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The mechanism of amyloid fibril formation induced by D-amino acids

Research Project

Project/Area Number 21590080
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionMusashino University

Principal Investigator

TATE Naoko  武蔵野大学, 薬学研究所, 教授 (00201955)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsアルツハイマー病 / アミロイドβペプチド / 線維化 / 凝集 / D-アミノ酸 / アミロイドβペプチド(Aβ) / Aβフラグメント / アミロイドβペブチド / 老人斑 / 老年性脳疾患 / 構造 / 物性 / 神経細胞毒性
Research Abstract

Amyloid β proteins(Aβ) extracted from the amyloid cores of neutritic plaques are considerably isomerized at their Asp residues. To asseses the impact of D-Asp on Aβ conformation. and on initiation of amyloid fibril formation, I used wild type Aβ and D-Asp-containing Aβ which D-Asp. was substituted for L-Asp at residues 1, 7, 23. Amyloid fibril formation was enhanced by D-Asp23. Moreover, I demonstrated that D-Asp containing Aβ showed no effect on fibril formation of wild-type Aβ. Lastly, I showed that A. fragment 1-23 promoted formation of fibrils and aggregates of wild-type Aβ.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010 2009

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (16 results)

  • [Journal Article] D-Ser containing hymanin shows promotion of fibril formation2012

    • Author(s)
      Hayashi, K., Sasabe, J., Chiba, T., Aiso, S. and Utsunomiya-Tate, N
    • Journal Title

      Amino Acids

      Volume: 42 Pages: 2293-2297

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Controlled structure and properties of silicate nanoparticle networks for incorporation of biosystem components2012

    • Author(s)
      Hayashi, K., Sasabe, J., Chiba, T., Aiso, S., Utsunomiya-Tate, N.
    • Journal Title

      Amino Acids

      Volume: 22 Issue: 20 Pages: 2293-2297

    • DOI

      10.1088/0957-4484/22/20/205702

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Controlled structure and properties of silicate nanoparticle networks for incorporation of biosystem components2011

    • Author(s)
      Sakai-Kato, K., Hasegawa, T., Takaoka, A., Kato, M, Toyo'oka, T., Utsunomiya-Tate, N. and Kawanishi, T
    • Journal Title

      Nanotechnology

      Volume: 22 Pages: 1-8

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Collagen racemization and deposition in the lungs of aged rats2010

    • Author(s)
      Kasai, A., Yamashita, N. and Utsunomiya-Tate, N
    • Journal Title

      Biochemistry Insights

      Volume: 3 Pages: 25-33

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stability of folding structure of Zic zinc finger proteins2009

    • Author(s)
      Sakai-Kato, K., Umezawa, Y., Mikoshiba, K., Aruga, J. and Utsunomiya-Tate, N
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 384 Pages: 362-365

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stability of folding structure of Zic zinc finger proteins2009

    • Author(s)
      Sakai-Kato, K.
    • Journal Title

      Biochem. Biophys. Res.Commun. 384

      Pages: 362-365

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] D-アスパラギン酸含有アミロイドβペプチドの線維・凝集体形成および神経細胞毒性に関する解析2011

    • Author(s)
      林周宏、楯直子
    • Organizer
      第84回日本生化学会
    • Place of Presentation
      京都国際会館(京都)
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] D-アスパラギン酸含有アミロイドβペプチドの線維・凝集体形成および神経細胞毒性に関する解析2011

    • Author(s)
      林周宏、大平麻里、楯直子
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] 正常型HumaninおよびD-Ser含有Humaninの構造・物性に関する研究2011

    • Author(s)
      林周宏、楯直子
    • Organizer
      日本薬学会第131回年会
    • Place of Presentation
      ツインメッセ静岡(静岡)
    • Year and Date
      2011-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 正常型HumaninおよびD-Ser含有Humaninの構造・物性に関する研究2011

    • Author(s)
      林周宏
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      ツインメッセ静岡(静岡)
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Preferential domain orientation in HMGB2 protein revealed by NMR spectroscopy2010

    • Author(s)
      Uewaki, J., and Utsunomiya-Tate, N
    • Organizer
      PACIFICHEM2010
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2010-12-18
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] アミロイドβペプチドのアスパラギン酸残基異性化置換による線維化・凝集機構における構造機能相関解析2010

    • Author(s)
      楯直子
    • Organizer
      日本生化学会第83回大会
    • Place of Presentation
      神戸ポートアイランド(兵庫)
    • Year and Date
      2010-12-07
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 神経保護作用タンパク質Humanin及びその誘導体の生物構造学的解析2010

    • Author(s)
      林周宏
    • Organizer
      日本生化学会第83回大会
    • Place of Presentation
      神戸ポートアイランド(兵庫)
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 脳特異的RasGEF,very-KIND-MAP2結合における構造機能相関2010

    • Author(s)
      林周宏
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] D-Asp含有改変型アミロイドβペプチドの神経細胞毒性に関する研究2010

    • Author(s)
      楯直子
    • Organizer
      日本薬学会第130回年会
    • Place of Presentation
      岡山大学(岡山県)
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] 加齢に伴うアルツハイマー病関連タンパク質中のD-アミノ酸量の変化に関する解析2010

    • Author(s)
      河西亜希子
    • Organizer
      日本薬学会第130回年会
    • Place of Presentation
      岡山大学(岡山県)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Analysis of structure stability of HMGB22009

    • Author(s)
      森内寛、楯直子
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アクティ徳島(徳島)
    • Year and Date
      2009-10-30
    • Related Report
      2011 Final Research Report
  • [Presentation] D-amino acids are contained in the Altzheimer disease-related protein in the brain of SAMP82009

    • Author(s)
      河西亜希子
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アクティ徳島 (徳島県)
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Analysis of structure stability of HMGB22009

    • Author(s)
      森内寛
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アクティ徳島(徳島県)
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] アルツハイマー病関連タンパク質における加齢によるD-アミノ酸の産生と発症との関連性に関する解析2009

    • Author(s)
      河西亜希子
    • Organizer
      日本生化学会第82回大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2009-10-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] Influence of amino acid isomerization on proteins, which cause senescence-related diseases2009

    • Author(s)
      Moriuchi, H. and Utsunomiya-Tate, N
    • Organizer
      The 1^<st> international conference of D-amino acid research
    • Place of Presentation
      Awaji Yumebutai International Conference Center(Awaji, Hyougo, Japan)
    • Year and Date
      2009-07-02
    • Related Report
      2011 Final Research Report
  • [Presentation] Influence of amino acid isomerization on proteins, which cause senescence-related diseases.2009

    • Author(s)
      Moriuchi, H.
    • Organizer
      The 1^<st> international conference of D-amino acid research
    • Place of Presentation
      Awaji Yumebutai International Conference Center (Awaji, Hyougo, Japan)
    • Year and Date
      2009-07-02
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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