The Regulation and Pathophysiology of Intracellular Water Metabolism by an Intracellular Aquaporin

• Project/Area Number: 21590242
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Meiji Pharmaceutical University
• Principal Investigator: ISHIBASHI Kenichi 明治薬科大学, 薬学部, 教授 (80223022)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 水チャネル / 多発性嚢胞腎 / 細胞内 / 小胞体 / 血液脳関門 / ノックアウトマウス / 脳浮腫 / 胸腺 / 浸透圧 / 肝障害 / 腎障害 / 精子形成 / 内皮細胞 / アクアポリン / 脈絡叢 / 敗血症 / 水輸送 / 嚢胞腎 / 細胞内オルガネラ / マイクロアレイ / 2次元蛋白電気泳動

Research Abstract

This study revealed the importance of endoplasmic reticulum(ER) stress reaction induced by the accumulation of unfold proteins in the ER for the development of polycystic kidneys in AQP11 null mice by comprehensive gene and protein expression surveys of the kidney. Other than the kidney, AQP11 plays some roles in the liver, testis, thymus and brain. It is noteworthy that AQP11 is expressed at the endothelium of cerebral capillaries seemingly for the adaptation of osmotic stresses in the brain.

Research Products

• [Journal Article] The evolutionary aspects of aquaporin family (2011)
  • Author(s): Ishibashi K, Kondo S, Hara S, Morishita Y
  • Journal Title: Am J Physiol Regul Integr Comp Physiol Volume: 300 Issue: 3 Pages: R566-R576
  • DOI: 10.1152/ajpregu.90464.2008
  • Peer Reviewed
• [Journal Article] The NPC motif of aquaporin-11, unlike the NPA motif of known aquaporins, is essential for full expression of molecular function (2011)
  • Author(s): Ikeda M, Andoo A, Shimono M, Takamatsu N, Taki A, Muta K, Matsushita W, Uechi T, Matsuzaki T, Kenmochi N, Takata K, Sasaki S, Ito K, Ishibashi K.
  • Journal Title: J Biol Chem Volume: 286 Issue: 5 Pages: 3342-3350
  • DOI: 10.1074/jbc.m110.180968
  • Peer Reviewed
• [Journal Article] Establishment of acute kidney injury mouse model by 0.75% adenine ingestion (2011)
  • Author(s): Morishira, M, et al
  • Journal Title: Ren.Fail. Volume: 33 Pages: 1013-1018
  • Peer Reviewed
• [Journal Article] The evolutionary aspects of aquaporin family (2011)
  • Author(s): Ishibashi, K, et al
  • Journal Title: Am J Physiol Pegul Integr Comp Physiol. Volume: 300
  • Peer Reviewed
• [Journal Article] The interaction of LFA-1 on mononuclear cells and ICAM-1 on tubular epithelial cells accelerates TGF-β1-induced renal epithelial- (2011)
  • Author(s): Morishita, Y, et al
  • Journal Title: PLoS One. Volume: 6 Issue: 8 Pages: e23267-e23267
  • DOI: 10.1371/journal.pone.0023267
  • Peer Reviewed
• [Journal Article] The NPC motif of aquaporin-11, unlike the NPA motif of known aquaporins, is essential for full expression of molecular function. (2011)
  • Author(s): Ikeda M, et al.
  • Journal Title: J Biol Chem Volume: 286 Pages: 3342-3350
  • Peer Reviewed
• [Journal Article] The evolutionary aspects of aquaporin family. (2011)
  • Author(s): Ishibashi K, et al.
  • Journal Title: Am J Physiol Regul Integr Comp Physiol. Volume: 300
  • Peer Reviewed
• [Journal Article] 大学院臨床医学特論講義:腎不全の病態生理からみた薬物療法の進歩 (2010)
  • Author(s): 石橋賢一
  • Journal Title: 明治薬科大学研究紀要 Volume: 39 Pages: 14-18
• [Journal Article] Aquaporin water channels in mammals (2009)
  • Author(s): Ishibashi K, Hara S, Kondo S.
  • Journal Title: Clin Exp Nephrol Volume: 13 Issue: 2 Pages: 107-117
  • DOI: 10.1007/s10157-008-0118-6
  • NAID: 10005661794
  • Peer Reviewed
• [Journal Article] Pancreas- specific aquaporin 12 null mice showed increased susceptibility to caerulein-induced acute pancreatitis (2009)
  • Author(s): Ohta E, et.al
  • Journal Title: Am J Physiol Cell Physiol. 297 Pages: 1368-1378
  • Peer Reviewed
• [Journal Article] New members of mammalian aquaporins : AQP10-AQP12 (2009)
  • Author(s): 石橋賢一
  • Journal Title: Handbook of Experimental Pharmacology 190 Pages: 251-261
  • Peer Reviewed
• [Journal Article] Aquaporin water channels in mammals (2009)
  • Author(s): 石橋賢一, et al.
  • Journal Title: Clin Exp Nephrol 13 Pages: 107-117
  • NAID: 10005661794
  • Peer Reviewed
• [Journal Article] The role of a group III AQP, AQP11 in intracellular organelle homeostasis (2009)
  • Author(s): 石橋賢一, et al.
  • Journal Title: The Journal of Medical Investigation 56 supplement Pages: 312-317
  • NAID: 130004822627
  • Peer Reviewed
• [Journal Article] 水チャネルと体液調節の異常 (2009)
  • Author(s): 石橋賢一
  • Journal Title: Current Therapy 27 Pages: 52-55
• [Journal Article] 大学院特論講義:水電解質研究の進歩 (2009)
  • Author(s): 石橋賢一
  • Journal Title: 明治薬科大学研究紀要 38 Pages: 21-28
• [Presentation] pression in the Brain : Choroid Plexus and Capillaries with Blood-Brain B (2011)
  • Author(s): Koike, S, et al
  • Organizer: American Society of Nephrology
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2011-11-12
• [Presentation] Aquaporin-11 Expression in the Brain : Choroid Plexus and Capillaries with Blood-Brain Barrier Function. Renal Week 2011 (2011)
  • Author(s): Koike S, Tanaka Y, Morishita Y, Ishibashi K
  • Organizer: American Society of Nephrology
  • Place of Presentation: Philadelphia, USA
• [Presentation] Systemic Distribution of AQP11 and Phenotypic Analysis of AQP11-Null Mice (2010)
  • Author(s): Ishibashi K.
  • Organizer: American Society of Nephrology
  • Place of Presentation: Denver, USA
  • Year and Date: 2010-11-20
• [Presentation] Systemic Distribution of AQP11 and Phenotypic Analysis of AQP11-Null Mice. Renal Week 2010 (2010)
  • Author(s): Ishibashi K.
  • Organizer: American Society of Nephrology
  • Place of Presentation: Denver, USA
• [Presentation] Symposium : The physiological roles of subcellular aquaporins (2009)
  • Author(s): 石橋賢一
  • Organizer: 36^<th> International Congress of Physiological Sciences
  • Place of Presentation: Kyoto
  • Year and Date: 2009-07-28
• [Presentation] Symposium : The role of AQP11 in intracellular organelle homeostasis (2009)
  • Author(s): 石橋賢一
  • Organizer: The 11th International Symposium on Exocrine Secretion(ISES)
  • Place of Presentation: Tokushima
  • Year and Date: 2009-07-24
• [Presentation] The role of AQP11 in intracellular organelles (2009)
  • Author(s): Tanaka Y, Hirokawa T, Kondo S, Hara S, Ishibashi K.
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
• [Presentation] Pancreas-specific aquaporin12 null mice showed increased susceptibility to caerulein-induced acute pancreatitis (2009)
  • Author(s): Ohta E
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: Kyoto
• [Presentation] The role of AQP11 in intracellular organelles (2009)
  • Author(s): 田中靖子
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: Kyoto
• [Presentation] Loss of Aquaporin-11 Causes Both the Apoptosis and the Enhanced Cell Proliferation of the Renal Proximal Tubule Cells (2009)
  • Author(s): 小林克樹
  • Organizer: Renal Week2009, American Society of Nephrology
  • Place of Presentation: San Diego, USA
• [Presentation] A NPC Motif of AQP11, Instead of the NPA Motif of Known AQPs, Hasan Important Role in Its Water Permeability (2009)
  • Author(s): Ikeda M
  • Organizer: Renal Week2009, American Society of Nephrology
  • Place of Presentation: San Diego, USA
• [Presentation] 細胞内オルガネラにおけるマウスアクアポリン11 によるイオン環境の制御 (2009)
  • Author(s): 田中靖子
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
• [Presentation] 哺乳類アクアポリン10(AQP10)遺伝子の偽遺伝子化 (2009)
  • Author(s): 近藤信太郎
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
• [Presentation] AQP11ノックアウトマウスにおける primary cilia の解析 (2009)
  • Author(s): 小林克樹
  • Organizer: 第52回日本腎臓学会学術総会
  • Place of Presentation: 横浜
• [Presentation] アクアポリン11による細胞内オルガネラのイオン環境の制御 (2009)
  • Author(s): 石橋賢一
  • Organizer: 第52回日本腎臓学会学術総会
  • Place of Presentation: 横浜
• [Book] 尿路結石 In腎臓内科学(佐々木成編集)pp192-195 (2010)
  • Author(s): 石橋賢一
  • Total Pages: 4
  • Publisher: シュプリンガー・ジャパン
• [Book] Handbook of Experimental Pharmacology 190(In "Aquaporins" Beitz E ed) (2009)
  • Author(s): Ishibashi K
  • Publisher: Springer-Verlag Berlin Heidelberg
• [Remarks]
• [Remarks]
  • URL: http://www.my-pharm.ac.jp/~kishiba/sub3.html
• [Remarks]
  • URL: http://www.my-pharm.ac.jp/~kishiba/sub7.html
• [Remarks]
  • URL: http://www.my-pharm.ac.jp/~kishiba/sub4.html