Molecular mechanism for the interaction between estrogen receptor and G protein-coupled receptor

• Project/Area Number: 21590254
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: University of Yamanashi
• Principal Investigator: ARITA Jun 山梨大学, 大学院・医学工学総合研究部, 教授 (80128587)
• Co-Investigator(Renkei-kenkyūsha): ISHIDA Maho 山梨大学, 大学院・医学工学総合研究部, 助教 (80362086) ; MITSUI Tetuo 山梨大学, 大学院・医学工学総合研究部, 助教 (20402084)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: エストロジェン / エストロジェン受容体 / エストロジェン反応性配列 / ドーパミン受容体 / ブロモクリプチン / プロラクチン産生細胞 / ERE / プロモクリプチン

Research Abstract

It remains unknown how the interaction by G protein-coupled receptor is involved in the functional regulation of estrogen receptor. We showed that hypothalamic dopamine inhibited transcriptional activation of estrogen receptor through G protein-coupled dopamine receptor in pituitary lactotrophs in culture. The results suggest a novel mechanism of the interaction between nuclear receptors and cell membrane receptors.

Research Products

• [Journal Article] Fetal exposure to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin(TCDD) induces expression of the chemokine genes Cxcl4 and Cxcl7 in the perinatal mouse brain (2011)
  • Author(s): Mitsui, T., Taniguchi, N., Kawasaki, N., Kagami, Y., Arita, J.
  • Journal Title: Journal of Applied Toxicology Volume: 31 Pages: 279-284
  • Peer Reviewed
• [Journal Article] cAMP response element-binding protein(CREB) is required for epidermal growth factor(EGF)-induced cell proliferation and serum response element activation in neural stem cells isolated from the forebrain subventricular zone of adult mice (2011)
  • Author(s): Iguchi, H., Mitsui, T., Ishida, M., Kanba, S., Arita, J.
  • Journal Title: Endocrine Journal Volume: 58 Pages: 747-759
  • NAID: 10029862663
  • Peer Reviewed
• [Journal Article] Inhibition of Bcl3 gene expression mediates the anti-proliferative action of estrogen in pituitary lactotrophs in primary culture (2011)
  • Author(s): Mitsui, T., Ishida, M., Izawa, M., Kagami, Y., Arita, J.
  • Journal Title: Molecular & Cellular Endocrinology Volume: 345 Pages: 68-78
  • Peer Reviewed
• [Journal Article] Inhibition of estrogen receptor transcriptional activity by dopamine D2 receptor activation in pituitary lactotrophs (2011)
  • Author(s): Ishida, M., Arita, J.
  • Journal Title: Joural of Physiological Sciences Volume: 61
• [Journal Article] Inhibition of Bcl3 gene expression mediates the anti-proliferative action of estrogen in pituitary lactotrophs in primary culture (2011)
  • Author(s): Mitsui Tetsuo
  • Journal Title: Molecular and Cellualr Endocrinology Volume: 345 Issue: 1-2 Pages: 68-78
  • DOI: 10.1016/j.mce.2011.07.021
  • Peer Reviewed
• [Journal Article] cAMP response element-binding protein (CREB) is required for epidermal growth factor (EGF)-induced cell proliferation and serum response element activation in neural stem cells isolated from the forebrain subventricular zone of adult mice (2011)
  • Author(s): Iguchi Hironobu
  • Journal Title: Endocrine Journal Volume: 58 Pages: 747-759
  • Peer Reviewed
• [Journal Article] Fetal exposure to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin(TCDD)induces expression of the chemokine genes Cxc14 and Cxc17 in the perinatal mouse brain (2011)
  • Author(s): Mitsui Tetsuo
  • Journal Title: Journal of Applied Toxicology Volume: 31(in press)
  • Peer Reviewed
• [Journal Article] Inhibition of estrogen receptor transcriptional activity by dopamine D2 receDtor activation in pituitary lactotrophs (2011)
  • Author(s): Ishida Maho
  • Journal Title: Joural of Physiological sciences Volume: 61 Pages: 223-223
• [Journal Article] Absence of ligand-independent transcriptional activation of the estrogen receptor via the estrogen response element in pituitary lactotrophs in primary culture (2010)
  • Author(s): Ishida, M., Mitsui T., Izawa M., Arita, J.
  • Journal Title: Journal of Steroid Biochemistry and Molecular Biology Volume: 118 Pages: 93-101
  • Peer Reviewed
• [Journal Article] Absence of ligand-independent transcriptional activation of the estrogen receptor via the estrogen response element in pituitary lactotrophs in primary culture (2010)
  • Author(s): Maho Ishida
  • Journal Title: Journal of Steroid Biochemistry & Molecular Biology Vol.118 Pages: 93-101
  • Peer Reviewed
• [Journal Article] The cAMP response element-binding pro-tein in the bed nucleus of the stria terminalis modulates the formalin-induced pain behavior in the female rat (2009)
  • Author(s): Hagihara, H., Ishida, M., Arita, J., Mitsushima, D., Takahashi, T., Kimura, F., Funabashi, T.
  • Journal Title: European Journal of Neuroscience Volume: 30 Pages: 2379-2386
  • Peer Reviewed
• [Journal Article] The cAMP response element-binding protein in the bed nucleus of the stria terminalis modulates the formalin-induced pain behavior in the female rat (2009)
  • Author(s): Hiroko Hagiwara
  • Journal Title: European Journal of Neuroscience Vol.30 Pages: 2379-2386
  • Peer Reviewed
• [Presentation] プロラクチン産生細胞の細胞増殖に対するエストロジェンの抑制作用の発現機構 (2012)
  • Author(s): 三井哲雄
  • Organizer: 第89回日本生理学会大会「下垂体の機能調節に関わる新規分子の解明」シンポジウム
  • Place of Presentation: (長野県松本市)
  • Year and Date: 2012-03-30
• [Presentation] 下垂体プロラクチン細胞におけるドーパミンD2受容体活性化によるエストロジェン受容体転写活性の抑制 (2011)
  • Author(s): 石田真帆、有田順
  • Organizer: 第88回日本生理学会大会第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: 神奈川県
  • Year and Date: 2011-03-29
• [Presentation] プロラクチン産生細胞の増殖制御におけるエストロジェン作用 (2009)
  • Author(s): 石田真帆、有田順
  • Organizer: 第82回日本内分泌学会
  • Place of Presentation: 群馬県
  • Year and Date: 2009-04-24
• [Presentation] プロラクチン産生細胞の増殖制御におけるエストロジェン作用 (2009)
  • Author(s): 石田真帆
  • Organizer: 第82回 日本内分泌学会
  • Place of Presentation: 群馬県民会館(群馬県)
  • Year and Date: 2009-04-24
• [Remarks]
  • URL: http://www.med.yamanashi.ac.jp/basic/physio01/
• [Remarks]
  • URL: http://www.med.yamanashi.ac.jp/basic/physio01/
• [Remarks]
  • URL: http://www.med.yamanashi.ac.jp/basic/physio01/
• [Remarks]
  • URL: http://www.med.yamanashi.ac.jp/basic/physio01/