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Establishment of human cell lines for blood-tissue barrier and application to elucidate mechanism of low-dose anti-cancer drugs

Research Project

Project/Area Number 21590175
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Medical pharmacy
Research InstitutionKeio University

Principal Investigator

NAKASHIMA Emi  慶應義塾大学, 薬学部, 教授 (90115254)

Co-Investigator(Kenkyū-buntansha) TOMI Masatoshi  慶應義塾大学, 薬学部, 准教授 (30334717)
NISHIMURA Tomohiro  慶應義塾大学, 薬学部, 助教 (40453518)
KOSE Noriko  慶應義塾大学, 薬学部, 研究員 (60348612)
Co-Investigator(Renkei-kenkyūsha) SAI Yoshimichi  金沢大学, 大学病院, 准教授 (40262589)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords薬物動態 / 代謝学 / 血管内皮前駆細胞 / 壁細胞 / bFGF / SMA / ケモタキシス / 管腔形成能 / 血管新生阻害 / 抗がん薬 / CD133 / 抗がん剤 / 血管新生 / 腫瘍増殖抑制 / 骨髄由来血管内皮細胞 / 遊走能 / タキサン系抗がん剤 / 微小血管密度低下
Research Abstract

In formation process of a blood-tissue barrier, there is an interaction between specific tissue cells and bone marrow(BM)-derived endothelial progenitor cells(EPC). An assay system using the established cell lines from the specific tissue is desirable to obtain reproducible analysis. By using an established bone marrow-derived vascular endothelium precursor cell line, TR-BME, docetaxel and paclitaxel directly inhibited EPC-initiated vasculogenesis at low(non-cytotoxic) concentrations, causing suppression of tumor growth.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (19 results)

All 2011 2010 2009

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (8 results)

  • [Journal Article] Mother-to-fetus transfer of antiviral drugs and the involvement of transporters at the placental barrier2011

    • Author(s)
      Tomi M, Nishimura T, Nakashima E.
    • Journal Title

      J Pharm Sci

      Volume: 100 Issue: 9 Pages: 3708-3718

    • DOI

      10.1002/jps.22642

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 6-Mercaptopurine transport by equilibrative nucleoside transporters in conditionally immortalized rat syncytiotrophoblast cell lines TR-TBTs2011

    • Author(s)
      Lee NY, Sai Y, Nakashima E, Ohtsuki S, Kang YS.
    • Journal Title

      J Pharm Sci

      Volume: 100 Issue: 9 Pages: 3773-3782

    • DOI

      10.1002/jps.22631

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of zidovudine transfer to molt-4 cells, a human t-cell model, by dehydroepiandrosterone sulfate2011

    • Author(s)
      Nishimura T, Tanaka J, Tomi M, Seki Y, Kose N, Sai Y, Nakashima E.
    • Journal Title

      J Pharm Sci

      Volume: 100 Issue: 9 Pages: 3959-3967

    • DOI

      10.1002/jps.22624

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential Expression of Ezrin and CLP36 in the Two Layers of Syncytiotrophoblast in Rats2010

    • Author(s)
      Higuchi K, Iizasa H, Sai Y, Horieya S, Lee K-E, Wada M, Deguchi M, Nishimura T, Wakayama T, Tamura A, Tsukita S, Kose N, Kang Y-S, Nakashima E.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 33 Issue: 8 Pages: 1400-1406

    • DOI

      10.1248/bpb.33.1400

    • NAID

      130000300367

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proton-coupled erythromycin antiport at rat blood-placenta barrier2010

    • Author(s)
      Sai Y, Nishimura T, Ochi K, Tanaka N, Takagi A, Tomi M, Kose N, Kobayashi Y, Miyakoshi N, Kitagaki S, Mukai C, Nakashima E.
    • Journal Title

      Drug Metab Dispos.

      Volume: 38 Issue: 9 Pages: 1576-1581

    • DOI

      10.1124/dmd.110.033266

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Roles of TauT and system A in cytoprotection of rat syncytiotrophoblast cell line exposed to hypertonic stress2010

    • Author(s)
      Nishimura T, Sai Y, Fujii J, Muta M, Iizasa H, Tomi M, Deureh M, Kose N, Nakashima E.
    • Journal Title

      Placenta

      Volume: 31 Pages: 1003-1009

    • URL

      http://dx.doi.org/10.1016/j.placenta.2010.08.003

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential expression of ezrin and CLP36 in the two layers of syncytiotrophoblast in rats.2010

    • Author(s)
      Higuchi K, Iizasa H, Sai Y, Horieya S, Lee K-E, Wada M, Deguchi M, Nishimura T, Wakayama T, Tamura A, Tsukita S, Kose N, Kang Y-S, Nakashima E.
    • Journal Title

      Biol Pharm Bull

      Volume: 33 Pages: 1400-1406

    • NAID

      130000300367

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proton-coupled erythromycin antiport at rat blood-placenta barrier.2010

    • Author(s)
      Sai Y, Nishimura T, Ochi K, Tanaka N, Takagi A, Tomi M, Kose N, Kobayashi Y, Miyakoshi N, Kitagaki S, Mukai C, Nakashima E.
    • Journal Title

      Drug Metab Dispos

      Volume: 38 Pages: 1576-1581

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of TauT and system A in cytoprotection of rat syncytiotrophoblast cell line exposed to hypertonic stress.2010

    • Author(s)
      Nishimura T, Sai Y, Fujii J, Muta M, Iizasa H, Tomi M, Deureh M, Kose N, Nakashima E.
    • Journal Title

      Placenta

      Volume: 31 Pages: 1003-1009

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of low-dose paclitaxel and docetaxel on endothelial progenitor cells2009

    • Author(s)
      Muta M, Yanagawa T, Sai Y, Saji S, Suzuki E, Aruga T, Kuroi K, Matsumoto G, Toi M, Nakashima E.
    • Journal Title

      Oncology

      Volume: 77 Issue: 3-4 Pages: 182-191

    • DOI

      10.1159/000236016

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of low-dose paclitaxel and docetaxel on endothelial progenitor cells2009

    • Author(s)
      Muta M, Yanagawa T, Sai Y, Saji S, Suzuki E, Aruga T, Kuroi K, Matsumoto G, Toi M, Nakashima E.
    • Journal Title

      Oncology 77

      Pages: 182-191

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] In vivo techniques for the evaluation of passages from Matamal and Fetal sides across the placental bar2011

    • Author(s)
      Asada T, Takanohashi T, Nishimura T, Tomi M, Nakashima E.
    • Organizer
      Asian Federation for Pharmaceutical Sciences 2011
    • Place of Presentation
      Kuala Lumpur Malaysia
    • Related Report
      2011 Final Research Report
  • [Presentation] CD4陽性Tリンパ球モデルMolt-4細胞への抗ウイルス薬ジドブジン取り込み機構と標的化2011

    • Author(s)
      西村友宏、田中純、登美斉俊、巨勢典子、中島恵美
    • Organizer
      日本薬剤学会第26年会
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] ラット胎盤関門細胞株におけるMitoxantroneによるEstrogen Receptorを介したABCG2発現誘導2011

    • Author(s)
      小田憲司、西村友宏、巨勢典子、登美斉俊、中島恵美
    • Organizer
      日本薬剤学会第26年会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] In vivo techniques for the evaluation of passages from Matamal and Fetal sides across the placental barrier2011

    • Author(s)
      Asada T, Takanohashi T, Nishimura T, Tomi M, Nakashima E
    • Organizer
      Asian Federation for Pharmaceutical Sciences 2011
    • Place of Presentation
      Kuala Lumpur Malaysia
    • Related Report
      2011 Annual Research Report
  • [Presentation] ラット胎盤関門細胞株におけるMitoxantroneによるEstrogen Receptorを介したABCG2発現誘導2011

    • Author(s)
      小田憲司、西村友宏、巨勢典子、登美斉俊、中島恵美
    • Organizer
      第6回トランスポーター研究会年会
    • Place of Presentation
      仙台
    • Related Report
      2011 Annual Research Report
  • [Presentation] Ezrin欠損マウス胎盤・胎児で減少するhypotaurineの胎盤におけるトランスポーター同定2010

    • Author(s)
      樋口慧、西村友宏、登美斉俊、若山友彦、田村淳、月田早智子、曽我朋義、崔吉道、中島恵美
    • Organizer
      日本薬剤学会第25年会
    • Place of Presentation
      徳島
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 胎盤hypotaurine取り込みにおけるGABAトランスポーター(Slc6a13)の関与2010

    • Author(s)
      樋口慧、西村友宏、登美斉俊、巨勢典子、崔吉道、中島恵美
    • Organizer
      第5回トランスポーター研究会年会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] ラット胎盤関門におけるエリスロマイシンのプロトン共役排出輸送2010

    • Author(s)
      崔吉道、西村友宏、越智香織、田中紀章、高木彰紀、登美斉俊、巨勢典子、中島恵美
    • Organizer
      第5回トランスポーター研究会年会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report 2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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