Distribution of Intraepithelial lymphocytes in the murine small intestine : The variability of the morphological property and function
| Project/Area Number |
21590207
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
ITOH Tsunetoshi 東北大学, 大学院・医学系研究科, 教授 (90004746)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OGATA Masaki 東北大学, 大学院・医学系研究科, 助教 (50311907)
KASAHARA Shigeru (MATUTANI Takaji) 東北大学, 大学院・医学系研究科, 非常勤講師 (70372290)
笠原 森 東北大学, 大学院・医学系研究科, 非常勤講師 (70161007)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | IEL / 小腸 / マウス / DNA断片化 / 免疫組織化学 / 電子顕微鏡 / Flow Cytometry / 絨毛上皮細胞 / iIEL / グランザイムB / パーフォリン / 抗CD3抗体 / in vivo / Flow Cytometer |
|---|
| Research Abstract |
Different intestinal intraepithelial lymphocytes (IEL)subgroups (presence of granules, etc.)in different villus sites were demonstrated by morphological analysis. Granzyme B (GrB)has been proved to be involved in the induction of DNA fragmentation of epithelial cells due to IEL activation, the results suggest the possibility of a new DNA fragmentation induction mechanism different from that of previously reported perforin (Pfn)/GrB system. We also found that IELs in the front line of the intestinal defense system are highly stimulus-sensitive, thus are disposable, and, when stimulated, finally causing DNA fragmentation in the surrounding villus epithelial cells.
|
Report
(4 results)
Research Products
(23 results)
-
-
-
-
-
-
-
-
[Journal Article] The interaction of monocytes with rheumatoid synovial cells is a key step in LIGHT-mediated inflammatory bone destruction.2009
Author(s)
shida S, Yamane S, Nakano S, Yanagimoto T, Hanamoto Y, Maeda-Tanimura M, Toyosaki-Maeda T, Ishizaki J, Matsuo Y, Fukui N, Itoh T, Ochi T, Suzuki R.
-
Journal Title
Related Report
-
[Journal Article] T cell receptor repertoire in BALB/c mice varies according to tissue type, sex, age, and hydrocortisone treatment.2009
Author(s)
Kitaura K, Kanayama K, Fujii Y, Shiobara N, Tanaka K, Kurane I, Suzuki S, Itoh T, Suzuki R.
-
Journal Title
Experimental Animals.
Volume: 58
Pages: 159-168
NAID
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
