Analysis of myosin sliding on wide variety of actin-rails.
Project/Area Number |
21590270
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General pharmacology
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Research Institution | Gunma University |
Principal Investigator |
ISHIKAWA Ryoki 群馬大学, 大学院・医学系研究科, 講師 (20212863)
|
Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Akio 群馬大学, 大学院・医学系研究科, 講師 (30282388)
KOHAMA Kazuhiro 群馬大学, 大学院・医学系研究科, 教授 (30101116)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | アクチン / ミオシン / アクチン結合タンパク質 / アクチン結合蛋白質 |
Research Abstract |
To examine whether or not brain-specific actin binding protein drebrin affects the activity of myosin-V, we observed the sliding of myosin-V on F-actin in the presence or absence of drebrin. We found that myosin-V slides on drebrin-decorated F-actin as fast as "naked" F-actin, whereas sliding distance and attachment time were smaller in the presence of drebrin than that in the absence of drebrin. Drebrin inhibits the binding of leading head to F-actin, but does not inhibit the release of ADP from trailing head. These results suggest that drebrin modulates the affinity of myosin-V to F-actin, thus inhibits the activity of myosin-V, and affects the motility of the central zone of nerve cell growth cones.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Utilization of myosin and actin bundles for the transport of molecular cargo2010
Author(s)
Takatsuki H, Rice KM, Asano S, Day BS, Hino M, Oiwa K, Ishikawa R, Hiratsuka Y, Uyeda TQ, Kohama K, Blough ER
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Journal Title
Small
Volume: 6
Pages: 452-457
Related Report
Peer Reviewed
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[Journal Article] Stimulatory effects of arachidonic acid on myosin ATPase activity and contraction of smooth muscle via myosin motor domain2010
Author(s)
Katayama K, Watanabe M, Tanaka H, Hino M, Miyakawa T, Ohki T, Ye LH, Xie C, Yoshiyama S, Nakamura A, Ishikawa R, Tanokura M, Oiwa K, Kohama K
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Journal Title
Am. J. Physiol. (Heart Cir. Physiol.)
Volume: 298
Issue: 2
Pages: H505-H514
DOI
Related Report
Peer Reviewed
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