Morphofunctional analyses on the Protein 4. 1-family gene deficient mouse
| Project/Area Number |
21590214
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | University of Yamanashi |
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Principal Investigator |
TERADA Nobuo 山梨大学, 大学院・医学工学総合研究部, 医学研究員 (60293461)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 分子形態学 / プロテイン4. 1 / 膜骨格 / 免疫組織化学 / 凍結技法 / 細胞接着 / 膜骨格蛋 / プロテイン4.1 / シュワン細胞 / 末梢神経系 / シュミット-ランターマン切痕 / 接着分子 / 細胞膜 / 遺伝子欠損マウス / 電子顕微鏡 / 接着 / レプリカ技法 |
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| Research Abstract |
We describe that the 4. 1G colocalized at SLI with a member of the membrane-associated guanylate kinase(MAGUK) family, membrane protein palmitoylated 6(MPP6), and an immunoglobulin superfamily-cell adhesion molecule, CADM4. In 4. 1G-knockout mice, both MPP6 and CADM4 were mostly localized in cytoplasm near Schwann cell nuclei, indicating abnormal protein transport. SLI shape was altered in aged 4. 1G-knockout mouse nerves compared to that of wild-type mice, indicating functional significance of CADM4 for adhesion. Another immunoprecipitation experiment revealed that MPP6 was interacting with 4. 1G. These findings indicate that the 4. 1G has a specific role in targeting of MPP6 and CADM4 to the SLI, and the novel 4. 1G-MPP6-CADM4 membrane skeletal complex probably maintains the structure of SLI as a reservoir against external mechanical forces.
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Report
(4 results)
Research Products
(78 results)
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[Journal Article] The cytoskeletal adapter protein 4. 1G organizes the internodes in peripheral myelinated nerves2012
Author(s)
Ivanovic A, Horresh I, Golan N, Spiegel I, Sabanay H, Frechter S, Ohno S, Terada N, Mobius W, Rosenbluth J, Brose N, Peles E.
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Journal Title
J Cell Biol
Volume: 196(3)
Pages: 337-44
Related Report
Peer Reviewed
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[Journal Article] Dispensable role of protein 4. 1B/DAL-1 in rodent adrenal medulla regarding generation of pheochromocytoma and plasmalemmal locali zation of TSLC12009
Author(s)
Ohno N, Terada N, Komada M, Saitoh S, Costantini F, Pace V, Germann PG, Weber K, Yamakawa H, Ohara O, Ohno S.
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Journal Title
Biochim Biophys Acta
Volume: 1793(3)
Pages: 506-15
Related Report
Peer Reviewed
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