| Project/Area Number |
21590109
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Kinki University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TSUJIUCHI Toshifumi 近畿大学, 理工学部, 教授 (10254492)
MORIYAMA Ryutaro 近畿大学, 理工学部, 講師 (30411573)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | リゾホスファチジン酸 / G蛋白質 / 神経軸索 / 分岐 / 神経ネットワーク / LPA3 / 神経分岐 / Rnd2 / リゾホスファジン酸 |
|---|
| Research Abstract |
Although neurite branching is crucial for neuronal network formation after birth, its underlying mechanisms remain unclear. Here, we demonstrate that lysophosphatidic acid(LPA) stimulates neurite branching through a novel signaling pathway. Treatment of neuronal cell lines with LPA resulted in neurite branch formation when LPA_3 receptor was introduced. The effects of LPA were blocked by inhibition of G_q signaling. Furthermore, expression of inhibitory mutants of the small GTPase Rnd2/Rho7 or an Rnd2 effector rapostlin abolished LPA_3-mediated neurite branching. The LPA_3 agonist 2(S)-OMPT or LPA also induced axonal branch formation in hippocampal neurons, which was blocked by G_q and Rnd2 pathway inhibition or LPA_3 knockdown. These findings suggest that the novel signaling pathway involving LPA_3, G_q, and Rnd2 may play an important role in neuronal network formation.
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