| Project/Area Number |
21590270
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Gunma University |
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Principal Investigator |
ISHIKAWA Ryoki 群馬大学, 大学院・医学系研究科, 講師 (20212863)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Akio 群馬大学, 大学院・医学系研究科, 講師 (30282388)
KOHAMA Kazuhiro 群馬大学, 大学院・医学系研究科, 教授 (30101116)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | アクチン / ミオシン / アクチン結合タンパク質 / アクチン結合蛋白質 |
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| Research Abstract |
To examine whether or not brain-specific actin binding protein drebrin affects the activity of myosin-V, we observed the sliding of myosin-V on F-actin in the presence or absence of drebrin. We found that myosin-V slides on drebrin-decorated F-actin as fast as "naked" F-actin, whereas sliding distance and attachment time were smaller in the presence of drebrin than that in the absence of drebrin. Drebrin inhibits the binding of leading head to F-actin, but does not inhibit the release of ADP from trailing head. These results suggest that drebrin modulates the affinity of myosin-V to F-actin, thus inhibits the activity of myosin-V, and affects the motility of the central zone of nerve cell growth cones.
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