Structure-function studies of voltage-sensors and cAMP-dependent activation in HCN channels

• Project/Area Number: 21590232
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Kyoto University
• Principal Investigator: ISHII Takahiro 京都大学, 大学院・医学研究科, 研究員 (40303812)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: チャネル / 過分極 / cAMP / HCN / Ih / 電位センサー / 変異体 / ペースメーカー / システイン残基 / 点変異

Research Abstract

HCN channels are widely expressed in various tissues, and play important roles in pacemaker and cell excitability. HCN channels are slowly activated by hyperpolarization and by cAMP. This study elucidated the molecular basis of these characteristics in HCN channels.

Research Products

• [Journal Article] Controlled Release of Matrix Metalloproteinase-1 Plasmid DNA Prevents Left Ventricular Remodeling in Chronic Myocardial Infarction of Rats (2011)
  • Author(s): Xue Lin, Hikari Jo, Takahiro M Ishii, Masatoshi Fujita, Michael Fu, Keiichi Tambara, Masaya Yamamoto, Yasuhiko Tabata, Masashi Komeda, and Satoshi Matsuoka
  • Journal Title: Circulation Journal Volume: 73 Pages: 2315-2321
  • URL: http://dx.doi.org/10.1016/j.bbadis.2011.07.011
  • Peer Reviewed
• [Journal Article] Controlled Release of Matrix Metalloproteinase-1 Plasmid DNA Prevents Left Ventricular Remodeling in Chronic Myocardial Infarction of Rats (2009)
  • Author(s): Xue Lin, Hikari Jo, Takahiro M Ishii, Masatoshi Fujita, Michael Fu, Keiichi Tambara, Masaya Yamamoto, Yasuhiko Tabata, Masashi Komeda, Satoshi Matsuoka
  • Journal Title: Circulation Journal 73 Pages: 2315-2321
  • Peer Reviewed
• [Presentation] アドレナリンβ2受容体の基底活性は、HCNチャネルを介してマウス嗅細胞の自発発火活動を維持する (2012)
  • Author(s): 中島則行、石井孝広、大森治紀
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本
• [Presentation] ラットモデルを用いた本態性振戦の遺伝要因の解明 (2011)
  • Author(s): 庫本高志、大野行弘、今奥琢士、笹征史、石井孝広、芹川忠夫
  • Organizer: 第34回神経科学大会
  • Place of Presentation: 横浜
• [Presentation] HCNチャネルは、マウス嗅細胞の自発発火活動を維持する (2011)
  • Author(s): 中島則行、石井孝広、大森治紀
  • Organizer: 第34回神経科学大会
  • Place of Presentation: 横浜
• [Presentation] マウス嗅神経の自発発火におけるHCNチャネルの役割 (2010)
  • Author(s): 中島則行、石井孝広、大森治紀
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡
• [Presentation] INTERACTION BETWEEN S1 AND S4 IN THE VOLTAGE SENSOR OF HCN CHANNELS (2009)
  • Author(s): Takahiro Ishii, Noriyuki Nakashima, Harunori Ohmori
  • Organizer: The 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto
  • Year and Date: 2009-07-30
• [Presentation] INTERACTION BETWEEN S1AND S4 IN THE VOLTAGE SENSOR OF HCNCHANNELS (2009)
  • Author(s): Takahiro Ishii, Noriyuki Nakashima, and Harunori Ohmori
  • Organizer: The 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: 京都
• [Remarks]
  • URL: http://www.nbiol.med.kyoto-u.ac.jp
• [Remarks]
  • URL: http://www.nbiol.med.kyoto-u.ac.jp
• [Remarks]
  • URL: http://www.nbiol.kyoto-u.ac.jp
• [Remarks]
  • URL: http://www.nbiol.med.kyoto-u.ac.jp