Analysis of mtabolome in relation to endocrinological and metabolic diseases by soft ionization mass spectrometry

• Project/Area Number: 21590184
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Kinki University
• Principal Investigator: IKEGAWA Shigeo 近畿大学, 薬学部, 教授 (90111301)
• Co-Investigator(Kenkyū-buntansha): MITAMURA Kuniko 近畿大学, 薬学部, 准教授 (70242526)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 臨床化学 / LC/MS / 胆汁酸 / テトラヒドロコルチコステロイド / グルタチオン / 安定同位元素標識体 / サルフェート / エレクトロスプレーイオン化 / メタボローム / エレクロトスプレーイオン化 / LC / MS / ステロイドホルモン / オキシステロール / N-アセチルシステイン

Research Abstract

Highly sensitive and specific soft ionization mass spectrometry for the determination of tetrahydrocorticosteroids, bile acids, and oxysterols, which are conjugated with glucuronic and sulfuric acids, glutathione, and N-acetylcysteine, have been developed. The developed methods were useful for diagnosis of endocrinological and metabolic diseases.

Research Products

• [Journal Article] Synthesis of the 3-sulfates of S-acyl glutathione conjugated bile acids and their biotransformation by a rat liver cytosolic fraction (2012)
  • Author(s): Mitamura K, Hori N, Mino S, Iida T, Hofmann AF, Ikegawa S
  • Journal Title: Chem. Phys. Lipids Volume: 165 Pages: 261-269
  • Peer Reviewed
• [Journal Article] Synthesis of the 3-sulfates of S-acyl glutathione conjugated bile acids and their biotransformation by a rat liver cytosolic fraction (2012)
  • Author(s): Mitamura K., et al
  • Journal Title: Chem.Phys.Lipids Volume: 165 Issue: 3 Pages: 261-269
  • DOI: 10.1016/j.chemphyslip.2012.02.001
  • Peer Reviewed
• [Journal Article] Identification of S-acyl glutathione conjugates of bile acids in human bile by means of LC/ESI-MS (2011)
  • Author(s): Mitamura K, Hori N, Iida T, Suzuki M, Shimizu T, Nittono H, Takaori K, Takikawa H, Honmann AF, Ikegawa S
  • Journal Title: Steroids Volume: 76 Pages: 1609-11614
  • Peer Reviewed
• [Journal Article] Synthesis of 3-and 21-monosulfates of[2, 2, 3ss, 4, 4-^<2> H_5]-tetra-hydrocorticosteroids in the 5ss-series as internal standards for mass spectrometry (2011)
  • Author(s): Ikegawa S, Nagae K, Mabuchi T, Okihara R, Hasegawa M, Minematsu T, Iida T, Mitamura K
  • Journal Title: Steroids Volume: 76 Pages: 1232-1240
  • Peer Reviewed
• [Journal Article] Chemical synthesis of the(25R)-and (25S)-epimers of 3α, 7α, 12α-trihydroxy-5α-cholestan-27-oic acid as well as their corresponding glycine and taurine conjugates : key intermediates of allo-bile acid biosynthesis from cholesterol (2011)
  • Author(s): Ogawa S, Okada H. Mitamura K. Ikegawa S. Krasowski MD, Hagey LR, Hofmann AF, Iida T
  • Journal Title: Chem. Phys. Lipids Volume: 164 Pages: 368-377
  • Peer Reviewed
• [Journal Article] 液体クロマトグラフィー/質量分析法によるヒト胆汁中グルタチオン抱合型胆汁酸の同定 (2011)
  • Author(s): 池川繁男, 堀直宏, 三田村邦子, 飯田隆, 鈴木光幸, 清水俊明, 入戸野博, 高折恭一
  • Journal Title: 胆膵の病態生理 Volume: 27 Pages: 47-49
  • NAID: 40019262897
  • Peer Reviewed
• [Journal Article] Characterization of non-enzymatic acylation of amino or thiol groups of bionucleophiles by the acyl-adenylate or acyl-CoA thioester of cholic acid (2011)
  • Author(s): Mitamura K, Aoyama E, Sakai T, Iida T, Hofmann AF, Ikegawa S
  • Journal Title: Anal. Bioanal. Chem. Volume: 400 Pages: 2253-2259
  • Peer Reviewed
• [Journal Article] Synthesis of the 3-sulfates of N-acetylcysteine conjugated bile acids(BA-NACs) and their transient formation from BA-NACs and subsequent hydrolysis by a rat liver cytosolic fraction as shown by liquid chromatography/electrosprayion-ization-mass spectromet (2011)
  • Author(s): Mitamura K, Sakai T, Nakai R, Wakamiya T, Iida T, Hofmann AF, Ikegawa S
  • Journal Title: Anal. Bioanal. Chem. Volume: 400 Pages: 2061-2072
  • Peer Reviewed
• [Journal Article] Stable isotope-dilution liquid chromatography/tandem mass spectrometry method for determination of thyroxine in saliva (2011)
  • Author(s): Higashi T, Ichikawa T, Shimizu C, Nagai S, Inagaki S, Min JZ, Chiba H, Ikegawa S, Toyo oka T
  • Journal Title: J. Chromatogr. B Volume: 879 Pages: 1013-1017
  • Peer Reviewed
• [Journal Article] Chemical synthesis of the 17-propanamide derivatives of stereoisomericΔ^<14>-17α-and 17ss-estradiols : potential 17ss-hydroxysteroid dehydrogenase inhibitors (2011)
  • Author(s): Iida T, Ogawa S, Tamegai H, Adachi Y, Saito H, Ikegawa S, Konishi H, Takagi A, Matsuzaki T
  • Journal Title: Chem. Phys. Lipids Volume: 164 Pages: 106-112
  • Peer Reviewed
• [Journal Article] Identification of bile acid S-acyl glutathione conjugates in rat bile by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry (2011)
  • Author(s): Mitamura K, Hori N, Iida T, Hofmann AF, Ikegawa S
  • Journal Title: Steroids Volume: 76 Pages: 68-77
  • Peer Reviewed
• [Journal Article] Stable isotope-dilution liquid chromatography/tandem mass spectrometry method for determination of thyroxine in saliva (2011)
  • Author(s): Higashi T., et al
  • Journal Title: J.Chromatogr.B Volume: 879 Issue: 13-14 Pages: 1013-1017
  • DOI: 10.1016/j.jchromb.2011.02.048
  • Peer Reviewed
• [Journal Article] Synthesis of the 3-sulfates of N-acetylcysteine conjugated bile acids (BA-NACs) and their transient formation from BA-NACs and subsequent hydrolysis by a rat liver cytosolic fraction as shown by liquid chromatography/electrospray ionization-mass spectrometry (2011)
  • Author(s): Mitamura K., et al
  • Journal Title: Anal.Bioanal.Chem. Volume: 400 Issue: 7 Pages: 2061-2072
  • DOI: 10.1007/s00216-011-4925-3
  • Peer Reviewed
• [Journal Article] Characterization of non-enzymatic acylation of amino or thiol groups of bionucleophiles by the acyl-adenylate or acyl-CoA thioester of cholic acid (2011)
  • Author(s): Mitamura K., et al
  • Journal Title: Anal.Bioanal.Chem. Volume: 400 Issue: 7 Pages: 2253-2259
  • DOI: 10.1007/s00216-011-4961-z
  • Peer Reviewed
• [Journal Article] Chemical synthesis of the (25R)- and (25S)-epimers of 3α,7α,12α-trihydroxy-5α-cholestan-27-oic acid as well as their corresponding glycine and taurine conjugates : key intermediates of allo-bile acid biosynthesis from cholesterol (2011)
  • Author(s): Ogawa S., et al
  • Journal Title: Chem.Phys.Lipids Volume: 164 Issue: 5 Pages: 368-377
  • DOI: 10.1016/j.chemphyslip.2011.04.008
  • Peer Reviewed
• [Journal Article] Synthesis of 3- and 21-monosulfates of [2,2,3β,4,4-^2H_5]-tetrahydrocorticosteroids in the 5β-series as internal standards for mass spectrometry (2011)
  • Author(s): Ikegawa S., et al
  • Journal Title: Steroids Volume: 76 Issue: 12 Pages: 1232-1240
  • DOI: 10.1016/j.steroids.2011.05.014
  • Peer Reviewed
• [Journal Article] Identification of S-acyl glutathione conjugates of bile acids in human bile by means of LC/ESI-MS (2011)
  • Author(s): Mitamura K., et al
  • Journal Title: Steroids Volume: 76 Issue: 14 Pages: 1609-1614
  • DOI: 10.1016/j.steroids.2011.10.001
  • Peer Reviewed
• [Journal Article] Identification of bile acid S-acyl glutathione conjugates in rat bile by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry. (2011)
  • Author(s): Mitamura K., et al
  • Journal Title: Steroids Volume: 76 Pages: 68-77
  • Peer Reviewed
• [Journal Article] Chemical Synthesis of the 17-Propanamide Derivatives of Stereoiomeric △^<14>-17α-and 17β-Estradiols : Potential 17β-Hydroxysteroid Dehydrogenase Inhibitors. (2011)
  • Author(s): Iida T., et al.
  • Journal Title: Chem.Phys.Lipids Volume: 164 Pages: 106-112
  • Peer Reviewed
• [Journal Article] Simultaneous determination of tetrahydrocorticosteroid glucuronide by liquid chromatography/electrospray ionization-mass spectrometry (2010)
  • Author(s): Mitamura K, Ikegawa S
  • Journal Title: Korean J. Lab. Med Volume: 30
• [Journal Article] Salivary cheno-deoxycholic acid and its glycine-conjugate : Their determination method using LC-MS/MS and variation of their concentrations with increased saliva flow rate (2010)
  • Author(s): Higashi T, Shibayama Y. Ichikawa T, Ito K, Toyoka T, Shimada K : Mitamura K, Ikegawa S, Chiba H
  • Journal Title: Steroids Volume: 75 Pages: 338-345
  • Peer Reviewed
• [Journal Article] LC-MS/MS coupled with immunoaffinity extraction for determination of estrone, 17ss-estradiol and estrone 3-sulfate in human plasma (2010)
  • Author(s): Hosogi J, Tanaka H, Fujita K, Kuwabara T, Ikegawa S, Kobayashi N, Mano N, Goto J
  • Journal Title: J. Chromatogr. B Volume: 878 Pages: 222-227
  • Peer Reviewed
• [Journal Article] Potential corticoid metabolites : chemical synthesis of 3-and 21-mono-and 3, 21-disulfate conjugates of tetrahydrocorticosteroids in the 5α-and 5ss-series (2010)
  • Author(s): Okihara R, Mitamura K, Hasegawa M, Mori M, Muto A, Kakiyama G, Iida T, Shimada M, Mano N, Ikegawa S
  • Journal Title: Chem. Pharm. Bull Volume: 58 Pages: 344-353
  • Peer Reviewed
• [Journal Article] Potential Corticoid Metabolites : Chemical Synthesis of 3- and 21-Mono- and 3,21-Disulfate Conjugates of Tetrahydrocorticosteroids in the 5α- and 51β-Series. (2010)
  • Author(s): Okihara R., et al
  • Journal Title: Chem.Pharm.Bull Volume: 58 Pages: 344-353
  • Peer Reviewed
• [Journal Article] Salivary chenodeozycholic acid and its glycine-conjugate : Their determination method using LC-MS/MS and validation of their concentration with increased saliva flow rate. (2010)
  • Author(s): Higashi T. , et al
  • Journal Title: Steroids Volume: 75 Pages: 338-345
  • Peer Reviewed
• [Journal Article] Simultaneous determination of tetrahydrocorticosteroid glucuronides by liquid chromatography/electrospray, ionization-linear ion trap mass spectrometry. (2010)
  • Author(s): Mitamura K., et al
  • Journal Title: Korean.J.Lab.Med. Volume: 30
• [Journal Article] Potential Corticoid Metabolites : Chemical Synthesis of 3- and 21-Mono- and 3, 21-Disulfate Conjugates of Tetrahydrocorticosteroids in the 5α- and 5β-Series (2010)
  • Author(s): Okihara R., et al.
  • Journal Title: Chem.Pharm.Bull 58 Pages: 344-353
  • Peer Reviewed
• [Journal Article] Salivary chenodeoxycholic acid and its glycine-conjugate : Their determination method using LC-MS/MS and variation of their concentrations with increased saliva flow rate (2010)
  • Author(s): Higashi T., et al.
  • Journal Title: Steroids 75 Pages: 338-345
  • Peer Reviewed
• [Journal Article] Simultaneous determination of twelve tetrahydrocorticosteroid glucuronide in human urine by liquid chromatography/electrospray ionization iinear ion trap mass spectrometry (2009)
  • Author(s): Ikegawa S, Hasegawa M, Okihara R, Shimizu C, Chiba H, Iida T, Mitamura K
  • Journal Title: Anal. Chem. Volume: 81 Pages: 10124-10135
  • Peer Reviewed
• [Journal Article] 胆汁酸のグルタチオン抱合体とLC/MS (2009)
  • Author(s): 三田村邦子, 堺俊博, 池川繁男, 若宮建昭
  • Journal Title: JUSCO Report Volume: 51 Pages: 7-13
  • NAID: 40016867163
• [Journal Article] LC/ESI-MSによる内分泌・代謝疾患の科学診断へのアプローチ (2009)
  • Author(s): 三田村邦子, 長谷川真紀, 沖原梨香, 池川繁男
  • Journal Title: 臨床化学 Volume: 38 Pages: 291-298
  • NAID: 10025633137
  • Peer Reviewed
• [Journal Article] Chemical synthesis of N-acetylcysteine conjugates of bile acids and in vivo formation in cholestatic rats as shown by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry (2009)
  • Author(s): Mitamura K, Watanabe S, Sakai T, Okihara R, Sogabe M, Wakamiya T, Hofmann AF, Ikegawa S
  • Journal Title: J. Chromatogr. B. Volume: 877 Pages: 2630-2638
  • NAID: 10028022525
  • Peer Reviewed
• [Journal Article] Chemical Synthesis of bile acid acyl-adenylates and formation by rat liver microsomal fraction (2009)
  • Author(s): Ikegawa S, Ito H, Ohshima M, Maeda M, Hofmann AF, Mitamura K
  • Journal Title: Steroids Volume: 74 Pages: 751-757
  • Peer Reviewed
• [Journal Article] Formation and biliary excretion of glutathione conjugates of bile acids in the rat as shown by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry (2009)
  • Author(s): Mitamura K, Watanabe S, Mitsumoto Y, Sakai T, Sogabe M, Wakamiya T, Ikegawa S
  • Journal Title: Anal. Biochem. Volume: 384 Pages: 224-230
  • Peer Reviewed
• [Journal Article] A facile Synthesis of C-24 and C-25 oxysterols by in situ generated ethyl(tri-fluoromethyl) dioxirane (2009)
  • Author(s): Ogawa S, Kakiyama G, Muto A, Hosoda A, Mitamura K, Ikegawa S, Hofmann AF, Iida T
  • Journal Title: Steroids Volume: 74 Pages: 81-87
  • Peer Reviewed
• [Journal Article] Simultaneous Determination of Twelve Tetrahydrocorticosteroid Glucuronide in Human Urine by Liquid Chromatopraphy/Electrospray Ionization Linear Ion Trap Mass Spectrometry (2009)
  • Author(s): Ikegawa S., et al.
  • Journal Title: Anal.Chem. 81 Pages: 10124-10135
  • Peer Reviewed
• [Journal Article] LC-MS/MS coupled with immunoaffinity extraction for determination of estrone, 17b-estradiol and estrone 3-sulfate in human plasma (2009)
  • Author(s): Hosogi J., et al.
  • Journal Title: J.Chroamtogr.B 878 Pages: 222-227
  • Peer Reviewed
• [Journal Article] Formation and biliary excretion of glutathione conjugates of bile acids in the rat as shown by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry (2009)
  • Author(s): 三田村邦子., et al.
  • Journal Title: Anal.Biochem. 384 Pages: 224-230
  • Peer Reviewed
• [Journal Article] Chemical synthesis of N-acetylsysteine conjugates of bile acids and in vivo formation in cholestatic rats as shown by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry (2009)
  • Author(s): 三田村邦子., et al.
  • Journal Title: J.Chromatogr.B 877 Pages: 2630-2638
  • Peer Reviewed
• [Journal Article] Chemical Synthesis of bile acid acyl-adenylates and formation by rat liver microsomal fraction (2009)
  • Author(s): Ikegawa S., et al.
  • Journal Title: Steroids 74 Pages: 751-757
  • Peer Reviewed
• [Journal Article] LC/ESI-MSによる内分泌・代謝疾患の科学診断へのアプローチ (2009)
  • Author(s): 三田村邦子, et al.
  • Journal Title: 臨床化学 38 Pages: 291-298
  • NAID: 10025633137
  • Peer Reviewed
• [Journal Article] 胆汁酸のグルタチオン抱合とLC/MS (2009)
  • Author(s): 三田村邦子, et al.
  • Journal Title: Jasco Report 51 Pages: 7-13
• [Journal Article] (2009)
  • Author(s): 池川繁男
  • Journal Title: 薬学分析科学の最前線(じほう) Pages: 130-131
• [Presentation] 同位体希釈LC/ESI-MSに用いる多重重水素標識A/B-トランスallo-tetrahydrocorticosteroid 3-及び21-monosulfateの合成 (2012)
  • Author(s): 池川繁男
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2012-03-30
• [Presentation] テトラヒドロコルチコステロイドのグルクロン酸抱合に関する検討 (2012)
  • Author(s): 三田村邦子
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2012-03-29
• [Presentation] オオトカゲ胆嚢胆汁成分から新規胆汁酸の単離・同定:Tauro-(24R,25S)-3α,7α,12α,24-tetrahydroxy-5β-cholestan-27-oic acid (2012)
  • Author(s): 小川祥二郎
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2012-03-29
• [Presentation] グルタミン抱合型胆汁酸の高感度測定法開発に関する基礎的検討 (2012)
  • Author(s): 池川繁男
  • Organizer: 第21回日本小児胆汁酸研究会
  • Place of Presentation: 東京
  • Year and Date: 2012-02-18
• [Presentation] グルタミン抱合型胆汁酸の高感度測定法開発に関する基礎的検討 (2012)
  • Author(s): 池川繁男
  • Organizer: 第21回日本小児胆汁酸研究会
  • Place of Presentation: 山の上ホテル(東京)
  • Year and Date: 2012-02-18
• [Presentation] 同位体希釈LC/ESI-MSに用いる多重重水素標識A/B-トランスallo-tetrahydro-corticosteroid 3-及び21-monosulfateの合成 (2012)
  • Author(s): 池川繁男
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] オオトカゲ胆嚢胆汁成分から新規胆汁酸の単離・同定: Tauro-(24R, 25S)-3α, 7α, 12α, 24-tetrahydroxy-5ss-cholestan-27-oic acid (2012)
  • Author(s): 小川祥二郎
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] テトラヒドロコルチコステロイドのグルクロン酸抱合に関する検討 (2012)
  • Author(s): 三田村邦子
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] Allo-tetrahydro-11-deoxycortisol 3-及び21-monosulfateの同位体希釈LC/ESI-MSに用いる多重重水素標識体の合成 (2011)
  • Author(s): 池川繁男
  • Organizer: 第61回日本薬学会近畿支部総会・大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-10-22
• [Presentation] Allo-tetrahydro-11-deoxycortisol 3-及び21-monosulfateの同位体希釈LC/ESI-MSに用いる多重重水素標識体の合成 (2011)
  • Author(s): 中嶋理陽
  • Organizer: 第61回日本薬学会近畿支部総会・大会
  • Place of Presentation: 神戸大学(神戸)
  • Year and Date: 2011-10-22
• [Presentation] LC/MSによるグルタチオン抱合型胆汁酸の動態解析 (2011)
  • Author(s): 三田村邦子
  • Organizer: 第60回日本分析化学会
  • Place of Presentation: 名古屋大学(名古屋)
  • Year and Date: 2011-09-15
• [Presentation] ラット胆汁中グルタチオン抱合型フェニル酪酸のLC/ESI-MS/MSによる同定 (2011)
  • Author(s): 池川繁男
  • Organizer: 第36回日本医用マススペクトル学会年会
  • Place of Presentation: ホテル阪急(大阪)
  • Year and Date: 2011-09-15
• [Presentation] グルタチオン抱合型胆汁酸の胆汁排泄 (2011)
  • Author(s): 三田村邦子
  • Organizer: 第51回日本臨床化学会年次学術集会
  • Place of Presentation: 札幌医科大学(札幌)
  • Year and Date: 2011-08-27
• [Presentation] ソフトイオン化質量分析法によるグルタチオン抱合型胆汁酸の生成と動態解析 (2011)
  • Author(s): 池川繁男
  • Organizer: 第51回日本臨床化学会年次学術集会
  • Place of Presentation: 札幌医科大学(札幌)
  • Year and Date: 2011-08-27
• [Presentation] N-アセチルシステイン抱合型ウルソデオキシコール酸の体内動態と肝機能完全作用に関する基礎的検討 (2011)
  • Author(s): 三田村邦子
  • Organizer: 第20回西日本臨床胆汁酸研究会
  • Place of Presentation: 大阪
  • Year and Date: 2011-07-23
• [Presentation] N-アセチルシステイン抱合型ウルソデオキシコール酸の体内動態と肝機能完全作用に関する基礎的検討 (2011)
  • Author(s): 三田村邦子
  • Organizer: 第20回西日本臨床胆汁酸研究会
  • Place of Presentation: リーガロイヤルホテル(大阪)
  • Year and Date: 2011-07-23
• [Presentation] グルタチオン抱合型胆汁酸3-サルフェートの合成とラット肝サイトゾール画分における生成 (2011)
  • Author(s): 三田村邦子
  • Organizer: 第28回日本胆膵病態生理研究会
  • Place of Presentation: 福岡
  • Year and Date: 2011-06-18
• [Presentation] グルタチオン抱合型胆汁酸3-サルフェートの合成とラット肝サイトゾール画分における生成 (2011)
  • Author(s): 三田村邦子
  • Organizer: 第28回日本胆膵病態生理研究会
  • Place of Presentation: 福岡大学(福岡)
  • Year and Date: 2011-06-18
• [Presentation] Liquid chromatography-mass spectrometric characterization of sulfation of N-acetylcysteine conjugated bile acids by a rat liver cytosolic fraction (2011)
  • Author(s): Shigeo Ikegawa
  • Organizer: 59th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: デンバー(米国)
  • Year and Date: 2011-06-06
• [Presentation] Liquid chromatography/mass spectrometric characterization of non-enzymatic acylation of amino or thiol groups of bionucleophiles by the cholyl-adenylate or cholyl-CoA thioester (2011)
  • Author(s): Kuniko Mitamura
  • Organizer: 59th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: デンバー(米国)
  • Year and Date: 2011-06-06
• [Presentation] LC/ESI-MS/MSによるヒト胆汁中グルタチオン抱合型胆汁酸の同定 (2011)
  • Author(s): 三田村邦子
  • Organizer: 第21回日本臨床化学会近畿支部総会
  • Place of Presentation: 大阪
  • Year and Date: 2011-02-05
• [Presentation] LC/MSによるヒト胆汁中グルタチオン抱合型胆汁酸の同定 (2011)
  • Author(s): 三田村邦子
  • Organizer: 第21回日本臨床化学会近畿支部総会
  • Place of Presentation: 大阪
  • Year and Date: 2011-02-05
• [Presentation] ラット胆汁中グルタチオン抱合型フェニル酪酸のLC/ESI-MS/MSによる同定 (2011)
  • Author(s): 池川繁男
  • Organizer: 第36回日本医用マススペクトル学会年会
  • Place of Presentation: 大阪
• [Presentation] LC/MSによるグルタチオン抱合型胆汁酸の動態解析 (2011)
  • Author(s): 三田村邦子
  • Organizer: 第60回日本分析化学会
  • Place of Presentation: 名古屋
• [Presentation] ソフトイオン化質量分析法によるグルタチオン抱合型胆汁酸の生成と動態解析 (2011)
  • Author(s): 池川繁男
  • Organizer: 第51回日本臨床化学会年次学術集会
  • Place of Presentation: 札幌
• [Presentation] グルタチオン抱合型胆汁酸の胆汁排泄 (2011)
  • Author(s): 三田村邦子
  • Organizer: 第51回日本臨床化学会年次学術集会
  • Place of Presentation: 札幌
• [Presentation] LC/ESI-MS/MSによるヒト胆汁中グルタチオン抱合型胆汁酸の同定 (2011)
  • Author(s): 池川繁男
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
• [Presentation] グルタチオン抱合型胆汁酸3-サルフェートの合成とラット肝サイトゾール画分における生成 (2011)
  • Author(s): 三田村邦子
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
• [Presentation] LC/ESI-MS/MSによるヒト胆汁中グルタチオン抱合型胆汁酸の同定 (2010)
  • Author(s): 池川繁男
  • Organizer: 第32回胆汁酸研究会
  • Place of Presentation: 仙台
  • Year and Date: 2010-11-09
• [Presentation] グルタチオン抱合型胆汁酸のラット肝サイトゾール画分における硫酸抱合の解析 (2010)
  • Author(s): 池川繁男
  • Organizer: 第32回胆汁酸研究会
  • Place of Presentation: 仙台
  • Year and Date: 2010-11-09
• [Presentation] 胆汁酸のグルタチオン抱合体と胆汁排泄 (2010)
  • Author(s): 池川繁男
  • Organizer: 第27回日本胆膵病態・生理研究会
  • Place of Presentation: 弘前
  • Year and Date: 2010-06-26
• [Presentation] Mass Spectrometric Techniques Applied to the Study of Conjugated Bile Acids, Steroids, and Oxysterols. Research Seminar at Virginia Commonwealth Uni-versity (2010)
  • Author(s): Ikegawa S
  • Organizer: Medical College of Virginia Campus
  • Place of Presentation: Richmond, USA
  • Year and Date: 2010-05-27
• [Presentation] Recent application of mass spectrometry to the metabolic studies of bile acids, steroids, and oxysterol (2010)
  • Author(s): Ikegawa S.
  • Organizer: Research Seminor at Virginia Commonwealth University
  • Place of Presentation: リッチモンド・米国
  • Year and Date: 2010-05-26
• [Presentation] 重水素標識N-アセチルシステインによる胆汁酸代謝活性中間体のトラッピング (2010)
  • Author(s): 池川繁男
  • Organizer: 第19回日本小児胆汁酸研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-02-20
• [Presentation] 肝・胆・消化機能改善作用を指向したプロドラッグ開発の基礎的検討:重水素標識N-アセチルシステイン抱合型ウルソデオキシコール酸の体内動態の追跡 (2010)
  • Author(s): 三田村邦子
  • Organizer: 第19回日本小児胆汁酸研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-02-20
• [Presentation] Analysis of glutathione conjugates of bile acids in rat bile by LC/ESI-MS/MS (2010)
  • Author(s): Ikegawa S
  • Organizer: XXI International Bile Acid Meeting
  • Place of Presentation: Freiburg, Germany
• [Presentation] Chemical synthesis of the(25R)-and (25S)-epimers of 3□, 7□, 12□-trihydroxy-5□-cholestan-27-oic acid and their glycine and taurine conjugates (2010)
  • Author(s): Iida T
  • Organizer: XXI International Bile Acid Meeting
  • Place of Presentation: Freiburg, Germany
• [Presentation] Simultaneous determination of tetrahydrocorticosteroid glucuronides by liquid chromatography/electrospray ioniza-tion-mass spectrometry (2010)
  • Author(s): Mitamura K
  • Organizer: The 12th Asian-Pacific Congress of Clinical Biochemistry
  • Place of Presentation: Seoul, Korea
• [Presentation] ヒト血中硫酸抱合型オキシステロールの同位体希釈LC/ESI-MSによる直接一斉分析法の開発 (2010)
  • Author(s): 坂野理絵
  • Organizer: 第50回日本臨床化学会年次学術集会
  • Place of Presentation: 甲府
• [Presentation] イオンクラスター法による胆汁酸代謝活性中間体のチオール基並びにアミノ基との反応性の解析 (2010)
  • Author(s): 三田村邦子
  • Organizer: 第35回日本医用マススペクトル学会年会
  • Place of Presentation: 名古屋
• [Presentation] 内分泌・代謝疾患のLC/MSによるメタボロミクスに用いる多重重水素標識A/B-シス-テトラヒドロコルチコステロイド・サルフェートの合成 (2010)
  • Author(s): 池川繁男
  • Organizer: 第23回バイオメディカル分析科学シンポジウム
  • Place of Presentation: 松島
• [Presentation] Quantitative metabolic profiling of twelve tetrahyrocorticosteroid glucur-onides in urine by isotope dilution LC-ESI-linear ion trap MS/MS (2010)
  • Author(s): Mitamura K
  • Organizer: 58th ASMS Conference on Mass Spectrometry
  • Place of Presentation: Salt Lake City, USA
• [Presentation] Identification of novel metabolites of bile acids : S-acyl glutathione conjugates in rat bile by LC/ESI-linear ion trap MS/MS (2010)
  • Author(s): Hori N
  • Organizer: 58th ASMS Conference on Mass Spectrometry
  • Place of Presentation: Salt Lake City, USA
• [Presentation] ラット胆汁中グルタチオン抱合型胆汁酸の合成とLC/MSによる同定 (2010)
  • Author(s): 堀直宏
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
• [Presentation] LC/ESI-MS/MSによるラット胆汁中重水素標識N-アセチルシステイン抱合型胆汁酸の同定 (2010)
  • Author(s): 堺俊博
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
• [Presentation] LC/ESI-MS/MSによるヒト尿中テトラヒドロ-並びにヘキサヒドロコルチコステロイド・グルクロニド直接一斉分析法の開発 (2010)
  • Author(s): 三田村邦子
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
• [Presentation] LC/ESI-MS/MSによるN-アセチルシステイン抱合型ウルソデオキシコール酸の体内動態に関する基礎的検討 (2010)
  • Author(s): 池川繁男
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
• [Presentation] Quantitative Metabolic Profiling of Twelve Tetrahyrocorticosteroid Glucuronides in Urine by Isotope Dilution LC-ESI-Linear Ion Trap MS/MS. (2010)
  • Author(s): Mitamura K.
  • Organizer: 58th ASMS Conference on Mass Spectrometry
  • Place of Presentation: ソルトレイクシティー・米国
• [Presentation] Identification of Novel Metabolites of Bile Acids : S-Acyl Glutathionc Conjugates in Rat Bile by LC/ESI-Linear Ion Trap MS/MS. (2010)
  • Author(s): Hori N.
  • Organizer: 58th ASMS Conference on Mass Spectrometry
  • Place of Presentation: ソルトレイクシティー・米国
• [Presentation] 内分泌・代謝疾思のLC/MSによるメタボロミクスに用いる多重重水素標識A/B-シスーテトラヒドロコルチコステロイド・サルフェートの合成 (2010)
  • Author(s): 池川繁男
  • Organizer: 第23回バイオメディカル分析科学シンポジウム
  • Place of Presentation: 松島
• [Presentation] ヒト血中硫酸抱合型オキシステロールの同位体希釈LC/ESI-MSによる直接-斉分析法の開発 (2010)
  • Author(s): 坂野理絵
  • Organizer: 第50回日本臨床化学会年次学術集会
  • Place of Presentation: 甲府
• [Presentation] Simultaneous Determination of Tetrahydrocorticosteroid Glucuronides by Liquid Chromatography/Electrospray Ionization-Mass Spectrometry (2010)
  • Author(s): Mitamura K.
  • Organizer: The 12th Asian-Pacific Congress of Clinical Biochemistry
  • Place of Presentation: ソウル・韓国
• [Presentation] Chemical synthesis of the (25R)- and (25S)-epimers of 3α,7α,12α-trihydroxy-5α-cholestan-27-oic acid and their glycine and taurine conjugates. (2010)
  • Author(s): Iida T.
  • Organizer: XXI International Bile Acid
  • Place of Presentation: フライブルク・ドイツ
• [Presentation] Analysis of glutathione conjugates of bile acids in rat bile by LC/ESI-MS/MS (2010)
  • Author(s): Ikegawa S.
  • Organizer: XXI International Bile Acid Meeting
  • Place of Presentation: フライブルク・ドイツ
• [Presentation] LC/ESI-MS/MSによるヒト尿中テトラヒドロ-並びにヘキサヒドロコルチコステロイド・グルクロニド直接-斉分析法の開発 (2010)
  • Author(s): 三田村邦子
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
• [Presentation] 肝・胆・消化機能改善薬として期待されるN-アセチルシステイン抱合型ウルソデオキシコール酸の体内動態に関する基礎的研究 (2009)
  • Author(s): 池川繁男
  • Organizer: 第20回日本臨床化学会近畿支部総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-26
• [Presentation] 質量分析(LC/MS)と臨床化学 (2009)
  • Author(s): 池川繁男
  • Organizer: 第27回日本臨床化学会関東支部例会
  • Place of Presentation: 東京
  • Year and Date: 2009-12-05
• [Presentation] ラット胆汁中グルタチオン抱合体の合成とこれらのLC/MSによる同定 (2009)
  • Author(s): 堀直宏
  • Organizer: 第31回胆汁酸研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-07
• [Presentation] ラット胆汁中グルタチオン抱合体の合成とこれらのLC/MSによる同定 (2009)
  • Author(s): 堀直宏
  • Organizer: 第31回胆汁酸研究会
  • Place of Presentation: 大阪
  • Year and Date: 2009-11-07
• [Presentation] 多重重水素標識テトラヒドロコルチコステロイド・サルフェートの合成とESI-MS^nにおける挙動 (2009)
  • Author(s): 三田村邦子
  • Organizer: 第59回日本薬学会近畿支部大会
  • Place of Presentation: 大阪
  • Year and Date: 2009-10-24
• [Presentation] LC/MSによる硫酸抱合型オキシステロール直接一斉分析法の開発 (2009)
  • Author(s): 坂野理絵
  • Organizer: 第59回日本薬学会近畿支部大会
  • Place of Presentation: 大阪
  • Year and Date: 2009-10-24
• [Presentation] LC/ESI-MS/MSによるN-アセチルシステイン抱合型胆汁酸3-サルフェートのトレースアナリシス (2009)
  • Author(s): 池川繁男
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 札幌
  • Year and Date: 2009-09-24
• [Presentation] LC/ESI-MS/MSによるラット並びにヒト胆汁中グルタチオン抱合型胆汁酸の解析 (2009)
  • Author(s): 三田村邦子
  • Organizer: 第19回西日本臨床胆汁酸研究会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-25
• [Presentation] Identification of glutathione conjugates of bile acids in rat bile by LC/ESI-MS^2 (2009)
  • Author(s): Ikegawa S
  • Organizer: 日本薬物動態学会第24回年会
  • Place of Presentation: 京都
• [Presentation] 同位体希釈LC/ESI-MS/MSによる尿中テトラヒドロコルチコステロイド・サルフェート定量法の開発 (2009)
  • Author(s): 三田村邦子
  • Organizer: 第49回日本臨床化学会年会
  • Place of Presentation: 長崎
• [Presentation] 唾液中胆汁酸: LC-ESI-MS/MS定量法と唾液分泌量亢進に伴う濃度変動 (2009)
  • Author(s): 東達也
  • Organizer: 第34回日本医用マススペクトル学会年会
  • Place of Presentation: 大阪
• [Presentation] LC/ESI-MS/MSによるN-アセチルシステイン抱合型胆汁酸のラット肝サイトゾール画分における硫酸抱合の解析 (2009)
  • Author(s): 堺俊博
  • Organizer: 第34回日本医用マススペクトル学会年会
  • Place of Presentation: 大阪
• [Presentation] ラット胆汁中グルタチオン抱合型胆汁酸の同定 (2009)
  • Author(s): 堀直宏
  • Organizer: 第34回日本医用マススペクトル学会年会
  • Place of Presentation: 大阪
• [Presentation] 胆汁酸のグルタチオン抱合と胆汁排泄 (2009)
  • Author(s): 池川繁男
  • Organizer: 第22回バイオメディカル分析科学シンポジウム
  • Place of Presentation: 岐阜
• [Presentation] LC/ESI-MS/MSによるN-アセチルシステイン抱合型胆汁酸のラット肝サイトソール画分における硫酸抱合の解析 (2009)
  • Author(s): 堺俊博
  • Organizer: 第34回日本医用マススペクトル学会年会
  • Place of Presentation: 大阪
• [Presentation] 唾液中胆汁酸:LC-ESI-MS/MS定量法と唾液分泌量亢進に伴う濃度変動 (2009)
  • Author(s): 東達也
  • Organizer: 第34回日本医用マススペクトル学会年会
  • Place of Presentation: 大阪
• [Presentation] 同位体希釈LC/ESI-MS/MSによる尿中テトラヒドロコルチコステロイド・サルフェート定量法の開発 (2009)
  • Author(s): 三田村邦子
  • Organizer: 第49回日本臨床化学会年次学術集会
  • Place of Presentation: 長崎
• [Presentation] LC/ESI-MS/MSによるN-アセチルシステイン抱合型胆汁酸3-サルフェートのトレースアナリシス (2009)
  • Author(s): 池川繁男
  • Organizer: 日本分析化学会第59年会
  • Place of Presentation: 札幌
• [Presentation] N-アセチルシステインとの抱合体形成による胆汁酸の新規代謝経路の解明に関する研究 (2009)
  • Author(s): 中井莉沙
  • Organizer: 第46回ペプチド討論会
  • Place of Presentation: 飯塚
• [Presentation] Identification of Glutathione Conjugates of Bile Acids in Rat Bile by LC/ESI-MS^2 (2009)
  • Author(s): 池川繁男
  • Organizer: 日本薬物動態学会第24回年会
  • Place of Presentation: 京都
• [Presentation] Liquid chromatography/mass spectrometric characterization of non-enzymatic acylation of amino or thiol groups of bionucleophiles by the cholyl-adenylate or cholyl-CoA thioester
  • Author(s): Kuniko Mitamura
  • Organizer: 59th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Denver, CO, USA
• [Presentation] Liquid chromatography-mass spectrometric characterization of sulfation of N-acetylcysteine conjugated bile acids by a rat liver cytosolic fraction
  • Author(s): Shigeo Ikegawa
  • Organizer: 59th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Denver, CO, USA
• [Book] 試料分析講座脂質分析 (2012)
  • Author(s): 池川繁男, 三田村邦子
  • Publisher: 丸善出版
• [Book] 試料分析講座脂質分析 (2011)
  • Author(s): 池川繁男
  • Publisher: 丸善出版
• [Book] 薬学生のための臨床化学 (2010)
  • Author(s): 池川繁男, 三田村邦子
  • Publisher: 南江堂
• [Book] 薬学生のための臨床化学(改訂第3版) (2010)
  • Author(s): 池川繁男
  • Publisher: 南江堂
• [Book] 薬学分析科学の最前線 (2009)
  • Author(s): 池川繁男, 三田村邦子
  • Publisher: じほう