Involvement of the constitutive prostaglandin E synthase cPGES/p23 in expression of an initial prostaglandin E2 inactivating enzyme, 15-PGDH (2011)
  • Author(s): Nakatani Y, Hokonohara Y, Tajima Y, Kudo I, Hara S
  • Journal Title: Prostaglandins other Lipid Mediat Volume: 94 Pages: 112-117
  • Peer Reviewed
• [Journal Article] Mitochondrial dysfunction and reduced prostaglandin synthesis in skeletal muscle of Group VIB Ca2+-independent phospholipase A2gamma-deficient mice (2010)
  • Author(s): Yoda E, Hachisu K, Taketomi Y, Yoshida K, Nakamura M, Ikeda K, Taguchi R, Nakatani Y, Kuwata H, Murakami M, Kudo I, Hara S
  • Journal Title: J Lipid Res Volume: 51 Pages: 3003-3015
• [Journal Article] Mitochondrial dysfunction and reduced prostaglandin synthesis in skeletal muscle of Group VIB Ca2+-independent phospholipase A2 γ-deficient mice. (2010)
  • Author(s): Yoda E, Hachisu K, Taketomi Y, Yoshida K, Nakamura H, Ikeda K, Taguchi R, Nakatani Y, et al.
  • Journal Title: J.Lipid Res. Volume: 51 Pages: 3003-3015
  • Peer Reviewed
• [Journal Article] Microsomal prostaglandin E synthase-1 in both cancer cells and hosts contributes to tumour growth, invasion and metastasis. (2010)
  • Author(s): D.Kamei, M.Murakami, Y.Sasaki, Y.Nakatani, et al.
  • Journal Title: Biochem.J. 425 Pages: 361-371
  • Peer Reviewed
• [Journal Article] Microsomal prostaglandin E synthase-1 in both cancer cells and hosts contributes to tumour growth, invasion and metastasis (2009)
  • Author(s): Kamei D, Murakami M, Sasaki Y, Nakatani Y, Majima M, Ishikawa Y, Ishii T, Uematsu S, Akira S, Hara S, Kudo I
  • Journal Title: Biochem J Volume: 425 Pages: 361-371
• [Presentation] cPGES/p23の細胞内機能の解析 (2012)
  • Author(s): 中谷良人、高村茜、中坪智美、原俊太郎
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-29
• [Presentation] cPGES/p23の細胞内機能の解析 (2012)
  • Author(s): 中谷良人, 岡村恵理子、無藤大地、加倉井優、大本俊介、原俊太郎
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌市
  • Year and Date: 2012-03-29
• [Presentation] 細胞質型プロスタグランジンE合成酵素cPGES/p23の解析 (2011)
  • Author(s): 中谷良人, 岡村恵理子、無藤大地、加倉井優、大本俊介、原俊太郎
  • Organizer: フォーラム2011衛生薬学・環境トキシコロジー
  • Place of Presentation: 金沢市
  • Year and Date: 2011-10-28
• [Presentation] 細胞質型プロスタグランジンE合成酵素cPGES/p23の解析 (2011)
  • Author(s): 中谷良人、岡村恵理子、無藤大地、加倉井優、大本俊介、原俊太郎
  • Organizer: フォーラム2011衛生薬学・環境トキシコロジー
  • Place of Presentation: 金沢
  • Year and Date: 2011-10-27
• [Presentation] ケラチノサイトの細胞生存に対するプロスタグランジンE2の影響 (2011)
  • Author(s): 岡村浩志、高村茜、中谷良人、原俊太郎
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-30
• [Presentation] ケラチノサイトを用いたプロスタグランジンE2の細胞死への関与の解析 (2010)
  • Author(s): 岡村浩志、高村茜、中谷良人、原俊太郎
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] ケラチノサイトを用いたプロスタグランジンE2の細胞死への関与の解析 (2010)
  • Author(s): 岡村浩志、中谷良人、原俊太郎
  • Organizer: フォーラム2010衛生薬学・環境トキシコロジー
  • Year and Date: 2010-09-09
• [Presentation] 細胞内ホスホリパーゼA2による走化性因子産生の制御 (2010)
  • Author(s): 桑田浩、原田和佳、譲原千尋、滝雄貴、依田恵美子、中谷良人、原俊太郎
  • Organizer: 第52回日本脂質生化学会
  • Place of Presentation: 伊香保
  • Year and Date: 2010-06-14
• [Presentation] Involvement of cytosolic prostaglandin E synthase(cPGES/p23) in PGE2 signal termination (2010)
  • Author(s): Yoshihito Nakatani, Masahiro Okada, Yutaka Hokonohara, Ichiro Kudo, Shuntaro Hara
  • Organizer: 2010 Keystone Symposia Bioactive Lipids : Biochemistry and Diseases
  • Place of Presentation: 京都
  • Year and Date: 2010-06-09
• [Presentation] Involvement of cytosolic prostaglandin E synthase (cPGES/p23) in PGE2 signal termination (2010)
  • Author(s): Nakatani Y
  • Organizer: 2010 Keystone Symposia Bioactive Lipids : Biochemistry and Diseases
  • Place of Presentation: 京都
  • Year and Date: 2010-06-08
• [Presentation] Biosynthesis of prostaglandin E2 in embryonic fibroblasts (2010)
  • Author(s): Nakatani Y
  • Organizer: 3rd Pan Pacific Symposium on Stem Cells Research
  • Place of Presentation: 台北(台湾)
  • Year and Date: 2010-04-18
• [Presentation] Biosynthesis of prostaglandin E2 in embryonic fibroblasts (2010)
  • Author(s): Yoshihito Nakatani, Ichiro Kudo, Shuntaro Hara
  • Organizer: 3rd Pan Pacific Symposium on Stem Cells Research
  • Place of Presentation: 台北
  • Year and Date: 2010-04-17
• [Remarks]
  • URL: http://www.showa-u.ac.jp/sch/pharm/major/hchem/edu_rsch/
• [Remarks]
  • URL: http://www.showa-u.ac.jp/sch/pharm/major/hchem/rsch_achievement/