Physiological roles of a novel bioactive lipid lysophosphatidylinositol

• Project/Area Number: 21590076
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Teikyo University
• Principal Investigator: SUGIURA Takayuki 帝京大学, 薬学部, 教授 (40130009)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 脂質生化学 / カンナビノイド / Gタンパク質共役型受容体 / リゾリン脂質 / リゾホスファチジルイノシトール / 生理活性脂質 / 脂質メディエーター / ホスホリパーゼA / イノシトールリン脂質 / GPR55 / マリファナ

Research Abstract

GPR55 is a novel cannabinoid receptor. We found that its endogenous ligand is lysophosphatidylinositol(LPI). In this study, we obtained evidence that LPI plays some essential roles in the regulation of stress fiber formation. We next searched for the endogenous ligand for GPR35 which has 30% homology with GPR55.We found that 2-acyl lysophosphatidic acid(LPA) may act as an endogenous ligand for GPR35.

Research Products

• [Journal Article] 脂質メディエーターとしてのリゾホスファチジルイノシトール (2011)
  • Author(s): 杉浦隆之, 山下純, 岡沙織
  • Journal Title: 生化学 Volume: 83 Pages: 525-535
  • Peer Reviewed
• [Journal Article] 脂質メディェーターとしてのリゾホスファチジルイノシトールとその受容体 (2011)
  • Author(s): 杉浦隆之、山下純、岡沙織
  • Journal Title: 生化学 Volume: 83 Pages: 525-535
  • Peer Reviewed
• [Journal Article] Lysophosphatidylinositol induces rapid phosphorylation of p38 mitogen-activated protein kinase and activating transcription factor 2 in HEK293cells expressing GPR55 and IM-9 lymphoblastoid cells (2010)
  • Author(s): Oka, S., Yamashita, A., Sugiua, T. et al.(他3人, 6番目)
  • Journal Title: J. Biochem Volume: 147 Pages: 671-678
  • Peer Reviewed
• [Journal Article] GPR35 is a novel lysophosphatidic acid receptor (2010)
  • Author(s): Oka, S., Yamashita, A., Sugiura, T., et al.(他2人, 5番目)
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 395 Pages: 232-237
  • Peer Reviewed
• [Journal Article] Generation of lysophosphatidylinositol by DDHD domain containing 1(DDHD1): Possible involvement of phospholipase D/phosphatidic acid in the activation of DDHD1 (2010)
  • Author(s): Yamashita, A., Oka, S., Sugiura, T., et al.(他3人, 6番目)
  • Journal Title: Biochem. Biophys. Acta Volume: 1801 Pages: 711-720
  • Peer Reviewed
• [Journal Article] Generation of lysophosphatidylinositol by DDHD domain containing 1 (DDHD1). Possible involvement of phospholipase D/phosphatidic acid in the activation of DDHD1 (2010)
  • Author(s): Yamashita, A., et al.
  • Journal Title: Biochim.Biophys.Acta Volume: 1801 Pages: 711-720
  • Peer Reviewed
• [Journal Article] GPR35 is a novel lysophosphatidic acid receptor. (2010)
  • Author(s): Oka, S., Sugiura, T., et al.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 395 Pages: 232-237
  • Peer Reviewed
• [Journal Article] Lysophosphatidylinositol induces rapid phospholylation of p38 mitogen-activated protein kinase and activating transcription factor 2 in HEK293 cells expressing GPR55 and IM-9 lymphoblastoid cells. (2010)
  • Author(s): Oka, S., et al.
  • Journal Title: J.Biochem. 65(In press)
  • Peer Reviewed
• [Journal Article] Subcellular localization and lysophospholipase/transacylation activities of human group IVC phospholipase A2(cPLA2γ) (2009)
  • Author(s): Yamashita, A., Waku, K., Sugiura, T., et al.(他5人, 8番目)
  • Journal Title: Biochem. Biophys. Acta Volume: 1791 Pages: 1011-1022
  • Peer Reviewed
• [Journal Article] Physiological roles of 2-arachidonoylglycerol, an endogenous cannabinoid receptor ligand (2009)
  • Author(s): Sugiura T
  • Journal Title: Biofactors Volume: 35 Pages: 88-97
  • Peer Reviewed
• [Journal Article] 内在性カンナビノイド受容体リガンドとメタボリックシンドローム (2009)
  • Author(s): 杉浦隆之
  • Journal Title: The Lipid Volume: 20 Pages: 21-27
• [Journal Article] 油脂・脂質の基礎と応用-栄養・健康から工業まで(日本油学会編)日本油化学協会 (2009)
  • Author(s): 杉浦隆之
  • Journal Title: 生理活性脂質
• [Journal Article] Synthesis and biological activities of 14-epi-MART-10 and 14-epi-MART-11 : implications for cancer and osteoporosis treatment (2009)
  • Author(s): Kittaka, A, Sugiura, T., Chen, TC., et al.(他13人, 15番目)
  • Journal Title: Anticancer Res Volume: 29 Pages: 3563-3569
  • Peer Reviewed
• [Journal Article] Synthesis of a 1 a-C-methyl analogue of 25-hydroxyvitamin D_3 : interaction with a mutant vitamin D receptor Arg274Leu (2009)
  • Author(s): Honzawa, H., Sugiura, T., Kittaka, A.(他5人, 4番目)
  • Journal Title: Tetrahedron Volume: 65 Pages: 7135-7145
  • Peer Reviewed
• [Journal Article] 2-Arachinonyl-sn-glycero-3-phosphoinositol : a possible natural ligand for GPR55. (2009)
  • Author(s): Oka, S., et al.
  • Journal Title: J.Biochem. 145 Pages: 13-20
  • Peer Reviewed
• [Journal Article] Subcellular localization and lysophospholipase/transacylation reactions of humangroup IVC phospholipase A2 (cPLA2g). (2009)
  • Author(s): Yamashita, A., et al.
  • Journal Title: Biochim. Biophys. Acta 1791 Pages: 1011-1022
  • Peer Reviewed
• [Presentation] Lysophosphatidylinositol and Related Lipid Mediators (2011)
  • Author(s): Takayuki Sugiura
  • Organizer: FASEB Summer Research Conference
  • Place of Presentation: イタリア・ルッカ
  • Year and Date: 2011-08-15
• [Presentation] Lisophosphatidyl-inositol and related madiators (2011)
  • Author(s): 杉浦隆之, 他
  • Organizer: 2011 FASEB Summer Research Conference
  • Place of Presentation: イタリア
• [Presentation] GPR55 and GPR35, putativeΔ^9-tetrahydrocannabinol receptors, and their endogenous ligand (2010)
  • Author(s): 杉浦隆之, 他
  • Organizer: 第26回国際カンナビノイド学会(ICRS2010)
  • Place of Presentation: Scandic star(Sweden)
  • Year and Date: 2010-07-25
• [Presentation] GPR55 and GPR35, putative Δ^9-tetrahydrocannabinol receptors, and their endogenous ligands (2010)
  • Author(s): Sugiura T.
  • Organizer: 国際カンナビノイド会議(ICRS)
  • Place of Presentation: スウエーデン、ルンド
  • Year and Date: 2010-07-25
• [Presentation] Lysophosphatidylinositol induces rapid cytoskeltal rearrangements and morphological changesin HEK293 cells expressing GPR55 (2009)
  • Author(s): 杉浦隆之
  • Organizer: 国際カンナビノイド会議(ICRS)
  • Place of Presentation: ST. CHARLES,(ILLINOIS, USA)
  • Year and Date: 2009-07-08
• [Presentation] Lysophosphatidylinositol : an endogenous ligand for GPR55, a putative novel cannabinoid receptor (2009)
  • Author(s): 杉浦隆之
  • Organizer: 4th International Conference on Phospholipase A2 and Lipid Mediators(PLM2009)
  • Place of Presentation: 学術総合センター
  • Year and Date: 2009-05-27
• [Presentation] Lysophosphatidylinositol : an endogenous ligand for GPR55, a putative novel cannabinoid receptor (2009)
  • Author(s): 杉浦隆之
  • Organizer: 4th International Conference on Phospholipase A2 and Lipid Mediators, PLM2009
  • Place of Presentation: Tokyo
• [Presentation] Lysophosphatidylinositol induces rapid cytoskeltal rearrangements and morphological changes in HEK293 cells expressing GPR55 (2009)
  • Author(s): 杉浦隆之
  • Organizer: 19th International Cannabinoid Research Society Meeting
  • Place of Presentation: Chicago
• [Remarks]
  • URL: http://www.pharm.teikyo-u.ac.jp/lab/eisei/index.html
• [Remarks]
  • URL: http://www.pharm.teikyo-u.ac.jp/lab/eisei/index.html