Analysis of the network for the vocalization-respiration mode switching mechanism

• Project/Area Number: 21590245
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Hyogo Medical University
• Principal Investigator: ARATA Akiko 兵庫医科大学, 医学部, 准教授 (00266082)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 橋結合腕傍核 / 吸息-呼息切り替え機構 / NMDA / GABA / 新生ラット / NMDAレセプター / 随意運動 / 能動的吸息-呼息切り替え / 呼吸性ニューロン

Research Abstract

The nucleus parabrachialis(NPB) is known as a respiratory modulating center and the NPB receives direct inputs from the vocal and insular cortices associated with the higher processing of emotion and speech. We examined how the NPB participates in the inspiratory-expiratory phase switching using brainstem-spinal cord preparations obtained from 0-4-day-old rats. The NPB electrical stimulation induced a transient depression or termination in the C4 inspiratory phase. This inhibition of C4 was greatly reduced by perfusion of NMDA antagonists and was blocked by GABAA-antagonist. We identified inspiratory-expiratory(I-E) neurons that responded vagal stimulation in the NPB. These I-E neurons which might be inspiratory off-switch neurons received the inputs from the para-facial respiratory group and adrenergic modulation. These results suggested that the NPB is involved in the inspiratory-expiratory phase switching in neonatal rat ; NPB might be working as a phase and/or mode switching system.

Research Products

• [Journal Article] 橋の呼吸調節神経機構 (2012)
  • Author(s): 荒田晶子
  • Journal Title: 自律神経(The Autonomic Nervous System) Volume: 49 Pages: 24-32
  • NAID: 10030555499
  • Peer Reviewed
• [Journal Article] 橋の呼吸調節神経機構 (2012)
  • Author(s): 荒田晶子
  • Journal Title: 自律神経 Volume: 49 Pages: 24-32
  • NAID: 10030555499
  • Peer Reviewed
• [Journal Article] Role of nucleus parabrachialis complex in respiratory circuit in brainstem-spinal cord preparation isolated from neonatal rat(新生ラット摘出脳幹-脊髄標本における呼吸性ニューロンネットワークに対する橋結合腕傍核の役割.) (2010)
  • Author(s): Arata A.
  • Journal Title: Reports of Toyoda Physical and Chemical Research Institute Volume: 63 Pages: 141-4
• [Journal Article] Loss of pre-inspiratory neuron synchroneity in mice with DSCAM deficiency (2010)
  • Author(s): Amano K, Fujii M, Arata S, Ogawa M, Yamakawa K, Arata A.
  • Journal Title: Adv Exp Med Biol Volume: 669 Pages: 15-9
  • Peer Reviewed
• [Journal Article] Adrenaline modulates on the respiratory network development (2010)
  • Author(s): Fujii M, Arata A
  • Journal Title: Adv Exp Med Biol Volume: 669 Pages: 25-8
  • Peer Reviewed
• [Journal Article] Ontogeny of Cl-homeostasis in mouse hypoglossal nucleus (2010)
  • Author(s): Okabe A, Arata A, Oku Y, Takayama C, Fukuda A.
  • Journal Title: Adv. Exp. Med. Biol Volume: 669 Pages: 29-31
  • Peer Reviewed
• [Journal Article] Central respiratory failure in a mouse model depends on the genetic background of the host (2010)
  • Author(s): Arata S, Amano K, Yamakawa K, Arata A
  • Journal Title: Adv Exp Med Biol Volume: 669 Pages: 21-4
  • Peer Reviewed
• [Journal Article] Active inspiratory-expiratory phase switching mechanism exists in the neonatal nucleus parabrachialis (2010)
  • Author(s): Arata A, Tanaka I, Fujii M, Ezure K
  • Journal Title: Adv Exp Med Biol Volume: 669 Pages: 135-8
  • Peer Reviewed
• [Journal Article] Possible role of nucleus parabrachialis in autonomic nervous system (2010)
  • Author(s): Arata, A., Kato, F.
  • Journal Title: Nippon Yakurigaku Zasshi. Volume: 135 Pages: 261-262
  • Peer Reviewed
• [Journal Article] Active inspiratory-expiratory phase switching mechanism exists in the neonatal nucleus parabrachialis. (2010)
  • Author(s): Arata A, Tanaka I, Fujii M, Ezure K.
  • Journal Title: Adv Exp Med Biol. Volume: 669 Pages: 135-138
  • Peer Reviewed
• [Journal Article] Adrenaline modulates on the respiratory network development. (2010)
  • Author(s): Fujii M, Arata A.
  • Journal Title: Adv Exp Med Biol. Volume: 669 Pages: 25-28
  • Peer Reviewed
• [Journal Article] Central respiratory failure in a mouse model depends on the genetic background of the host. (2010)
  • Author(s): Arata S, Amano K, Yamakawa K, Arata A.
  • Journal Title: Adv Exp Med Biol. Volume: 669 Pages: 21-24
  • Peer Reviewed
• [Journal Article] Loss of pre-inspiratory neuron synchroneity in mice with DSCAM deficiency. (2010)
  • Author(s): Amano K, Fujii M, Arata S, Ogawa M, Yamakawa K, Arata A.
  • Journal Title: Adv Exp Med Biol. Volume: 669 Pages: 15-19
  • Peer Reviewed
• [Journal Article] Ontogeny of Cl- homeostasis in mouse hypoglossal nucleus. (2010)
  • Author(s): Okabe A, Arata A, Oku Y, Takayama C, Fukuda A.
  • Journal Title: Adv Exp Med Biol. Volume: 669 Pages: 29-31
  • Peer Reviewed
• [Journal Article] Active inspiratory-expiratory phase switching mechanism exists in the neonatal nucleus parabrachialis. (2010)
  • Author(s): Arata A, Tanaka I, Fujii M, Ezure K.
  • Journal Title: Adv Exp Med Biol. 669 Pages: 135-138
  • Peer Reviewed
• [Journal Article] Adrenaline modulates on the respiratory network development. (2010)
  • Author(s): Fujii M, Arata A.
  • Journal Title: Adv Exp Med Biol. 669 Pages: 25-28
  • Peer Reviewed
• [Journal Article] Respiratory activity of the neonatal dorsolateral pons in vitro (2009)
  • Author(s): Arata A
  • Journal Title: Respir Physiol Neurobiol Volume: 168 Pages: 144-152
  • Peer Reviewed
• [Journal Article] DSCAM deficiency causes loss of pre-inspiratory neuron synchroneity and perinatal death (2009)
  • Author(s): Amano K, Fujii M, Arata S, Tojima T, Ogawa M, Morita N, Shimohata A, Furuichi T, Itohara S, Kamiguchi H, Korenberg JR, Arata A, Yamakawa K.
  • Journal Title: J Neurosci Volume: 29 Pages: 2984-96
  • Peer Reviewed
• [Journal Article] Respiratory activity of the neonatal dorsolateral pons in vitro. (2009)
  • Author(s): Arata, A.
  • Journal Title: Respir Physiol Neurobiol. 168 Pages: 144-152
  • Peer Reviewed
• [Presentation] 胎動性活動の胎生期から新生期の移行メカニズム (2012)
  • Author(s): 下村英毅、谷澤隆邦、荒田晶子
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本
  • Year and Date: 2012-03-31
• [Presentation] 低酸素状態における呼吸リズム調節の発達による変遷 (2012)
  • Author(s): 志賀真理、久米叙子、下村英毅、岡部明仁谷澤隆邦、荒田晶子
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本
  • Year and Date: 2012-03-31
• [Presentation] 低酸素状態における新生児期の呼吸リズム (2011)
  • Author(s): 志賀真理、下村英毅、谷澤隆邦、荒田晶子
  • Organizer: 第39回自律神経研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-12-04
• [Presentation] Adrenergic modulation on the respiratory phase-switching neurons in nucleus parabrachialis complex in the neonatal rat (2011)
  • Author(s): Akiko Arata, Morimitsu Fujii, Ikuko Tanaka, Kazuhisa Ezure
  • Organizer: Neuroscience2011 : 41st annual meeting of Neuroscience
  • Place of Presentation: Washington D. C.
  • Year and Date: 2011-11-13
• [Presentation] Adrenergic modulation on the respiratory phase-switching neurons in nucleus parabrachialis complex in the neonatal rat (2011)
  • Author(s): Arata A, Fujii M, Tanaka I, Ezure K
  • Organizer: Society for Neuroscience 2011 (Neuro2011)
  • Place of Presentation: Washington D.C.
  • Year and Date: 2011-11-13
• [Presentation] 橋結合腕傍核における呼吸性ニューロンの特性 (2011)
  • Author(s): 荒田晶子、下村英毅、藤井盛光、谷澤隆邦
  • Organizer: 第64回日本自律神経学会
  • Place of Presentation: 秋田
  • Year and Date: 2011-10-28
• [Presentation] The differences of the respiratory network between C57BL/6 and Balb/c in Dscam KO mice (2011)
  • Author(s): Akiko Arata, Hideki Shimomura, Kenji Amano, Satoru Arata, Kazuhiro Yamakawa, Takakuni Tanizawa
  • Organizer: The 34^<th> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Presentation] Cyanide modulates on the cervical activity in neonatal rat brainstem-spinal cord preparation (2011)
  • Author(s): Mari Ito-Shiga, Nobuko Kanbara-Kume, Hideki Shimomura, Takakuni Tanizawa, Akiko Arata
  • Organizer: The 34^<th> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Presentation] The differences of the respiratory network between C57BL/6 and Balb/c in Dscam KO mice (2011)
  • Author(s): Arata A, Shimomura H, Amano K, Arata S, Yamakawa K, Tanizawa T
  • Organizer: 第34回日本神経科学学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Presentation] Cyanide modulates on the cervical activity in neonatal rat brainstem-spinal cord preparation (2011)
  • Author(s): Shiga M, Kanbara-Kume N, Shimomura H, Tanizawa T, Arata A
  • Organizer: 第34回日本神経科学学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Presentation] Postnatal changes in Cl-homeostasis related with respiration-related rhythmic activity in mouse hypoglossal nucleus (2011)
  • Author(s): Okabe A, Arata A, Shimizu-Okabe C, Takayama C, Konishi S, Fukuda A
  • Organizer: 第34回日本神経科学学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-15
• [Presentation] Analysis of the respiratory rhythm genesis using knock-out mice with respiratory failure (2011)
  • Author(s): Akiko Arata
  • Organizer: Experimental Biology 2011
  • Place of Presentation: Washington D. C.
  • Year and Date: 2011-04-12
• [Presentation] Analysis of the respiratory rhythm genesis using knock-out mice with respiratory failure (2011)
  • Author(s): Arata A
  • Organizer: Experimental Biology Annual meetings (EB2011)
  • Place of Presentation: Washington D.C.(招待講演)
  • Year and Date: 2011-04-12
• [Presentation] Effects of sodium cyanide on cervical root activity in brainstem-spinal cord preparation (2011)
  • Author(s): Mari Ito-Shiga, Nobuko Kanbara-Kume, Akiko Arata
  • Organizer: 第88回日本生理学会大会・第116回日本解剖学会総会全国学術集会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-29
• [Presentation] The structure and function of the respiratory center viewing from gene knock-out mice(シンポジウム) (2011)
  • Author(s): Akiko Arata
  • Organizer: 第88回日本生理学会大会・第116回日本解剖学会総会全国学術集会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-28
• [Presentation] 小脳神経回路発達における呼吸活動の役割 (2010)
  • Author(s): 荒田晶子, 藤井盛光, 伊藤正男
  • Organizer: 第38回自律神経生理研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-12-03
• [Presentation] Electrophysiological analysis of the spinal network function regarding locomotor-like activity in the in situ adult interferon regulatory factor 8 (IRF8) knockout mice. (2010)
  • Author(s): Itaru Yazawa, Ryusuke Yoshimi, Daniel Kim, Tomohiko Kanno, Akiko Arata, Michael O'Donovan, Keiko Ozato
  • Organizer: Neuroscience2010 : the 40th annual meeting of Neuroscience
  • Place of Presentation: San Diego
  • Year and Date: 2010-11-17
• [Presentation] Electrophysiological analysis of the spinal network function regarding locomotor-like activity in the in situ adult interferon regulatory factor 8(IRF8) knockout mice (2010)
  • Author(s): Itaru Yazawa, Ryusuke Yoshimi, Daniel Kim, Tomohiko Kanno, Akiko Arata, Michael O' Donovan, Keiko Ozato
  • Organizer: Neuroscience2010 : 40th annual meeting of Neuroscience
  • Place of Presentation: サンディエゴ
  • Year and Date: 2010-11-15
• [Presentation] Nucleus Parabrachialis(NPB) gate mechanism : Coordination between the information of speech and respiration US-JAPAN Brain Research Cooperative Program Workshop (2010)
  • Author(s): Akiko Arata
  • Organizer: The NeuroPhysiome : Bridging computational neuroscience and systems biology
  • Place of Presentation: 沖縄
  • Year and Date: 2010-11-07
• [Presentation] 発声-呼吸切り替えにかかわる橋結合腕傍核の神経機構 (2010)
  • Author(s): 荒田晶子, 藤井盛光
  • Organizer: 第63回日本自律神経学会
  • Place of Presentation: 横浜
  • Year and Date: 2010-10-22
• [Presentation] Nucleus Parabrachialis (NPB) gate mechanism : Coordination between the information of speech and respiration. (2010)
  • Author(s): Akiko Arata
  • Organizer: US-JAPAN Brain Research Cooperative Program Workshop The NeuroPhysiome : Bridging computational neuroscience and systems biology.
  • Place of Presentation: 沖縄(招待講演)
  • Year and Date: 2010-10-05
• [Presentation] Correlated expression of the neuron-specific potassium chloride cotransporter 2(KCC2) in the neonatal cerebellum and inferior olive (2010)
  • Author(s): Akiko Arata, Akihito Okabe, Chitoshi Takayama, Atsuo Fukuda, Masao Ito
  • Organizer: Neuro2010(Joint Conference of The 33rd annual Meeting of the Japan Neuroscience Society, The 53rd Annual Meeting of Japanese Society for Neurochemistry and The 20th Annual Meeting of Japanese Neural Network Society.)
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-17
• [Presentation] Hypothalamic orexinergic neurons and flocculus Purkinje cells regulate cardiovascular defense reactions Neuro2010 (2010)
  • Author(s): Naoko Nisimaru, Akiko Arata, Masao Ito
  • Organizer: Joint Conference of The 33rd annual Meeting of the Japan Neuroscience Society, The 53rd Annual Meeting of Japanese Society for Neurochemistry and The 20th Annual Meeting of Japanese Neural Network Society
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-17
• [Presentation] Correlated expression of the neuron-specific potassium chloride cotransporter 2 (KCC2) in the neonatal cerebellum and inferior olive. (2010)
  • Author(s): Akiko Arata, Akihito Okabe, Chitoshi Takayama, Atsuo Fukuda, Masao Ito
  • Organizer: Neuro2010 : (Joint Conference of The 33rd annual Meeting of the Japan Neuroscience Society, The 53rd Annual Meeting of Japanese Society for Neurochemistry and The 20th Annual Meeting of Japanese Neural Network Society.)
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-03
• [Presentation] Hypothalamic orexinergic neurons and flocculus Purkinje cells regulate cardiovascular defense reactions. (2010)
  • Author(s): Naoko Nisimaru, Akiko Arata, Masao Ito
  • Organizer: Neuro2010 : (Joint Conference of The 33rd annual Meeting of the Japan Neuroscience Society, The 53rd Annual Meeting of Japanese Society for Neurochemistry and The 20th Annual Meeting of Japanese Neural Network Society.)
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] Activation of distinct neural ensemble in zebrafish telencephalon following the go/no-go rule change in the goal directed active avoidance learning. (2010)
  • Author(s): Tazu Aoki, Ryo Aoki, Masakazu Agetsuma, Hidenori Aizawa, Akiko Arata, Shin-ichi Higashijima, Hitoshi Okamoto
  • Organizer: Neuro2010 : (Joint Conference of The 33rd annual Meeting of the Japan Neuroscience Society, The 53rd Annual Meeting of Japanese Society for Neurochemistry and The 20th Annual Meeting of Japanese Neural Network Society.)
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] Adrenergic modulation on the pontine respiratory neurons in nucleus parabrachialis complex in the neonatal rat (2010)
  • Author(s): Akiko Arata, Morimitsu Fujii, Junichi Nakai
  • Organizer: The 87th Annual Meeting for the Physiological Society of Japan
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-20
• [Presentation] Adrenergic modulation on the pontine respiratory neurons in nucleus parabrachialis complex in the neonatal rat. (2010)
  • Author(s): Akiko Arata, Morimitsu Fujii, Junichi Nakai
  • Organizer: The 87th Annual Meeting for the Physiological Society of Japan
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-20
• [Presentation] 新生マウス呼吸馴化における低酸素および高二酸化炭素環境に対する応答性 (2010)
  • Author(s): 荒田悟、中町智哉、鬼丸洋、荒田晶子、塩田清二
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-05-12
• [Presentation] Optical imaging of neural activity of zebrafish telencephalon in the goal directed behavior. (2009)
  • Author(s): T.Aoki, M.Agetsuma, H.Aizawa, A.Arata, S.Higaashijima, H.Okamoto
  • Organizer: the 32nd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-18
• [Presentation] Development of rhythmic activity in fetal mice lacking GAD65/67 and VGAT (2009)
  • Author(s): A, Arata, M.Fujii, Y.Yanagawa, K.Obata
  • Organizer: the 32nd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] Respiratory activity involved in the cerebellart circuit formation (2009)
  • Author(s): A.Arata, M.Fujii, M.Ito
  • Organizer: International Union Physiological Science(IUPS 2009)
  • Place of Presentation: 京都
  • Year and Date: 2009-07-30
• [Presentation] Neuronal mechanisms of respiratory modulation. (2009)
  • Author(s): A.Arata, M.Fujii
  • Organizer: International Union Physiological Science(IUPS 2009 Symposium,)
  • Place of Presentation: 京都
  • Year and Date: 2009-07-27
• [Presentation] Occurrence of central respiratory failure in mouse model depend on host genetic background (2009)
  • Author(s): Arata S, Amano K, Yamakawa K, Arata A.
  • Organizer: (Poster presentation) 11th Oxford Conference2009
  • Place of Presentation: 奈良
  • Year and Date: 2009-07-25
• [Presentation] Active inspiratory-expiratory phase switching mechanism in the neonatal nucleus Parabrachialis (2009)
  • Author(s): A.Arata, I.Tanaka, M.Fujii, K.Ezure
  • Organizer: 11^<th> Oxford Conference 2009
  • Place of Presentation: 奈良
  • Year and Date: 2009-07-25
• [Presentation] DSCAM deficiency causes loss of pre-inspiratory neuron synchroneity and perinatal death (2009)
  • Author(s): Amano K, Fujii M, Arata S, Ogawa M, Yamakawa K, Arata A.
  • Organizer: (Symposium speaker) 11th Oxford Conference 2009
  • Place of Presentation: 奈良
  • Year and Date: 2009-07-24
• [Remarks] 荒田晶子、Erik De Schutter Bridging computational neuroscience and system biology日米科学技術協力事業「脳研究」分野研究情報交換セミナー成果報告日米科学技術協力事業「脳研究」分野生理学研究所2011年12月7日、岡崎