Roles of transcription factors during endothelial-mesenchymal transformation in the developing chick heart

• Project/Area Number: 21590201
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Osaka City University
• Principal Investigator: YAMAGISHI Toshiyuki 大阪市立大学, 大学院・医学研究科, 講師 (60255122)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 心臓発生 / 心内膜床 / 上皮-間葉形質転換 / 転写因子 / sox9 / 心内膜症

Research Abstract

I examined role of sox9 in the endothelial-mesenchymal transformation(EMT) during cushion tissue formation using chick embryonic hearts. First, we examined their expression patterns in the developing heart by in situ hybridization. sox9 was expressed in the activated-endothelial and mesenchymal cells of the outflow tract and the atrioventricular canal regions. Second, sox9 siRNA inhibited endothelial transformation in vitro assay. Endothelial cells transfected with sox9 showed cell migration, but did not invade into the gel lattice. However endothelial cells transfected with sox9 and slug transformed to mesenchymal cells. Taken together, results suggest that sox9 and slug may play an important role during endocardial epithelial-mesenchymal transition

Research Products

• [Journal Article] Expression of the Tgfβ2 gene during chick embryogenesis (2012)
  • Author(s): Yamagishi T, Ando K, Nakamura H, Nakajima Y
  • Journal Title: Anat Rec(Hoboken) Volume: 295(2) Issue: 2 Pages: 257-67
  • DOI: 10.1002/ar.22400
  • Peer Reviewed
• [Journal Article] Role of sox9 during valvulo-septal endocardial cushion formation (2011)
  • Author(s): Yamagishi T, Yanagawa N, Takahashi M, Ando M, Nakamura H, Nakajima Y
  • Journal Title: J Physiol Sci Volume: 61
• [Journal Article] Immunohistochemical studies of aortic smooth muscle cells in quail embryo (2011)
  • Author(s): Ando K, Nakajima Y, Yamagishi T, Nakamura H
  • Journal Title: J Physiol Sci Volume: 61
• [Presentation] 心臓弁・中隔原基である心内膜床形成過程における上皮-間葉形質転換でのsox9の役割 (2012)
  • Author(s): 山岸敏之, 高橋真樹子, 柳川成章, 甲斐理武, 中島裕司
  • Organizer: 第117回日本解剖学会全国学術総会
  • Place of Presentation: 山梨大学甲府
  • Year and Date: 2012-03-26
• [Presentation] 心内膜床形成過程でおこる上皮-間葉形質転換でのsox9の役割 (2012)
  • Author(s): 山岸敏之
  • Organizer: 解剖学会学術集会
  • Place of Presentation: 山梨大学(甲府)
  • Year and Date: 2012-03-26
• [Presentation] Epicardial TGFβSignal Is Required for Myocardial Maturation Before the Onset of Coronary Circulation (2011)
  • Author(s): Takahashi M, Yanagawa N, Kai M, Yamagishi T, Nakajima Y
  • Place of Presentation: デンバー.アメリカ
  • Year and Date: 2011-12-06
• [Presentation] 心臓弁・心臓中隔原基形成過程における転写因子Msx1の役割 (2011)
  • Author(s): 山岸敏之、中島裕司
  • Place of Presentation: シェーンバッハ砂防.東京
  • Year and Date: 2011-07-23
• [Presentation] 胚心臓における心内膜床形成過程でのsox9の役割 (2011)
  • Author(s): 山岸敏之、柳川成章、高橋真樹子、安藤克己、中村裕昭、中島裕司
  • Organizer: 第88回日本生理学会大会・第116回日本解剖学会全国学術総会合同大会
  • Year and Date: 2011-03-28
• [Presentation] ウズラ胚の大動脈中膜平滑筋細胞に関する免疫組織化学的研究 (2011)
  • Author(s): 安藤克己、中島裕司、山岸敏之、中村裕昭
  • Organizer: 第88回日本生理学会大会・第116回日本解剖学会全国学術総会合同大会
  • Year and Date: 2011-03-28
• [Presentation] Nodal signal is required for morphogenetic movements of epiblast cells pre-streak chick blastderm (2010)
  • Author(s): Yanagawa N, Yamagishi T, Nakajima Y
  • Organizer: Society for Developmental Biology 69th Annual Meeting
  • Place of Presentation: アルバカーキ.アメリカ
  • Year and Date: 2010-08-06
• [Presentation] 心内膜床形成でのsox9の役割:心臓弁・中隔原基形成の分子機構 (2009)
  • Author(s): 山岸敏之, 柳川成章, 寺迫優美, 中村裕昭, 中島裕司
  • Organizer: 第85回日本解剖学会近畿支部学術集会
  • Place of Presentation: 奈良県立医科大学奈良
  • Year and Date: 2009-11-28