Application of photoactivated adenylyl cyclase to study the mechanisims of neurite formation by controlling temporal and spacial intracellular cAMP level in vitro
| Project/Area Number |
21590211
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
INOUE Akihiro 東京医科歯科大学, 大学院・医歯学総合研究科, 准教授 (80322080)
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| Co-Investigator(Kenkyū-buntansha) |
NAKATA Takao 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (50218004)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 神経突起形成 / cAMP / 細胞内シグナル伝達 / 光活性化 / 光活性化アデニル酸シクラーゼ / エレクトロポレーション / メラノサイト / 神経突起伸展 / アデニル酸シクラーゼ / PAC |
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| Research Abstract |
Potencies of the photo-activated adenylyl cyclases(PAC) from euglena and bacteria for controlling intracellular cAMP concentration in vitro were analyzed biochemically with an cAMP-ELISA as well as cell-biologically with an Epac FRET sensor probe. It was also observed that melanosomes labeled with its associated protein Rab27a fused to fluorescent proteins were immediately moved toward peripheral regions of the B16 melanoma cells in response to an increase of the intracellular cAMP. We have also tried to utilize the PACs to study the morphological development and functions of neural cells in PC12 cells and hippocampal primary culture neurons.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Kinase-dead knock-in mouse reveals an essential role of kinase activity of Ca2+/calmodulin-dependent protein kinase IIalpha in dendritic spine enlargement, long-term potentiation, and learning2009
Author(s)
Yamagata Y, Kobayashi S, Umeda T, Inoue A, Sakagami H, Fukaya M, Watanabe M, Hatanaka N, Totsuka M, Yagi T, Obata K, Imoto K, Yanagawa Y, Manabe T, Okabe S
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Journal Title
Journal of Neuroscience
Volume: Vol.29
Pages: 7607-7618
Related Report
Peer Reviewed
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[Presentation] THE ROLE OF KINASE ACTIVITY OF Ca2+/CALMODULIN-DEPENDENT PROTEIN KINASE II IN HIPPOCAMPAL SYNAPTIC PLASTICITY AND LEARNING2009
Author(s)
Yoko Yamagata, Shizuka Kobayashi, Tatsuya Umeda, Akihiro Inoue, Hiroyuki Sakagami, Masahiro Fukaya, Masahiko Watanabe, Nobuhiko Hatanaka, Masako Totsuka, Takeshi Yagi, Kunihiko Oabata, Keiji Imoto, Yuchio Yanagawa, Toshiya Manabe, Shigeo Okabe
Organizer
The 36th International Congress of Physiological Sciences
Place of Presentation
Kyoto, Japan
Related Report
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