Identification of novel immune regulatory factors in the gut immune system and elucidation of molecular mechanisms for their function

• Project/Area Number: 21590260
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Teikyo University
• Principal Investigator: KISHIMOTO Seishi 帝京大学, 薬学部, 教授 (60234217)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 栄養生理学 / 乳 / カゼイン / 腸管免疫 / 生理活性ペプチド / 遊走 / 細胞内カルシウム応答 / 牛乳

Research Abstract

We found that bovine milk casein and its digest with trypsin evoked intracellular calcium mobilization in the macrophage-like cell line through the Gi protein. A novel peptide sequence activating blood cells was identified fromαs1-casein. It was indicated that the unknown receptor coupled with Gi protein was involved in the intracellular calcium response to the peptide. Moreover, the peptide could activate the immune cells selectively.

Research Products

• [Journal Article] Lovastatin alters TGF-β-induced epithelial-mesenchymal transition in porcine lens epithelial cells (2012)
  • Author(s): Urakami, C., Kurosaka, D., Tamada, K., Kishimoto, S., Tezuka, Y. and Nishigori, H.
  • Journal Title: Curr. Eye Res. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Effect of vitamin C depletion on UVR-B induced cataract in SMP30/GNL knockout mice (2012)
  • Author(s): Ishikawa, Y., Hashizume, K., Kishimoto, S. Tezuka, Y., Nishigori, H., Yamamoto, N., Kondo, Y., Maruyama, N., Ishigami, A. and Kurosaka, D.
  • Journal Title: Exp. Eye Res. Volume: Vol.94 Pages: 85-89
  • Peer Reviewed
• [Journal Article] Effect of vitaim C depletion on UVR-B induced cataract in SMP30/GNL knockout mice (2012)
  • Author(s): Yohei Ishikawa, et al
  • Journal Title: Exp.Eye Res. Volume: 94 Issue: 1 Pages: 85-89
  • DOI: 10.1016/j.exer.2011.11.010
  • Peer Reviewed
• [Journal Article] Lovastatin alters TGF-β-induced epithelial-mesenchymal transition in porcine lens epithelial cells (2012)
  • Author(s): Chikako Urakami, et al
  • Journal Title: Curr.Eye Res. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Double-Blind Study on Effects of Glucosyl Ceramide in Beet Extract on Skin Elasticity and Fibronectin Production in Human Dermal Fibroblasts (2010)
  • Author(s): Hori, M., Kishimoto, S., Tezuka, Y. Nishigori, H., Nomoto, K., Hamada, U. and Yonei, Y.
  • Journal Title: Anti-Aging Med. Volume: Vol.7 Pages: 129-142
  • NAID: 130000428432
  • Peer Reviewed
• [Journal Article] Effects of Betamethasone and Mifepristone(RU486) on Brain Acetylcholinesterase of Developing Chick Embryos (2010)
  • Author(s): Yamate, S., Nishigori, H., Kishimoto, S., Tezuka, Y., Fukushima, A. and Nishigori, H.
  • Journal Title: J. Obstet. Gynaecol. Res. Volume: Vol.36 Pages: 11-18
  • Peer Reviewed
• [Journal Article] Double-Blind Study on Effects of Glucosyl Ceramide in Beet Extract on Skin Elasticity and Fibronectin Production in Human Dermal Fibroblasts (2010)
  • Author(s): Mio Hori, et al.
  • Journal Title: Anti-Aging Med. Volume: 7 Pages: 129-142
  • Peer Reviewed
• [Journal Article] Effects of glucocorticoid on brain acetylcholinesterase of developing chick embryos. (2010)
  • Author(s): Sumire Yamate, et al.
  • Journal Title: J.Obstet. Gynaecol.Res. 36 Pages: 11-18
  • Peer Reviewed
• [Presentation] 長期実務実習のコミュニケーションスキル向上を目指したSimulated Patientを相手とした服薬指導シミュレーションの試み (2010)
  • Author(s): 岸本成史, 他
  • Organizer: 第20回日本医療薬学会年会
  • Place of Presentation: 幕張メッセ国際展示場
  • Year and Date: 2010-11-14
• [Presentation] 水晶体上皮細胞の上皮間葉系移行におけるロバスタチンの影響 (2010)
  • Author(s): 浦上千佳子, 岸本成史, 黒坂大二郎
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2010-04-17
• [Presentation] マクロファージ様に文化させたHL-60細胞の活性化を引き起こす牛乳カゼイン由来の生理活性ペプチド (2010)
  • Author(s): 岸本成史, 手塚優, 西郡秀夫
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山県桃太郎アリーナ
  • Year and Date: 2010-03-30
• [Presentation] マクロファージ様に分化させたHL-60細胞の活性化を引き起こす牛乳カゼイン由来の生理活性ペプチド (2010)
  • Author(s): 岸本成史, 他
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山県桃太郎アリーナ
  • Year and Date: 2010-03-30
• [Presentation] 血球系細胞の活性化を引き起こす牛乳カゼイン由来生理活性ペプチドの同定 (2009)
  • Author(s): 手塚優, 西郡秀夫, 岸本成史
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-24
• [Book] 食品安全ハンドブック (2010)
  • Author(s): 岸本成史
  • Publisher: 丸善