| Project/Area Number |
21590198
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UEYAMA Takashi 和歌山県立医科大学, 医学部, 准教授 (50264875)
ITO Takao 和歌山県立医科大学, 医学部, 助教 (30315931)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | RNAヘリカーゼ / 神経系 / オリゴデンドロサイト / 遺伝子改変動物 / 分子形態学 |
|---|
| Research Abstract |
The monoclonal antibody(4F2) that specifically identifies oligodendrocytes was generated, and the molecule recognized by this antibody proved to be Ddx54, a member of RNA helicase. In the transgenic animals with forced expression of Ddx54, myelination appeared to be accelerated. The 4F2-positive cells were observed in the rat central nervous system from early embryonal to adult stages, and they were specifically expressed in oligodendrocytes using primary cultures of neural tissues. Ddx54 bound to 4 types of MBP isoforms, and especially the 21. 5 kDa isoform of MBP was transported into the nucleus, suggesting its involvement in the signal transduction during the process of myelination in oligodendrocytes.
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