| Project/Area Number |
21590207
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Tohoku University |
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Principal Investigator |
ITOH Tsunetoshi 東北大学, 大学院・医学系研究科, 教授 (90004746)
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| Co-Investigator(Kenkyū-buntansha) |
OGATA Masaki 東北大学, 大学院・医学系研究科, 助教 (50311907)
KASAHARA Shigeru (MATUTANI Takaji) 東北大学, 大学院・医学系研究科, 非常勤講師 (70372290)
笠原 森 東北大学, 大学院・医学系研究科, 非常勤講師 (70161007)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | IEL / 小腸 / マウス / DNA断片化 / 免疫組織化学 / 電子顕微鏡 / Flow Cytometry / 絨毛上皮細胞 / iIEL / グランザイムB / パーフォリン / 抗CD3抗体 / in vivo / Flow Cytometer |
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| Research Abstract |
Different intestinal intraepithelial lymphocytes (IEL)subgroups (presence of granules, etc.)in different villus sites were demonstrated by morphological analysis. Granzyme B (GrB)has been proved to be involved in the induction of DNA fragmentation of epithelial cells due to IEL activation, the results suggest the possibility of a new DNA fragmentation induction mechanism different from that of previously reported perforin (Pfn)/GrB system. We also found that IELs in the front line of the intestinal defense system are highly stimulus-sensitive, thus are disposable, and, when stimulated, finally causing DNA fragmentation in the surrounding villus epithelial cells.
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