| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
We describe that the 4. 1G colocalized at SLI with a member of the membrane-associated guanylate kinase(MAGUK) family, membrane protein palmitoylated 6(MPP6), and an immunoglobulin superfamily-cell adhesion molecule, CADM4. In 4. 1G-knockout mice, both MPP6 and CADM4 were mostly localized in cytoplasm near Schwann cell nuclei, indicating abnormal protein transport. SLI shape was altered in aged 4. 1G-knockout mouse nerves compared to that of wild-type mice, indicating functional significance of CADM4 for adhesion. Another immunoprecipitation experiment revealed that MPP6 was interacting with 4. 1G. These findings indicate that the 4. 1G has a specific role in targeting of MPP6 and CADM4 to the SLI, and the novel 4. 1G-MPP6-CADM4 membrane skeletal complex probably maintains the structure of SLI as a reservoir against external mechanical forces.
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