The role of intracellular calcium and sodium-calcium exchanger in the automaticity of pulmonary vein myocardium.
| Project/Area Number |
21590293
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAMEKATA Eyuki 東邦大学, 薬学部, 講師 (30510309)
TAKAHARA Akira 東邦大学, 薬学部, 教授 (80377481)
KAWANISHI Toru 国立医薬品食品衛生研究所, 副所長 (40124383)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 肺静脈心筋 / ナトリウムカルシウム交換機構 / 自動能 / 細胞内カルシウム / 肺動脈心筋 / Na^+/Ca^<2+>交換機構 / Ca^<2+>チャネル / 筋小胞体 / イメージング / Na^+ / Ca^<2+>交換機構 / T型カルシウムチャネル |
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| Research Abstract |
We established glass microelectrode techniques to record the electrical activity of the pulmonary vein myocardium. We also established experimental protocols to constantly isolate pulmonary vein cardiomyocytes and load them with calcium sensitive fluorescent probes, and succeeded in high speed confocal imaging analysis of the intracellular calcium oscillation which precede their electrical activity. Pharmacological analyses led us to the understanding that increased intracellular calcium activates the sodium-calcium exchanger generates a depolarizing inward membrane current, which triggers electrical activity.
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Report
(4 results)
Research Products
(96 results)
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[Journal Article] Labdane-type diterpenoids from hairy root cultures of Coleus forskohlii, possible intermediates in the biosynthesis of forskolin2012
Author(s)
Yoshihisa Asada, Wei Li, Tomohiro Terada, Xinzhu Kuang, Qin Li, Takafumi Yoshikawa, Shogo Hamaguchi, Iyuki Namekata, Hikaru Tanaka, Kazuo Koike
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Journal Title
Phytochemistry
Volume: 79
Pages: 141-146
Related Report
Peer Reviewed
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[Journal Article] Autoimmunity against M2 muscarinic acetylcholine receptor induces myocarditis and leads to a dilated cardiomyopathy-like phenotype2012
Author(s)
Akihiro Yoshizawa, Shigenori Nagai, Yukiko Baba, Taketo Yamada, Minoru Matsui, Hikaru Tanaka, Shun-ichiro Miyoshi, Masayuki Amagai, Tsutomu Yoshikawa, Keniichi Fukuda, Satoshi Ogawa, Shigeo Koyasu
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Journal Title
European Journal of Immunology
Volume: 42
Pages: 1152-1163
Related Report
Peer Reviewed
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[Journal Article] Role of transient receptor potential vanilloid 2 in LPS-induced cytokine production in macrophages2010
Author(s)
Kenji Yamashiro, Tetsuo Sasano, Katsuyoshi Tojo, Iyuki Namekata, Junko Kurokawa, Naoki Sawada, Takayoshi Suganami, Yasutomi Kamei, Hikaru Tanaka, Naoko Tajima, Kazunori Utsunomiya, Yoshihiro Ogawa, Tetsushi Furukawa
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Journal Title
Biochem Biophys Res Commun
Volume: 398(2)
Pages: 284-289
Related Report
Peer Reviewed
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[Journal Article] Effects of S(+)-efonidipine on the rabbit sinus node action potential and calcium channel subunits CaV1.2, CaV1.3 and CaV 3.12010
Author(s)
Hikaru Tanaka, Iyuki Namekata, Toru Ogawa, Yayoi Tsuneoka, Chisa Komikado, Akira Takahara, Naoko Iida-Tanaka, Hiroko Nakaseko, Hiromichi Tsuru, Satomi Adachi-Akahane
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Journal Title
Eur J Pharmacol
Volume: 649(1-3)
Pages: 263-267
Related Report
Peer Reviewed
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