Analysis of microdomain of the apical and basolateral membrane in epithelial cells with polarity
Project/Area Number |
21590209
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General anatomy (including Histology/Embryology)
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Research Institution | Gunma University |
Principal Investigator |
AOKI Takeo 群馬大学, 大学院・医学系研究科, 講師 (70150919)
|
Co-Investigator(Kenkyū-buntansha) |
HAGIWARA Haruo 帝京大学, 医学部, 教授 (80189464)
|
Co-Investigator(Renkei-kenkyūsha) |
MATSUURA Tsutomu 群馬大学, 大学院・工学系研究科, 准教授 (80181692)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 膜微小ドメイン / アクアポリン-2 / リサイクリングシステム / MDCK細胞 / カベオラ / シンタキシン-6 / 初期エンドソーム / 糖脂質合成阻害剤 / アクアポリン2 / シンタキシン6 / カベオリン1 / コレステロール / RNAi / エンドサイトーシス |
Research Abstract |
In MDCK cells stably expressing human AQP2 (MDCK-hAQP2), AQP2 is trafficked to the apical special membrane by forskolin and then internalized to the intracellular compartment by some mechanisms. The results of experiments in caveolin-1 knockdown cells by RNA interference or ceramide glucosyltransferase inhibitor treated cells, sialidase treated cells suggest that AQP2 expression and its retention in the apical membrane, its destination is closely related with the completeness of membrane microdomain components.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Myosin light chain kinase/actin interaction in phorbol dibutyrate-stimulated smooth muscle cells2011
Author(s)
Thatcher SE, Fultz ME, Tanaka H, Hagiwara H, Zhang HL, Zhang Y, Hayakawa K, Yoshiyama S, Nakamura A, Wang HH, Katayama T, Watanabe M, Lin Y, Wright GL, Kohama K
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Journal Title
J Pharmacol Sci
Volume: 116
Pages: 116-127
NAID
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[Presentation] 崎尚、野島美久SIRPαシグナル経路の遮断は糖尿病性腎症を増悪させる2011
Author(s)
高橋哲史, 廣村桂樹, 浜谷博子, 加藤麻衣, 坂入徹, 櫻井則之, 池内秀和, 前嶋明人, 黒岩卓, 青木武生, 大西浩史, 的崎尚, 野島美久
Organizer
第54回日本腎臓学会学術総会
Place of Presentation
パシフィコ横浜会議センター(神奈川県)
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[Presentation] The Role of SIRPα in Podocyte Structure and Function2010
Author(s)
Tomioka M, Hiromura K, Takahashi T, Maeshima A, Kuroiwa T, Kaneko Y, Ohnishi H, Matozaki T, Aoki T, Takata K, Nojima Y
Organizer
国際腎臓学会NEXUS京都シンポジウム
Place of Presentation
国立京都国際会館(京都府)
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[Presentation] SIRPα (SHPS-1) signaling pathway is involved in podocyte structure and function、American Society of Nephrology2009
Author(s)
Tomioka M, Hiromura K, Takahashi T, Maeshima A, Kuroiwa T, Kaneko Y, Ohnishi H, Matozaki T, Aoki T, Takata K, Nojima Y
Organizer
42nd Annual Meeting & Scientific Exposition
Place of Presentation
San Diego Convention Center, San Diego, USA
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