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Analysis of microdomain of the apical and basolateral membrane in epithelial cells with polarity

Research Project

Project/Area Number 21590209
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionGunma University

Principal Investigator

AOKI Takeo  群馬大学, 大学院・医学系研究科, 講師 (70150919)

Co-Investigator(Kenkyū-buntansha) HAGIWARA Haruo  帝京大学, 医学部, 教授 (80189464)
Co-Investigator(Renkei-kenkyūsha) MATSUURA Tsutomu  群馬大学, 大学院・工学系研究科, 准教授 (80181692)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords膜微小ドメイン / アクアポリン-2 / リサイクリングシステム / MDCK細胞 / カベオラ / シンタキシン-6 / 初期エンドソーム / 糖脂質合成阻害剤 / アクアポリン2 / シンタキシン6 / カベオリン1 / コレステロール / RNAi / エンドサイトーシス
Research Abstract

In MDCK cells stably expressing human AQP2 (MDCK-hAQP2), AQP2 is trafficked to the apical special membrane by forskolin and then internalized to the intracellular compartment by some mechanisms. The results of experiments in caveolin-1 knockdown cells by RNA interference or ceramide glucosyltransferase inhibitor treated cells, sialidase treated cells suggest that AQP2 expression and its retention in the apical membrane, its destination is closely related with the completeness of membrane microdomain components.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (41 results)

All 2012 2011 2010 2009 Other

All Journal Article (12 results) (of which Peer Reviewed: 5 results) Presentation (28 results) Remarks (1 results)

  • [Journal Article] Close Association of Aquaporin-2 Internalization with Caveolin-12012

    • Author(s)
      Aoki T., 他
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 45 Issue: 2 Pages: 139-146

    • DOI

      10.1267/ahc.12003

    • NAID

      130001854241

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Blind Source Separation of Mixed Images Using 2-D Complex Wavelet Transform2011

    • Author(s)
      Matsuura T, A. Faiz, K. Kiryu
    • Journal Title

      Journal of the Japan Society of Applied Electro-magnetics and Mechanics

      Volume: Vol.19 Pages: 234-239

    • NAID

      110008904263

    • Related Report
      2011 Final Research Report
  • [Journal Article] Myosin light chain kinase/actin interaction in phorbol dibutyrate-stimulated smooth muscle cells2011

    • Author(s)
      Thatcher SE, Fultz ME, Tanaka H, Hagiwara H, Zhang HL, Zhang Y, Hayakawa K, Yoshiyama S, Nakamura A, Wang HH, Katayama T, Watanabe M, Lin Y, Wright GL, Kohama K
    • Journal Title

      J Pharmacol Sci

      Volume: 116 Pages: 116-127

    • NAID

      10029894196

    • Related Report
      2011 Final Research Report
  • [Journal Article] Myosin Light Chain Kinase / Actin Interaction in Phorbol Dibutyrate-Stimulated Smooth Muscle Cells2011

    • Author(s)
      Thatcher S.E., 他
    • Journal Title

      Journal of Pharmacological Sciences

      Volume: 116 Issue: 1 Pages: 116-127

    • DOI

      10.1254/jphs.10296FP

    • NAID

      10029894196

    • ISSN
      1347-8613, 1347-8648
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the medaka (Oryzias latipes) primary ciliary dyskinesia mutant, jaodori: redundant and distinct roles of dynein axonemal intermediate gene 2 (dnai2) in motile cilia2010

    • Author(s)
      Kobayashi D, Iijima N, Hagiwara H, Kamura K, Takeda H, Yokoyama T
    • Journal Title

      Developmental Biology

      Volume: 347 Pages: 62-70

    • Related Report
      2011 Final Research Report
  • [Journal Article] Pre-embedding immunoelectron microscopy of chemically fixed mammalian tissue culture cells2010

    • Author(s)
      Hagiwara H, Aoki T, Suzuki T, Takata K
    • Journal Title

      Schwartzbach SD, Osafune T, eds, Immunoelectron microscopy, methods and protocols

      Pages: 145-154

    • Related Report
      2011 Final Research Report
  • [Journal Article] Double-label immunoelectron microscopy for studying the colocalization of proteins in culture cells2010

    • Author(s)
      Hagiwara H, Aoki T, Suzuki T, Takata K
    • Journal Title

      Schwartzbach SD, Osafune T, eds, Immunoelectron microscopy, methods and protocols

      Pages: 249-257

    • Related Report
      2011 Final Research Report
  • [Journal Article] Double-label immunoelectron microscopy for studying the colocalization of proteins in cultured cells.2010

    • Author(s)
      Hagiwara, et al.
    • Journal Title

      Methods Mol Biol.

      Volume: 657 Pages: 249-257

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pre-embedding immunoelectron microscopy of chemically fixed mammalian tissue culture cells.2010

    • Author(s)
      Hagiwara H., et al.
    • Journal Title

      Methods Mol Biol.

      Volume: 657 Pages: 145-154

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Localization of Inv in a distinctive intraciliary compartment requires the C-terminal ninein-homolog-containing region2009

    • Author(s)
      Shiba D, Yamaoka Y, Hagiwara H, Takamatsu T, Hamada H, Yokoyama T
    • Journal Title

      J. Cell Sci

      Volume: 122 Pages: 44-54

    • Related Report
      2011 Final Research Report
  • [Journal Article] Localization of Inv in a distinctive intraciliary compartment requires the C-terminal ninein-homolog-containing region.2009

    • Author(s)
      Shiba D, et al.
    • Journal Title

      Journal of Cell Science 122(Pt1)

      Pages: 44-54

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Close association of aquaporin-2 internalization with caveolin-1

    • Author(s)
      Aoki T, Suzuki T, Hagiwara H, Kuwahara M, Sasaki S, Takata K, Matsuzaki T
    • Journal Title

      Acta Histochem Cytochem

      Volume: (in press)

    • NAID

      130001854241

    • Related Report
      2011 Final Research Report
  • [Presentation] Morphoogical analysis of effects with arachidonic acid in smooth muscle cells2012

    • Author(s)
      田中秀幸, 他
    • Organizer
      第117回日本解剖学会総会学術集会
    • Place of Presentation
      山梨大学甲府キャンパス
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Morphoogical analysis of effects with arachidonic acid in smooth muscle cells2012

    • Author(s)
      Tanaka H, Hagiwara H, Mitui S
    • Organizer
      第117回日本解剖学会
    • Place of Presentation
      山梨大学甲府キャンパス(山梨県)
    • Related Report
      2011 Final Research Report
  • [Presentation] MDCK細胞頂部におけるAQP2保持能力に関する膜微小ドメイン構成成分に関する検討2011

    • Author(s)
      青木武生, 松崎利行, 高田邦昭
    • Organizer
      第52回日本組織細胞化学会総会・学術集会講演会
    • Place of Presentation
      金沢大学宝町キャンパス(石川県)
    • Related Report
      2011 Final Research Report
  • [Presentation] 崎尚、野島美久SIRPαシグナル経路の遮断は糖尿病性腎症を増悪させる2011

    • Author(s)
      高橋哲史, 廣村桂樹, 浜谷博子, 加藤麻衣, 坂入徹, 櫻井則之, 池内秀和, 前嶋明人, 黒岩卓, 青木武生, 大西浩史, 的崎尚, 野島美久
    • Organizer
      第54回日本腎臓学会学術総会
    • Place of Presentation
      パシフィコ横浜会議センター(神奈川県)
    • Related Report
      2011 Final Research Report
  • [Presentation] 平滑筋細胞におけるスフィンゴ脂質による細胞内微細形態変化2011

    • Author(s)
      田中秀幸, 萩原治夫
    • Organizer
      第116回日本解剖学会
    • Place of Presentation
      パシフィコ横浜会議センター(神奈川県)
    • Related Report
      2011 Final Research Report
  • [Presentation] AQP2 retention in the apical membrane is closely related with membrane micro-domain component2011

    • Author(s)
      Aoki T, Hagiwar H, Takata K, Matsuzaki T
    • Organizer
      第116回日本解剖学会総会・全国学術集会
    • Place of Presentation
      パシフィコ横浜会議センター(神奈川県)
    • Related Report
      2011 Final Research Report
  • [Presentation] MDCK細胞頂部におけるAQP2保持能力に関する膜微小ドメイン構成成分に関する検討2011

    • Author(s)
      青木武生, 他
    • Organizer
      第52回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      金沢大学石引キャンパス
    • Related Report
      2011 Annual Research Report
  • [Presentation] SIRPαシグナル経路の遮断は糖尿病性腎症を増悪させる2011

    • Author(s)
      高橋哲史, 他
    • Organizer
      第54回日本腎臓学会学術集会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2011 Annual Research Report
  • [Presentation] SIRPαは腎糸球体上皮細胞の形態と蛋白尿制御に関与する2011

    • Author(s)
      高橋哲史, 他
    • Organizer
      第58回北関東医学会
    • Place of Presentation
      群馬大学前橋キャンパス
    • Related Report
      2011 Annual Research Report
  • [Presentation] AQP2 retention in the apical membrane is closely related with membrane micro domain component.2011

    • Author(s)
      Aoki T., et al.
    • Organizer
      第116回日本解剖学会総会・全国学術集会
    • Place of Presentation
      横浜(震災の為中止)
    • Related Report
      2010 Annual Research Report
  • [Presentation] SIRP-αは糸球体上皮細胞の形態と蛋白尿制御に関与する2010

    • Author(s)
      高橋哲史, ほか
    • Organizer
      Kidney Frontier 2010
    • Place of Presentation
      東京
    • Year and Date
      2010-12-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] 筋形質膜におけるデンスプラーク領域とカベオラ密集領域2010

    • Author(s)
      田中秀幸, 萩原治夫
    • Organizer
      第42回日本臨床分子形態学会
    • Place of Presentation
      東レ総合研修センター(静岡県)
    • Year and Date
      2010-09-24
    • Related Report
      2011 Final Research Report
  • [Presentation] Nojima Y The role of SIRPα (SHPS-1) in experimental diabetic nephropathy. American Society of Nephrology2010

    • Author(s)
      Takahashi T, Hiromura K, Hamatani H, Tomioka M, Maeshima A, Kuroiwa T, Ohnishi H, Matozaki T, Aoki T
    • Organizer
      43rd Annual Meeting & Scientific Exposition
    • Related Report
      2011 Final Research Report
  • [Presentation] SIRP-αは糸球体上皮細胞の形態と蛋白尿制御に関与する2010

    • Author(s)
      富岡麻衣, 廣村桂樹, 高橋哲史, 前嶋明人, 黒岩卓, 青木武生, 高田邦昭, 大西浩史, 的崎尚, 野島美久
    • Organizer
      第53回日本腎臓学会学術総会
    • Place of Presentation
      神戸国際会議場、神戸ポートピアホテル、神戸国際展示場(兵庫県)
    • Related Report
      2011 Final Research Report
  • [Presentation] The Role of SIRPα in Podocyte Structure and Function2010

    • Author(s)
      Tomioka M, Hiromura K, Takahashi T, Maeshima A, Kuroiwa T, Kaneko Y, Ohnishi H, Matozaki T, Aoki T, Takata K, Nojima Y
    • Organizer
      国際腎臓学会NEXUS京都シンポジウム
    • Place of Presentation
      国立京都国際会館(京都府)
    • Related Report
      2011 Final Research Report
  • [Presentation] 頂部膜剥離法によって作製した試料によるAQP2動態の観察2010

    • Author(s)
      青木武生, 萩原治夫, 鈴木健史, 松崎利行, 高田邦昭
    • Organizer
      第115回日本解剖学会総会・全国学術集会
    • Place of Presentation
      岩手県民会館(岩手県)
    • Related Report
      2011 Final Research Report
  • [Presentation] 一次繊毛内分子領域(inv compartment)へのNphp3-Nek8集積機構解析2010

    • Author(s)
      芝大, 萩原治夫, 横山尚彦
    • Organizer
      第115回日本解剖学会
    • Place of Presentation
      岩手県民会館(岩手県)
    • Related Report
      2011 Final Research Report
  • [Presentation] The Role of SIRPα in Podocyte Structure and Function.2010

    • Author(s)
      Tomioka M., et al.
    • Organizer
      国際腎臓学会NEXUS京都シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2010 Annual Research Report
  • [Presentation] SIRP-αは糸球体上皮細胞の形態と蛋白尿制御に関与する2010

    • Author(s)
      富岡麻衣, ほか
    • Organizer
      第53回日本腎臓学会
    • Place of Presentation
      神戸
    • Related Report
      2010 Annual Research Report
  • [Presentation] The Role of SIRPα (SHPS-1) in Experimental Diabetic Nephropathy.2010

    • Author(s)
      Takahashi T., et al.
    • Organizer
      第43回アメリカ腎臓学会
    • Place of Presentation
      デンバー
    • Related Report
      2010 Annual Research Report
  • [Presentation] 頂部膜剥離法によって作製した試料によるAQP2動態の観察2010

    • Author(s)
      青木武生, その他
    • Organizer
      第115回日本解剖学会総会・全国学術集会
    • Place of Presentation
      盛岡市
    • Related Report
      2009 Annual Research Report
  • [Presentation] SIRP-αは糸球体上皮細胞の形態と蛋白尿制御に関与する2009

    • Author(s)
      富岡麻衣, 廣村桂樹, 高橋哲史, 前嶋明人, 黒岩卓, 青木武生, 高田邦昭, 大西浩史, 的崎尚, 野島美久
    • Organizer
      第10回腎不全病態治療研究会
    • Place of Presentation
      全社協灘尾ホール(東京都)
    • Year and Date
      2009-12-12
    • Related Report
      2011 Final Research Report
  • [Presentation] SIRPαは系球体上皮細胞の形態と蛋白尿制御に関与する2009

    • Author(s)
      富岡麻衣, その他
    • Organizer
      第10回腎不全病態治療研究会
    • Place of Presentation
      東京
    • Year and Date
      2009-12-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] 卵管の形態2009

    • Author(s)
      萩原治夫
    • Organizer
      The 10^<th> Annual Symposium Japanese Society for the Advancement of Women's Imaging
    • Place of Presentation
      淡路夢舞台国際会議場(兵庫県)
    • Year and Date
      2009-09-04
    • Related Report
      2011 Final Research Report
  • [Presentation] SIRPα (SHPS-1) signaling pathway is involved in podocyte structure and function、American Society of Nephrology2009

    • Author(s)
      Tomioka M, Hiromura K, Takahashi T, Maeshima A, Kuroiwa T, Kaneko Y, Ohnishi H, Matozaki T, Aoki T, Takata K, Nojima Y
    • Organizer
      42nd Annual Meeting & Scientific Exposition
    • Place of Presentation
      San Diego Convention Center, San Diego, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] MDCK細胞における頂部細胞膜カベオラと水チャネルアクアポリン2との関係2009

    • Author(s)
      青木武生, 萩原治夫, 鈴木健史, 高田邦昭
    • Organizer
      日本組織細胞化学会第50回学術集会
    • Place of Presentation
      ピアザ淡海 滋賀県立県民交流センター(滋賀県)
    • Related Report
      2011 Final Research Report
  • [Presentation] MDCK細胞における頂部細胞膜カベオラと水チャネルアクアポリン2との関係2009

    • Author(s)
      青木武生, その他
    • Organizer
      日本組織細胞化学会50回総会学術集会
    • Place of Presentation
      大津市
    • Related Report
      2009 Annual Research Report
  • [Presentation] SIRPα(SHPS-1)Signaling Pathway Is Involved in Podocyte Structure and Function2009

    • Author(s)
      Tomioka M, et al.
    • Organizer
      第42回アメリカ腎臓学会
    • Place of Presentation
      サンディゴ(USA)
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://anatcb.dept.med.gunma-u.ac.jp/~anat1his/pub.html

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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