Physiological significance of tight junction cation selectivity for Na-dependent sugar absorption in the small intestine

• Project/Area Number: 21590236
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: University of Shizuoka
• Principal Investigator: SUZUKI Yuichi 静岡県立大学, 食品栄養科学部, 教授 (50091707)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 上皮機能 / 消化吸収 / 能動輸送 / トランスポータ / タイト結合 / 小腸 / 大腸 / グルコース吸収 / クロージン / ナトリウウム / カリウム / ナトリウムホメオスターシス

Research Abstract

Glucose and many other nutrients absorption in the mammalian small intestine are mediated by Na-dependent active transport mechanism. The present results have suggested that Na required for these processes is rapidly supplied from the blood side to the lumen through the tight junction, and claudin-15 play a major role in this pathway.

Research Products

• [Journal Article] Loss of claudin-15, but not claudin-2, causes Na deficiency and glucose malabsorption in mouse small intestine (2011)
  • Author(s): Tamura A, Hayashi H, Imasato M, Yamazaki Y, Hagiwara A, Wada M, Noda T, Watababe M, Suzuki Y. and Tsukita S.
  • Journal Title: Gastroenterology Volume: 140(3) Pages: 913-923
• [Journal Article] Proteinase-activated receptors-1 and 2 induce electrogenic Cl-secretion in the mouse cecum by distinct mechanisms (2010)
  • Author(s): Ikehara O, Hayashi H, Watanabe Y, Yamamoto H, Mochizuki T, Hoshino M, Suzuki Y.
  • Journal Title: Am. J. Physiol Volume: 299
• [Journal Article] Involvement of DNA fragmentation of enterocytes in mucosal injury to a mouse jejunum incubated in Ussing chambers (2009)
  • Author(s): Inagaki-Tachibana, E., Tsukahara, T., Kaji, K., Eguchi, R, Kanazawa, H, Hayashi, H. and Suzuki, Y.
  • Journal Title: Nagoya J Med Sci Volume: 71 Pages: 11-18
  • NAID: 110007020082
• [Presentation] 非吸収性マーカーを用いたマウス腸管におけるイオン(K、Na、Cl吸収部位の検討 (2011)
  • Author(s): 小松雪乃, 林久由, 鈴木裕一
  • Organizer: Hindgut Club Japanシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2011-12-17
• [Presentation] 腸管上皮タイト結合部の陽イオン選択透過性のNa+依存性栄養素吸収における役割 (2011)
  • Author(s): 鈴木裕一、林久由
  • Organizer: 第65回日本栄養・食糧学会大会
  • Place of Presentation: 東京
• [Presentation] Na依存性グルコース吸収におけるタイト結合部のNa透過性の役割 (2010)
  • Author(s): 林久由, 他
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] ナトリウム依存性グルコース吸収における細胞間経路のナトリウム透過性の役割 (2010)
  • Author(s): 林久由、野崎健太、田村淳、月田早智子、鈴木裕一
  • Organizer: 第87回日本生理学大会
  • Place of Presentation: 盛岡
• [Presentation] Na+-依存性グルコース吸収におけるタイト結合部のNa+透過性の役割 (2010)
  • Author(s): 林久由、鈴木裕一、田村淳、月田早智子
  • Organizer: 第83回日本生化学大会
  • Place of Presentation: 神戸
• [Presentation] The sodium absorption associated with active glucose absorption in perfused mouse jejunum in vivo (2009)
  • Author(s): 野崎健太, 他
  • Organizer: IUPS 2009
  • Place of Presentation: Kyoto
  • Year and Date: 2009-07-29
• [Presentation] The sodium absorption associated with active glucose absorption in perfused mouse jejunum in vivo (2009)
  • Author(s): Kenta Nozaki, Hisayoshi Hayashi, Atsushi Tamura, Sachiko Tsukita and Yuichi Suzuki
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: 京都
• [Presentation] 小腸タイト結合部のイオン透過性の生理的役割の検討 (2009)
  • Author(s): 野崎健太、林久由、田村淳、月田早智子、鈴木裕一
  • Organizer: 第56回中部日本生理学会
  • Place of Presentation: 金沢