Specific regulation for NFAT based on diversity of the NFAT family

• Project/Area Number: 21590086
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: 財団法人東京都医学総合研究所 (2011); Tokyo Metropolitan Organization for Medical Research (2009-2010)
• Principal Investigator: KAMINUMA Osamu 財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主任研究員 (80342921)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 免疫学 / T細胞 / 免疫抑制薬 / 転写因子 / 分子間結合 / 発現調節

Research Abstract

By employing NFATc2/NFATc4 chimera molecules, it was clarified that NFATc4 down-regulate cytokine expression in T cells via its CRD domain. The quantitative CN/NFAT binding assay revealed that there is a novel CN binding region in the CRD domain. It was found that WWTR1 regulates specific expression of NFATc4 in aortic smooth muscle cells by means of a comparative microarray analysis.

Research Products

• [Journal Article] Zinc finger protein, multitype 1 suppresses human Th2 development via down-regulation of IL-4 (2011)
  • Author(s): Kitamura N, Mori A, Tatsumi H, Nemoto S, Miyoshi H, Miyatake S, Kitamura F, Hiroi T, Kaminuma O
  • Journal Title: Int Arch Allergy Immunol Volume: 155
  • Peer Reviewed
• [Journal Article] Comparative analysis of steroid sensitivity of Th cells in vitro and in vivo (2011)
  • Author(s): Abe A, Ohtomo T, Koyama S, Kitamura N, Kaminuma O, Mori A
  • Journal Title: Int Arch Allergy Immunol Volume: 155
  • Peer Reviewed
• [Journal Article] CD44 is critical for airway accumulation of antigen-specific Th2 cells, but not Th1 cells, induced by antigen challenge in mice (2011)
  • Author(s): Kato S, Kaminuma O, Hiroi T, Mori A, Ohtomo T, Maeda S, Shimizu H, Obase Y, Oka M
  • Journal Title: Eur J Immunol Volume: 41 Pages: 3198-3207
  • Peer Reviewed
• [Journal Article] Zinc finger protein, multitype 1 suppresses human Th2 development via down-regulation of IL-4 (2011)
  • Author(s): Kitamura N, Kaminuma O, et al
  • Journal Title: International Archives of Allegy and Immunology Volume: VOL.155 Issue: Suppl. 1 Pages: 53-56
  • DOI: 10.1159/000327292
  • Peer Reviewed
• [Journal Article] Comparative analysis of steroid sensitivity of Th cells in vitro and in vivo (2011)
  • Author(s): Abe A, Kaminuma O, et al
  • Journal Title: International Archives of Allegy and Immunology Volume: VOL.155 Issue: Suppl. 1 Pages: 110-116
  • DOI: 10.1159/000327494
  • Peer Reviewed
• [Journal Article] CD44 is critical for airway accumulation of antigen-specific Th2 cells, but not Th1 cells, induced by antigen challenge in mice (2011)
  • Author(s): Kato S, Kaminuma O, et al
  • Journal Title: European Journal of Immunology Volume: 41 Issue: 11 Pages: 3198-3207
  • DOI: 10.1002/eji.201141521
  • Peer Reviewed
• [Journal Article] Selective suppression of Th2 cytokines by C-terminal binding protein 2 in human T cells (2010)
  • Author(s): Kitamura F, Kitamura N, Mori A, Tatsumi H, Nemoto S, Miyoshi H, Miyatake S, Hiroi T, Kaminuma O
  • Journal Title: Int Arch Allergy Immunol Volume: 152
  • Peer Reviewed
• [Journal Article] Eosinophils are required for the induction of bronchial hyperresponsiveness in a Th-transfer model of Balb/c background (2010)
  • Author(s): Ohtomo T, Kaminuma O, Kitamura N, Suko M, Kobayashi N, Mori A
  • Journal Title: Int Arch Allergy Immunol Volume: 152
  • Peer Reviewed
• [Journal Article] Selective suppression of Th2 cytokines by C-terminal binding protein 2 in human T cells. (2010)
  • Author(s): Kitamura Fujiko, Kaminuma Osamu, et al.
  • Journal Title: International Archives of Allergy and Immunology Volume: VOL.152 Pages: 18-21
  • Peer Reviewed
• [Journal Article] Eosinophils are required for the induction of bronchial hyperresponsiveness in a Th-transfer model of Balb/c background. (2010)
  • Author(s): Ohtomo Takayuki, Kaminuma Osamu, et al.
  • Journal Title: International Archives of Allergy and Immunology Volume: VOL.152 Pages: 79-82
  • Peer Reviewed
• [Journal Article] NFATサブタイプ阻害薬開発の意義と可能性 (2009)
  • Author(s): 神沼修
  • Journal Title: 薬学研究の進歩 Volume: 25 Pages: 7-10
• [Journal Article] NEATサブタイプ阻害薬の意義と可能性 (2009)
  • Author(s): 神沼修
  • Journal Title: 薬学研究の進歩 25 Pages: 7-10
• [Presentation] Antigen-specific Th2 cells specifically utilize CD44 to infiltrate into antigen-challenged lung in mice (2011)
  • Author(s): Kaminuma O, Kato S, Mori A, Hiroi T
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 千葉
  • Year and Date: 2011-11-28
• [Presentation] T細胞のIL-17産生に対するNFATファミリーの機能的相違 (2011)
  • Author(s): 神沼修、北村紀子、北村ふじ子、巽英樹、根本荘一、宮武昌一郎、森晶夫、廣井隆
  • Organizer: アレルギー・好酸球研究会2011
  • Place of Presentation: 東京
  • Year and Date: 2011-06-18
• [Presentation] Comparative analysis of steroid sensitivity of Th cells in vitro and in vivo (2011)
  • Author(s): Mori A, Abe A, Koyama S, Kitamura N, Yamaguchi M, Tanimoto H, Sekiya K, Oshikata C, Mitsui C, Taniguchi M, Ohtomo M, Maeda Y, Hasegawa M, Akiyama K, Ohtomo T, Kaminuma O
  • Organizer: 30th Congress of the European Academy of Allergy and Clinical Immunology
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2011-06-13
• [Presentation] ZFPM1/CtBP1コンプレックスはGATA-3によるTh2分化を抑制する (2010)
  • Author(s): 神沼修,北村紀子,森晶夫,巽英樹,根本荘一,廣井隆親
  • Organizer: 第60回日本アレルギー学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-26
• [Presentation] ZFPM1/CtBP1コンプレックスはGATA-3によるTh2分化を抑制する (2010)
  • Author(s): 神沼修, ほか
  • Organizer: 第60回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京国際フォーラム・東京
  • Year and Date: 2010-11-26
• [Presentation] T細胞クローン移入喘息モデルによるステロイド感受性解析 (2010)
  • Author(s): 安部暁美、大友隆之、神山智、北村紀子、神沼修、森晶夫
  • Organizer: アレルギー・好酸球研究会2010
  • Place of Presentation: 東京
  • Year and Date: 2010-06-19
• [Presentation] T細胞クローン移入喘息モデルによるステロイド感受性解析 (2010)
  • Author(s): 安部暁美、神沼修, ほか
  • Organizer: アレルギー・好酸球研究会2010
  • Place of Presentation: 大手町サンケイプラザ・東京
  • Year and Date: 2010-06-19