Study on the neural network of feeding regulation by selective ablation of histamine H1 receptor-expressing neurons
| Project/Area Number |
21590258
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | The University of Tokushima |
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Principal Investigator |
HORIO Shuhei 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (80145010)
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| Co-Investigator(Kenkyū-buntansha) |
UEYAMA Takashi 和歌山県立医科大学, 医学部, 准教授 (50264875)
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| Co-Investigator(Renkei-kenkyūsha) |
KOBAYASHI Kazuto 福島県立医科大学, 医学部, 教授 (90211903)
MINOKOSHI Yasuhiko 自然科学研究機構, 生理学研究所, 教授 (10200099)
SEI Yoshihiro 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (40206602)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 視床下部 / 室傍核 / 腹内側核 / 摂食調節 / 糖尿病 / ヒスタミン受容体 / イムノトキシン / 神経回路 |
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| Research Abstract |
To identify the neurons involved in the regulation of food intake, we employed highly selective method that ablated specific type of neurons. We have chosen histamine H1 receptor(H1R)-expressing neurons as the target. H1Rs were highly expressed in the paraventricular nucleus of the hypothalamus(PVH). To ablate these neurons, we have constructed gene-targeted(knock-in) mice that express human IL-2R. under the control of the H1R gene. Microinjection of the immunotoxins into PVH selectively ablated H1R-expressing neurons in the mutant mice. The ablation of these neurons increased food intake and body weight gain of the mice. These results indicate that H1R-expressing neurons in the PVH are involved in the regulation of food intake.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Selective ablation of histamine H1 receptor-expressing neurons in the paraventricular nucleus of the hypothalamus to study their role in the regulation of food intake2011
Author(s)
Horio S, Kitaike S, Shiuchi T, Minokoshi Y, Sanbo M, Hirabayashi T, Yagi T, Kai N, Kobayashi K, Ueyama T, Fukui H
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Journal Title
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Related Report
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[Journal Article] Selective ablation of histamine H1 receptor-expressing neurons in the hypothalamus to study their role in the regulation of food intake2011
Author(s)
Horio S, Kitaike S, Shiuchi T, Minokoshi Y, Sanbo M, Hirabayashi T, Yagi T, Kai N, Kobayashi K, Ueyama T, Fukui H
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Journal Title
Related Report
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