Isolation and functional expression of novel putative carbon dioxide(CO_2) receptors in respiratory epithelium.

• Project/Area Number: 21590238
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Nagoya City University
• Principal Investigator: UEDA Takashi 名古屋市立大学, 大学院・医学研究科, 准教授 (90244540)
• Co-Investigator(Kenkyū-buntansha): SHIMADA Shoichi 大阪大学, 大学院・医学系研究科, 教授 (20216063) ; UGAWA Shinya 名古屋市立大学, 大学院・医学研究科, 教授 (20326135) ; ISHIDA Yusuke 大阪大学, 大学院・医学系研究科, 講師 (30381809)
• Co-Investigator(Renkei-kenkyūsha): SHIMADA Shoichi 大阪大学, 大学院・医学系研究科, 教授 (20216063) ; ISHIDA Yusuke 大阪大学, 大学院・医学系研究科, 講師 (30381809)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 受容体 / 細胞内シグナル伝達 / 化学感覚 / 化学受容器 / シグナル伝達 / 呼吸器系 / 上皮細胞 / 嗅覚系CO_2受容器 / 嗅覚系CO2受容機 / 嗅覚系CO2受容機構

Research Abstract

Mouse nasal epithelial cells partially exhibited CO_2-induced intracellular calcium mobilization. These cells did not respond to the ligands that activate proton-sensitive cation channels, such as transient receptor potential vanilloid 1(TRPV1), acid-sensing ion channel(ASIC) 1a, ASIC1b, ASIC2a, ASIC2b and ASIC3.Since the nasal epithelial cells responded to NaHCO_3 solution rather than acidic pH solution, a mechanism for detection of HCO_3-may be involved in nasal CO_2 perception.

Research Products

• [Journal Article] Acid inhibits TRPV4-mediated Ca^2+influx in mouse esophageal epithelial cells (2011)
  • Author(s): Shikano M, Ueda T, Kamiya T, Ishida Y, Yamada T, Mizushima T, Shimura T, Mizoshita T, Tanida S, Kataoka H, Shimada S, Ugawa S, Joh T
  • Journal Title: Neurogastroenterol Motil. Volume: 23(11) Issue: 11 Pages: 1020-1028
  • DOI: 10.1111/j.1365-2982.2011.01767.x
  • Peer Reviewed
• [Journal Article] The TRPV4 channel is a novel regulator of intracellular Ca^2+in human esophageal epithelial cells (2011)
  • Author(s): Ueda T, Shikano M, Kamiya T, Joh T, Ugawa S
  • Journal Title: Am J Physiol Gastrointest Liver Physiol. Volume: 301(1) Issue: 1 Pages: 138-147
  • DOI: 10.1152/ajpgi.00511.2010
  • Peer Reviewed
• [Journal Article] Geissoschizine methyl ether has third-generation antipsychotic-like actions at the dopamine and serotonin receptors (2011)
  • Author(s): Ueda T, Ugawa S, Ishida Y, Shimada S
  • Journal Title: Eur J Pharmacol Volume: 671 Issue: 1-3 Pages: 79-86
  • DOI: 10.1016/j.ejphar.2011.09.007
  • Peer Reviewed
• [Journal Article] TRPM8-expressing dorsal root ganglion neurons project dichotomizing axons to both skin and bladder in rats. (2011)
  • Author(s): Shibata Y, Ugawa S, Imura M, Kubota Y, Ueda T, 他
  • Journal Title: Neuroreport Volume: (In press)
  • Peer Reviewed
• [Journal Article] TRPV2 activation induces apoptotic cell death in human T24 bladdercancer cells : A potential therapeutic target for bladder cancer. (2010)
  • Author(s): Yamada T Ueda T, Shibata Y, Ikegami Y, Saito M, 他
  • Journal Title: Urology Volume: 76
  • Peer Reviewed
• [Journal Article] Distinct expression of cold receptors (TRPM8 and TRPA1) in the rat nodose-petrosal ganglion complex. (2010)
  • Author(s): Hondoh A, Ishida Y, Ugawa S, Ueda T, Shibata Y, 他
  • Journal Title: Brain Res. Volume: 1319 Pages: 60-69
  • Peer Reviewed
• [Journal Article] TRPV3, a thermosensitive channel is expressed in mouse distal colon epithelium (2009)
  • Author(s): Ueda T, Yamada T, Ugawa S, Ishida Y, Shimada S.
  • Journal Title: Biochem Biophys Res Commun. 383 Pages: 130-134
  • Peer Reviewed
• [Journal Article] Development of generic calcium imaging assay for monitoring Gi-coupled receptors and G protein interaction (2009)
  • Author(s): Ueda T, Ugawa S, Ishida Y, Hondoh A, Shimada S.
  • Journal Title: J Biomol Screen. 14 Pages: 781-788
  • Peer Reviewed
• [Journal Article] P2X(2)- and P2X(3)-positive fibers in fungiform papillae originate from the chorda tympani but not the trigeminal nerve in rats and mice (2009)
  • Author(s): Ishida Y, Ugawa S, Ueda T, 他
  • Journal Title: J Comp Neurol. 514 Pages: 131-144
  • Peer Reviewed
• [Presentation] ヒト食道上皮細胞における機械刺激とATP放出 (2011)
  • Author(s): 植田高史, 鹿野美千子、北條英ミレナ、渡辺正哉、鵜川眞也
  • Organizer: 第71回日本解剖学会中部支部学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-15
• [Presentation] 消化管上皮におけるthermoTRPチャネルの発現と機能解析(シンポジウム) (2011)
  • Author(s): 植田高史, 鵜川眞也, 石田雄介、島田昌一
  • Organizer: 第116回日本解剖学会総会
  • Place of Presentation: 横浜(予定開催地)
• [Presentation] 食道上皮細胞におけるTRPV4チャネルの発現と機能解析 (2010)
  • Author(s): 植田高史、鹿野美千子、小山智士、渡辺正哉、鵜川眞也
  • Organizer: 第70回日本解剖学会中部支部学術集会
  • Place of Presentation: 岐阜
  • Year and Date: 2010-10-17