Gonadal development and sex differentiation of the gonad specific SF-1 knockout mouse
| Project/Area Number |
21590219
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Aichi Gakuin University (2011)
Yokohama City University (2009-2010) |
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Principal Investigator |
IKEDA Yayoi 愛知学院大学, 歯学部, 教授 (00202903)
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA Masaaki 東京医科歯科大学, 医歯学総合研究科, 准教授 (20193211)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 細胞・組織 / 発生・分化 / 発現制御 / 細胞 |
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| Research Abstract |
The orphan nuclear receptor steroidogenic factor 1(SF-1) plays key roles in gonadal development. This study investigates the postnatal development of the testes and the ovaries of a gonad-specific SF-1 knockout(KO) mouse, in which SF-1 was specifically inactivated. In the KO testes, we detected abnormalities in the seminiferous tubule morphology, the positive cell number for SF-1 and Sertoli cell markers, apoptosis, and cell proliferation, at postnatal day 14(P14) and P21.The KO ovaries exhibited morphologically abnormal ovarian cord and decreased SF-1 immunoreactivity as early as P0.We further investigated testes and ovaries that were exposed to the endocrine disruptor DES during embryonic development, showing that the DES-exposed ovary exhibited morphological abnormalities accompanied with decreased SF-1 expression, during the first 2 weeks after birth. These results suggest SF-1 is essential for normal formation of the seminiferous tubule and the ovarian cord during postnatal development.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Coactivation of SF-1-mediated transcription of steroidogenic enzymes by Ubc9 and PIAS12011
Author(s)
Suda N, Shibata H, Kurihara I, Ikeda Y, Kobayashi S, Yokota K, Murai-Takeda A, Nakagawa K, Oya M, Murai M, Rainey WE, Saruta T, Itoh H
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Journal Title
Endocrinology
Volume: 152
Pages: 2266-2277
Related Report
Peer Reviewed
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