Studies for the mechanism of the mucosal immunity of mice and the application for desensitization of environmental allergens

• Project/Area Number: 21590148
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: National Institute of Health Sciences
• Principal Investigator: TESHIMA Reiko 国立医薬品食品衛生研究所, 代謝生化学部, 部長 (50132882)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Rhyosuke 国立医薬品食品衛生研究所, 代謝生化学学, 主任研究官 (50333357)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 粘膜免疫 / 免疫寛容 / 環境アレルゲン / 腸管上皮細胞 / エキソソーム

Research Abstract

We newly obtained following four results in our studies :(1) Increase of IgA production from peyer's patch IgA+B220-cells by addition of culture medium of small-intestinal epithelial cell line MoS13 cells stimulated with TLR1/2 ligand,(2) Decrease of IgE production by yeast glucomannan(YGM)-Fag e 1 conjugates treated mice,(3) Decrease of IL21-production and Tfh cell differentiation by re-stimulated peyer's patch cells obtained from OVA-oral-sensitized BALB/c mice,(4) Identification of T-cell epitopes of major buckwheat allergen Fag e 2.

Research Products

• [Journal Article] Effective induction of oral anaphyaxis to ovalbumin in mice sensitized by feeding of the antigen with aid of oil emulsion and salicylate (2012)
  • Author(s): Shindo T., Kanazawa Y., Saito Y., Kojima K., Ohsawa M., Teshima R
  • Journal Title: J. Toxicol. Sci Pages: 307-315
  • URL: http://dx.doi.org/10.2131/jts.37.307
  • Peer Reviewed
• [Journal Article] 経口感作の成立と消化管粘膜免疫機構 (2012)
  • Author(s): 手島玲子
  • Journal Title: アレルギー・免疫 Volume: 19(1) Pages: 40-44
• [Journal Article] 食物アレルゲンの消化と抗原性 (2012)
  • Author(s): 手島玲子
  • Journal Title: 臨床免疫・アレルギー科 Volume: 57(5) Pages: 556-561
  • NAID: 40019337466
• [Journal Article] Effective induction of oral anaphyaxis to ovalbumin in mice sensitized by feeding of the antigen with aid of oil emulsion and salicylate (2012)
  • Author(s): Shindo T., Kanazawa Y., Saito Y., Kojima K., Ohsawa M., Teshima R.
  • Journal Title: J.Toxicol.Sci. Volume: 37 Pages: 307-315
  • Peer Reviewed
• [Journal Article] ムノプロテオミクス手法を用いたソバIgE結合タンパク質の網羅的検出 (2011)
  • Author(s): 佐藤里絵、中村里香、手島玲子
  • Journal Title: 日本食品化学学会会誌 Volume: 18(2) Pages: 103-109
  • Peer Reviewed
• [Journal Article] 食品中のアレルゲンの予測 (2011)
  • Author(s): 手島玲子、中村亮介
  • Journal Title: 食品衛生学雑誌 Volume: 52 Issue: 12 Pages: 1-1
  • DOI: 10.1107/s1744309109043127
  • NAID: 130000671804
  • Peer Reviewed
• [Journal Article] イムノプロテオミクス手法を用いたソバIgE結合タンパク質の網羅的検出 (2011)
  • Author(s): 佐藤里絵、中村里香、手島玲子
  • Journal Title: 日本食品化学学会会誌 Volume: 18(2) Pages: 103-109
  • NAID: 110008897462
  • Peer Reviewed
• [Journal Article] A convenient and sensitive allergy test : IgE cross linking-inducedluciferase expression in cultured mast cells. (2010)
  • Author(s): Nakamura R., Uchida Y., Higuchi M., Nagokamura R., Teshima R.
  • Journal Title: Allergy Volume: 65 Issue: 10 Pages: 1266-1266
  • DOI: 10.1111/j.1398-9995.2010.02363.x
  • Peer Reviewed
• [Journal Article] Dietary carotenoids inhibit oral sensitization and the development of food allergy (2010)
  • Author(s): Sato Y, Akiyama H, Matsuoka H, Sakata K, Nakamura R, Totsuka M, Ebisawa M, Teshima R.
  • Journal Title: J. Agric. Food Chem. Volume: 58 Issue: 12 Pages: 7180-7180
  • DOI: 10.1021/jf100519x
  • Peer Reviewed
• [Journal Article] Identification of an IgE-binding epitope of a major buckwheat allergen, BWp16, by spot assay and mimotope screening (2010)
  • Author(s): Satoh R., Koyano S., Takagi K., Nakamura R., Teshima R.
  • Journal Title: Int. Arch. Allergy Immunol Volume: 153 Issue: 2 Pages: 133-133
  • DOI: 10.1159/000312630
  • Peer Reviewed
• [Journal Article] A convenient and sensitive allergy test : lgE crosslinking-induced luciferase expression in cultured mast cells (2010)
  • Author(s): Nakamura R., Uchida Y., Higuchi M., Nakamura R., Teshima R.
  • Journal Title: Allergy Volume: 65 Pages: 1266-1273
  • Peer Reviewed
• [Journal Article] Dietary carotenoids inhibit oral sensitization and the development of food allergy (2010)
  • Author(s): Sato Y, Akiyama H, Matsuoka H, Sakata K, Nakamura R, Totsuka M, Ebisawa M, Teshima R.
  • Journal Title: J.Agric.Food Chem. Volume: 58 Pages: 7180-7186
  • Peer Reviewed
• [Journal Article] Identification of an IgE-binding epitope of a major buckwheat allergen, BWp16, by spot assay and mimotope screening (2010)
  • Author(s): Satoh R., Koyano S., Takagi K., Nakamura R., Teshima R.
  • Journal Title: Int.Arch.Allergy Immunol 153 Pages: 133-140
  • Peer Reviewed
• [Journal Article] Modulation of immunoresponse in BALB/c mice by oral administration of Fag e 1-glucomannan conjugate (2009)
  • Author(s): Suzuki Y., Kassai M. Hirose T., Katayama K., Nakamura K., Akiyama H., Teshima R., Nakamura S.
  • Journal Title: J. Agric. Food Chem Volume: 57 Issue: 20 Pages: 9787-9787
  • DOI: 10.1021/jf902490t
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of a deletion mutant of major buckwheat allergen (2009)
  • Author(s): Kezuka Y., Itagaki T., Satoh R., Teshima R., Nonaka T.
  • Journal Title: Acta Cryst. F65 Pages: 1267-1270
  • Peer Reviewed
• [Journal Article] Modulation of immunoresponse in BALB/c mice by oral administration of Fag e 1-glucomannan conjugate (2009)
  • Author(s): Suzuki Y., Kassai M., Hirose T., Katayama K., Nakamura K., Akiyama H., Teshima R., Nakamura S.
  • Journal Title: J.Agric.Food Chem. 57 Pages: 9787-9792
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 手島玲子
  • Journal Title: 新開発食品の安全性 3.アレルギー物質を含む食品"食品中の化学物質と安全性"(日本食品衛生協会, 東京) Pages: 150-156
• [Presentation] 食物アレルゲンの消化と抗原性 (2011)
  • Author(s): 手島玲子、中村亮介
  • Organizer: 第61回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2011-11-10
• [Presentation] 食物アレルゲンの消化と抗原性 (2011)
  • Author(s): 手島玲子、中村亮介
  • Organizer: 第61回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京都港区(招待講演)
  • Year and Date: 2011-11-10
• [Presentation] Characterization of food allergen, its prediction, and regulation of food containing allergic ingredients in Japan (2011)
  • Author(s): Teshima R.
  • Organizer: Joint Congress of APAPARI 2011 & 48th JSPACI
  • Place of Presentation: 福岡
  • Year and Date: 2011-10-28
• [Presentation] Characterization of food allergen, its prediction, and regulation of food containing allergic ingredients in Japan (2011)
  • Author(s): Teshima R.
  • Organizer: Joint Congress of APAPARI 2011 & 48th JSPACI
  • Place of Presentation: 福岡市(招待講演)
  • Year and Date: 2011-10-28
• [Presentation] Analysis of T cell epitopes of a major buckwheat allergen, Fag e 2, by using cell-free protein synthesis system (2011)
  • Author(s): Kimura Y., Katayama S., Teshima R., Akiyama H., Nozawa A., Tozawa T., Nakamura S.
  • Organizer: 第9回無細胞科学松山国際シンポジウム
  • Place of Presentation: 松山
  • Year and Date: 2011-09-22
• [Presentation] Analysis of T cell epitopes of a major buckwheat allergen, Fag e 2, by using cell-free protein synthesis system (2011)
  • Author(s): Kimura Y, Katayama S., Teshima R., Akiyama H., Nozawa A., Tozawa T., Nakamura S.
  • Organizer: 第9回無細胞科学松山国際シンポジウム
  • Place of Presentation: 愛媛県松山市
  • Year and Date: 2011-09-22
• [Presentation] マウスの経口食物アレルギーモデルの発症機序:腸管におけるIgA産生の変化 (2011)
  • Author(s): 新藤智子,香取輝美、金澤由基子,小島幸一,手島玲子
  • Organizer: 第18回日本免疫毒性学会学術大会
  • Place of Presentation: 千葉市
  • Year and Date: 2011-09-09
• [Presentation] ソバ主要アレルゲンFag e 2のIgEエピトープの同定 (2010)
  • Author(s): 佐藤里絵、手島玲子
  • Organizer: 第60回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-26
• [Presentation] ソバ主要アレルグンFag e 2のIgEエビトープの同定 (2010)
  • Author(s): 佐藤里絵、手島玲子
  • Organizer: 第60回日本アレルギー学会秋季学術大会
  • Place of Presentation: 千代田区東京
  • Year and Date: 2010-11-26
• [Presentation] アレルゲン研究の最前線 (2009)
  • Author(s): 手島玲子
  • Organizer: 日本食品化学学会
  • Place of Presentation: 東京
  • Year and Date: 2009-05-21
• [Presentation] アレルゲン研究の最前線 (2009)
  • Author(s): 手島玲子
  • Organizer: 日本食品化学学会
  • Place of Presentation: 幕張, 東京
  • Year and Date: 2009-05-21
• [Book] 第2章;食物アレルギーの原因物質他"食物アレルギーA to Z" (2010)
  • Author(s): 手島玲子
  • Total Pages: 312
  • Publisher: 第一出版
• [Book] 新開発食品の安全性3.アレルギー物質を含む食品"食品中の化学物質と安全性" (2010)
  • Author(s): 手島玲子
  • Publisher: 日本食品衛生協会出版
• [Book] 第2章食物アレルギーの原因物質他食物アレルギーA to Z (2010)
  • Author(s): 手島玲子
  • Total Pages: 312
  • Publisher: 第一出版,東京