| Project/Area Number |
21590258
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | The University of Tokushima |
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Principal Investigator |
HORIO Shuhei 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (80145010)
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| Co-Investigator(Kenkyū-buntansha) |
UEYAMA Takashi 和歌山県立医科大学, 医学部, 准教授 (50264875)
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| Co-Investigator(Renkei-kenkyūsha) |
KOBAYASHI Kazuto 福島県立医科大学, 医学部, 教授 (90211903)
MINOKOSHI Yasuhiko 自然科学研究機構, 生理学研究所, 教授 (10200099)
SEI Yoshihiro 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (40206602)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 視床下部 / 室傍核 / 腹内側核 / 摂食調節 / 糖尿病 / ヒスタミン受容体 / イムノトキシン / 神経回路 |
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| Research Abstract |
To identify the neurons involved in the regulation of food intake, we employed highly selective method that ablated specific type of neurons. We have chosen histamine H1 receptor(H1R)-expressing neurons as the target. H1Rs were highly expressed in the paraventricular nucleus of the hypothalamus(PVH). To ablate these neurons, we have constructed gene-targeted(knock-in) mice that express human IL-2R. under the control of the H1R gene. Microinjection of the immunotoxins into PVH selectively ablated H1R-expressing neurons in the mutant mice. The ablation of these neurons increased food intake and body weight gain of the mice. These results indicate that H1R-expressing neurons in the PVH are involved in the regulation of food intake.
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