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Receptor tyrosine kinase-mediated development and differentiation of interstitial cells in the gastrointestinal tract

Research Project

Project/Area Number 21590213
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionUniversity of Fukui

Principal Investigator

IINO Satoshi  福井大学, 医学部, 教授 (40242854)

Co-Investigator(Kenkyū-buntansha) HORIGUCHI Kazuhide  福井大学, 医学部, 講師 (20377451)
Co-Investigator(Renkei-kenkyūsha) HORIGUCHI Satomi  福井大学, 医学部, 特命助教 (00595283)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords消化管間質細胞 / カハール介在細胞 / 消化管神経系 / Wミュータントマウス / c-Kit / PDGF受容体α / SK3チャネル / 消化管運動 / 消化管 / 線維芽細胞 / SK3 / 消化管神経 / 間質細胞
Research Abstract

We observed the expression of receptor tyrosine kinase, c-Kit and PDGFRα, that are specific markers of interstitial cells of Cajal and fibroblast-like cells in the murine gastrointestinal tract. c-Kit expression was observed in gastrointestinal tract from mid embryonic stage. W mutant mice that have c-kit mutations showed cell type specific deficiency of interstitial cells of Cajal. PDGFRαexpression in gastrointestinal tract was observed from early embryonic stage in mesoderm cells and in fibroblast-like cells in late embryonic stage.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (49 results)

All 2012 2011 2010 2009

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (33 results)

  • [Journal Article] Interstitial cells of Cajal in the gastrointestinal musculature of Wjic c-kit mutant mice2011

    • Author(s)
      S. Iino, S. Horiguchi, K. Horiguchi
    • Journal Title

      Journal of Smooth Muscle Research

      Volume: 47 Issue: 3/4 Pages: 111-121

    • DOI

      10.1540/jsmr.47.111

    • NAID

      10030498082

    • ISSN
      0916-8737, 1884-8796
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serotonin augments gut pacemaker activity via 5-HT3 receptors2011

    • Author(s)
      Hong-Nian Liu, Susumu Ohya, Yuji Nishizawa, Kenta Sawamura, Satoshi Iino, Mohsin Md Syed, Kazunori Goto, Yuji Imaizumi, Shinsuke Nakayama
    • Journal Title

      PLoS ONE

      Volume: 6 Issue: 9 Pages: e24928-e24928

    • DOI

      10.1371/journal.pone.0024928

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Participation of ventral and dorsal tail muscles in bending movements of rat tail2011

    • Author(s)
      H. Hori, T. Fukutani, H. Nakane, S. Iino, Y. Nojyo
    • Journal Title

      Anat Sci Int

      Volume: 86(4) Issue: 4 Pages: 194-203

    • DOI

      10.1007/s12565-011-0110-1

    • NAID

      10031162246

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Properties of acetylcholine-induced relaxation of smooth muscle isolated from the proximal colon of the guinea-pig2010

    • Author(s)
      Y. Kodama, S. Iino, Y. Shigemasa, H. Suzuki
    • Journal Title

      Journal of Smooth Muscle Research

      Volume: 46 Issue: 4 Pages: 185-200

    • DOI

      10.1540/jsmr.46.185

    • NAID

      130000860973

    • ISSN
      0916-8737, 1884-8796
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Downregulation of msh-like 2(msx2) and neurotrophic tyrosine kinase receptor type 2(ntrk2) in the developmental gut of KIT mutant mice2010

    • Author(s)
      S. Horiguchi, K. Horiguchi, Y. Nojyo, S. Iino
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 396(3) Issue: 3 Pages: 774-9

    • DOI

      10.1016/j.bbrc.2010.05.030

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Downregulation of msh-like 2 (msx2) and neurotrophic tyrosine kinase receptor type 2 (ntrk2) in the developmental gut of KIT mutant mice.2010

    • Author(s)
      Horiguchi S., Horiguchi K., Nojyo Y., lino S.
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 396 Pages: 774-779

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Properties of acetylcholine-induced relaxation of smooth muscle isolated from the proximal colon of the guinea-pig.2010

    • Author(s)
      Kodama Y., lino S., Shigemasa Y., Suzuki H.
    • Journal Title

      J.Smooth Muscle Res.

      Volume: 46 Pages: 185-200

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The expression of soluble guanylate cyclase in the vasculature of rat skeletal muscle2009

    • Author(s)
      T. Fukutani, S. Iino, Y. Nojyo
    • Journal Title

      Archives of Histology and Cytology

      Volume: 72 Issue: 2 Pages: 117-126

    • DOI

      10.1679/aohc.72.117

    • NAID

      130004462730

    • ISSN
      0914-9465, 1349-1717
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical demonstration of c-Kit-negative fibroblast-like cells in murine gastrointestinal musculature2009

    • Author(s)
      S. Iino, Y. Nojyo
    • Journal Title

      Archives of Histology and Cytology

      Volume: 72 Issue: 2 Pages: 107-115

    • DOI

      10.1679/aohc.72.107

    • NAID

      130004462729

    • ISSN
      0914-9465, 1349-1717
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acupuncture modulates mechanical responses of smooth muscle produced by transmural nerve stimulation in gastric antrum of genetically hyperglycemic rats2009

    • Author(s)
      H. Fukuta, M. Koshita, E. Nakamura, . Nakamura, A. Yamada, Y. Kawase, T. Ishigami, Y. Kurono, S. Iino, H. Suzuki
    • Journal Title

      Journal of Smooth Muscle Research

      Volume: 45 Issue: 4 Pages: 167-185

    • DOI

      10.1540/jsmr.45.167

    • NAID

      130000860938

    • ISSN
      0916-8737, 1884-8796
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] c-Kit-negative fibroblast-like cells express platelet-derived growth factor receptorαin the murine gastrointestinal musculature2009

    • Author(s)
      S. Iino, K. Horiguchi, S. Horiguchi, Y. Nojyo
    • Journal Title

      Histochem. Cell Biol

      Volume: 131 Issue: 6 Pages: 691-702

    • DOI

      10.1007/s00418-009-0580-6

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interstitial cells of Cajal contain signaling molecules for transduction of nitrergic stimulation in guinea pig caecum2009

    • Author(s)
      Iino S, Horiguchi K, Nojyo Y, Ward SM, Sanders KM.
    • Journal Title

      Neurogastroenterol. Motil. 21

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] c-Kit-negative fibroblast-like cells express platelet-derived growth factor receptor α in the murine gastrointestinal musculature2009

    • Author(s)
      Iino S., Horiguchi K., Horiguchi S., Nojyo Y.
    • Journal Title

      Histochem. Cell Biol. 131

      Pages: 691-702

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Wsh/Wsh c-Kit mutant mice possess interstitial cells of Cajal in the deep muscular plexus layer of the small intestine2009

    • Author(s)
      Iino S., Horiguchi K., Nojyo Y.
    • Journal Title

      Neurosci.Lett. 459

      Pages: 123-126

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical demonstration of c-Kit-negative fibroblast-like cells in murine gastrointestinal musculature2009

    • Author(s)
      Iino S., Nojyo Y.
    • Journal Title

      Arch.Histol.Cytol. 72

      Pages: 107-115

    • NAID

      10027156749

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acupuncture modulates mechanical responses of smooth muscle produced by excitation of intramural nerves in gastric antrum of genetically hyperglycemic rats2009

    • Author(s)
      Fukuta H., Koshita M., Nakamura E., Nakamura H., Yamada A., Kawase Y., Ishigami T., Kurono Y., Iino S., Suzuki H.
    • Journal Title

      J.Smooth Muslce Res. 45

      Pages: 167-185

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 消化管筋層における線維芽細胞の形態と機能2012

    • Author(s)
      飯野哲,堀口和秀,堀口里美
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本市
    • Year and Date
      2012-03-30
    • Related Report
      2011 Final Research Report
  • [Presentation] Morphological and functional properties of fibroblast-like cells in the gastrointestinal musculature2012

    • Author(s)
      S Iino, K Horiguchi, S Horiguchi
    • Organizer
      第89回日本生理学会総会
    • Place of Presentation
      松本市
    • Year and Date
      2012-03-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] 消化管における線維芽細胞の特性2012

    • Author(s)
      飯野哲,堀口和秀,堀口里美
    • Organizer
      第117回日本解剖学会総会
    • Place of Presentation
      甲府市
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 胎生期マウス消化管における平滑筋マーカーおよびc-KIT受容体型チロシンキナーゼ発現2012

    • Author(s)
      堀口和秀,堀口里美,飯野哲
    • Organizer
      第117回日本解剖学会総会
    • Place of Presentation
      甲府市
    • Year and Date
      2012-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス胎生期消化管におけるc-KIT受容体型チロシンキナーゼ発現の解析2011

    • Author(s)
      堀口和秀,堀口里美,飯野哲
    • Organizer
      第52回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      金沢市
    • Year and Date
      2011-09-24
    • Related Report
      2011 Final Research Report
  • [Presentation] c-Kitを発現しないWshミュータントマウスにおけるカハール介在細胞2011

    • Author(s)
      飯野哲,堀口和秀,堀口里美
    • Organizer
      第52回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      金沢市
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] マウス消化管筋層における線維芽細胞様細胞の組織学的検討2011

    • Author(s)
      堀口和秀,飯野哲
    • Organizer
      第53回日本平滑筋学会総会
    • Place of Presentation
      東京
    • Year and Date
      2011-08-04
    • Related Report
      2011 Final Research Report
  • [Presentation] c-Kit遺伝子異常を持つWミュータントマウスにおける消化管カハール介在細胞2011

    • Author(s)
      飯野哲,堀口和秀,堀口里美
    • Organizer
      第53回日本平滑筋学会総会
    • Place of Presentation
      東京
    • Year and Date
      2011-08-03
    • Related Report
      2011 Final Research Report
  • [Presentation] c-Kit遺伝子異常を持つWミュータントマウスにおける消化管カハール介在細胞2011

    • Author(s)
      飯野哲, 堀口和秀, 堀口里美
    • Organizer
      第53回日本平滑筋学会総会
    • Place of Presentation
      東京都
    • Year and Date
      2011-08-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] ICC and fibroblast-like cells: Interstitial cells in the gastrointestinal musculature2011

    • Author(s)
      S lino, K Horiguchi, S Honig uchi
    • Organizer
      第116回日本解剖学会総会
    • Place of Presentation
      横浜(地震のため書面による発表になった)
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Ultrastructural observation of the fibroblast-like cells in murine gastrointestinal musculature2011

    • Author(s)
      K Horiguchi, S Iino
    • Organizer
      第116回日本解剖学会総会
    • Place of Presentation
      横浜(地震のため書面による発表になった)
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Differential gene expression in interstitial cells of Cajal at developmental stage in murine small intestine2011

    • Author(s)
      S Horiguchi, K Horiguchi, S lino
    • Organizer
      第116回日本解剖学会総会
    • Place of Presentation
      横浜(地震のため書面による発表になった)
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Ultrastructural observation of the fibroblast-like cells in murine gastrointestinal musculature2011

    • Author(s)
      K. Horiguchi, S. Iino
    • Organizer
      第116回日本解剖学会総会
    • Related Report
      2011 Final Research Report
  • [Presentation] Differential gene expression in interstitial cells of Cajal at developmental stage in murine small2011

    • Author(s)
      S. Horiguchi, K. Horiguchi, S. Iino
    • Organizer
      第116回日本解剖学会総会
    • Related Report
      2011 Final Research Report
  • [Presentation] ICC and fibroblast-like cells : Interstitial cells in the gastrointestinal musculature2011

    • Author(s)
      S. Iino, K. Horiguchi, S. Horiguchi
    • Organizer
      第116回日本解剖学会総会
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス小腸縦走筋のコリン作動性神経-平滑筋間の情報伝達におけるカハール細胞の役割2010

    • Author(s)
      棚橋靖行,松山勇人,市村嘉郎,木村佳織,海野年弘,飯野哲,小森成一
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広
    • Year and Date
      2010-09-16
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 胎生期マウスにおける小腸ペースメーカー細胞の発生2010

    • Author(s)
      堀口和秀,堀口里美,飯野哲
    • Organizer
      第51回日本組織細胞化学会総会
    • Place of Presentation
      東京都
    • Year and Date
      2010-09-04
    • Related Report
      2011 Final Research Report
  • [Presentation] c-Kit遺伝子異常を持つWミュータントマウスにおける消化管カハール介在細胞2010

    • Author(s)
      飯野哲,堀口和秀,堀口里美
    • Organizer
      第51回日本組織細胞化学会総会
    • Place of Presentation
      東京都
    • Year and Date
      2010-09-04
    • Related Report
      2011 Final Research Report
  • [Presentation] c-Kit遺伝子異常を持つWミュータントマウスにおける消化管カハール介在細胞、2010

    • Author(s)
      飯野哲, 堀口和秀, 堀口里美
    • Organizer
      第51回日本組織細胞化学会総会
    • Place of Presentation
      東京
    • Year and Date
      2010-09-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] 胎生期マウスにおける小腸ペースメーカー細胞の発生2010

    • Author(s)
      堀口和秀, 堀口里美, 飯野哲
    • Organizer
      第51回日本組織細胞化学会総会
    • Place of Presentation
      東京
    • Year and Date
      2010-09-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Differential screeningによるICC特異遺伝子の探索2010

    • Author(s)
      堀口和秀,堀口里美,飯野哲
    • Organizer
      第115回日本解剖学会総会・学術集会
    • Place of Presentation
      盛岡市
    • Year and Date
      2010-03-30
    • Related Report
      2011 Final Research Report
  • [Presentation] 筋層線維芽細胞の特性とネットワーク構成2010

    • Author(s)
      飯野哲
    • Organizer
      第115回日本解剖学会総会・学術集会
    • Place of Presentation
      盛岡市
    • Year and Date
      2010-03-30
    • Related Report
      2011 Final Research Report
  • [Presentation] Morphological arguments for innervation of ICC and involvement of ICC in neurotransmission2010

    • Author(s)
      飯野哲, 堀口和秀
    • Organizer
      The 6th International Symposium on ICC
    • Place of Presentation
      宮崎
    • Year and Date
      2010-02-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウス消化管筋層における線維芽細胞の免疫化学的特徴2009

    • Author(s)
      飯野哲,堀口和秀,堀口里美,野条良彰
    • Organizer
      第50回日本組織細胞化学会総会
    • Place of Presentation
      大津市
    • Year and Date
      2009-09-27
    • Related Report
      2011 Final Research Report
  • [Presentation] Wミュータントマウスにおける小腸カハール介在細胞の発生2009

    • Author(s)
      飯野哲,堀口和秀,堀口里美,野条良彰
    • Organizer
      第50回日本組織細胞化学会総会
    • Place of Presentation
      大津市
    • Year and Date
      2009-09-27
    • Related Report
      2011 Final Research Report
  • [Presentation] Wミュータントマウスにおける小腸カハール介在細胞の発生2009

    • Author(s)
      飯野哲, 堀口和秀, 堀口里美, 野条良彰
    • Organizer
      第50回日本組織細胞化学会総会
    • Place of Presentation
      滋賀
    • Year and Date
      2009-09-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウス消化管筋層における線維芽細胞の免疫化学的特徴2009

    • Author(s)
      飯野哲, 堀口和秀, 堀口里美, 野条良彰
    • Organizer
      第50回日本組織細胞化学会総会
    • Place of Presentation
      滋賀
    • Year and Date
      2009-09-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Immunohistochemical demonstration of c-Kit-negative fibroblast-like cells in the murine gastrointestinal musculature2009

    • Author(s)
      飯野哲, 堀口和秀, 堀口里美, 野条良彰
    • Organizer
      Post-Genomic Advances in the Physiology of Smooth Muscle Satellite Symposium of the IUPS 2009
    • Place of Presentation
      名古屋
    • Year and Date
      2009-07-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] Immunohistochemical demonstration of c-Kit-negative fibroblast-like cells in the murine gastrointestinal musculature.2009

    • Author(s)
      S. Iino, K. Horiguchi, S. Horiguchi, Y. Nojyo
    • Organizer
      Post-Genomic Advances in the Physiology of Smooth Muscle Satellite Symposium of the IUPS 2009
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-07-22
    • Related Report
      2011 Final Research Report
  • [Presentation] 胎生Wマウスを用いたカハール介在細胞の発生に関する形態学的・分子生物学的解析2009

    • Author(s)
      堀口里美,堀口和秀,野条良彰,飯野哲
    • Organizer
      第51回日本平滑筋学会総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-07-22
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス消化管筋層に分布するc-Kit陰性線維芽細胞の免疫化学的特徴2009

    • Author(s)
      飯野哲,堀口和秀,堀口里美,野条良彰
    • Organizer
      第51回日本平滑筋学会総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-07-22
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス消化管筋層に分布するc-Kit陰性線維芽細胞の免疫化学的特徴2009

    • Author(s)
      飯野哲, 堀口和秀, 堀口里美, 野条良彰
    • Organizer
      第51回日本平滑筋学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-07-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] 胎生Wマウスを用いたカハール介在細胞の発生に関する形態学的・分子生物学的解析2009

    • Author(s)
      堀口里美, 堀口和秀, 野条良彰, 飯野哲
    • Organizer
      第51回日本平滑筋学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-07-22
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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